FastQCFastQC Report
Sat 23 Sept 2023
SRR20106161_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20106161_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163268802
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN102203726.259843812659323No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNN86431685.293827047251807No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN68016034.165892636365396No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN59930733.6706786150118256No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN55925873.4253861922745044No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN55715993.4125313175262963No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN55131013.376702059711322No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN28220531.7284704520585628No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN20773091.2723245191693144No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN19536641.1965935782391544No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN18336501.1230865771894376No Hit
CACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNN18163841.112511378628233No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN13516210.8278501363659176No Hit
CTCTAGCATCACCAGTATTAGAGGNNNNNNNNNNN13097140.8021826484645854No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN12579210.7704601152153979No Hit
GCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNN10766890.6594578920227515No Hit
CACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNN10336930.6331234059033519No Hit
CCTCTAGCATCACCAGTATTAGAGGNNNNNNNNNN9776230.5987812662458318No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN9695120.5938133851193445No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATNNNN8957630.548643089817No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN8884350.5441547859216852No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN7915390.48480725668581803No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN7899470.48383217756445596No Hit
TCTAGCATCACCAGTATTAGAGGNNNNNNNNNNNN7194130.4406310275982793No Hit
ATGTACACGGAGTCGACCCAACGCGACNNNNNNNN6083020.3725769972881898No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTNN5935840.36356241531067274No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN5901250.36144382317449725No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN5552070.34005700611437084No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN5488350.3361542396813814No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN5236080.32070303302648107No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACNNNNN5034430.3083522349848565No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGANNNNNN4690760.28730289819851806No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN4670040.28603382537222266No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNN4370960.2677155676073375No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNN4346470.2662155872252924No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN4345140.2661341264695505No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4339510.26578929635313914No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN3960140.24255338138635943No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN3752520.229836928674224No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN3661730.22427616024278782No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN3633440.22254343484433722No Hit
ACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNNN3451760.21141577311261217No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN3247380.19889776615130672No Hit
GAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNN3147790.19279800926082621No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN2749970.16843205599070912No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNNN2714150.16623812796764442No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNNN2658130.1628069764363188No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN2600170.1592570024492493No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG2593660.15885827348693354No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN2552820.15635687704745946No Hit
AACCCCATTCGTGATGGGGATCGGGGATTNNNNNN2484320.15216134188330727No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN2477870.15176628784230314No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN2442730.14961400892743734No Hit
ACCCCATTCGTGATGGGGATCGGGGATTNNNNNNN2407210.147438455510931No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN2377660.1456285567649354No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNN2301160.14094303209256107No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN2215030.13566768254966433No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN2112030.12935906763130411No Hit
GTTGAACCCCATTCGTGATGGGGACCGGGGATTNN2110500.1292653571378566No Hit
GCATGCTCATAAGGAAAGGTTAAAANNNNNNNNNN2084680.12768391600006962No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN2025930.1240855555490632No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN1962710.12021341346033763No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCNNNN1953600.11965543790784965No Hit
TGCTAAGACTTCACCAGTCAAAGCGNNNNNNNNNN1813960.11110267104183198No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNNN1795730.10998610744997075No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN1769350.10837036704660821No Hit
CCTCTAGCATCACCAGTATTAGAGGCNNNNNNNNN1698330.10402048518736605No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNN1686660.10330571299224699No Hit
CGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG1654820.10135555474952282No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN1652920.10123918224132006No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNN1636890.10025736576421992No Hit
AAAACCTGCATTAAAAATTTCGGTTGGGNNNNNNN1634330.10010056912158882No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNN1632740.10000318370682967No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)