FastQCFastQC Report
Sat 23 Sept 2023
SRR20106162_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20106162_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156585165
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNN129716098.284059987419626No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN73246004.677710049991006No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN56912803.6346227307037675No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN56464583.6059980522420503No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN47974013.063764693162344No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN43158732.7562464170855523No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN40672562.5974721168509163No Hit
CACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNN26189871.6725639366922147No Hit
CACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNN24161611.5430331474887804No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN22663521.4473606104384154No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN22432261.43259165068415No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN22385801.4296245752271615No Hit
GCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNN19563371.249375699160262No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN17505341.1179437081411896No Hit
CTCTAGCATCACCAGTATTAGAGGNNNNNNNNNNN17300851.1048843611717625No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN15174480.969087972031067No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14046490.897051135080389No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN12920670.8251528808619898No Hit
CCTCTAGCATCACCAGTATTAGAGGNNNNNNNNNN10794660.6893794824049903No Hit
TCTAGCATCACCAGTATTAGAGGNNNNNNNNNNNN10778070.6883199950646666No Hit
ACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNNN9442160.6030047610193469No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATNNNN9400030.6003142123968129No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN9226800.5892512231283213No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN8274260.528419151328927No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN6427580.4104846075297108No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN6218760.397148733725829No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNN5771660.36859558183561003No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN5574510.35600498936154007No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN5483630.35020111898850703No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN5449360.3480125336266689No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN4909950.3135641872587355No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN4599940.29376601544597153No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGANNNNNN4410840.2816895202045481No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN4363160.2786445318750343No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN3987550.254656946588778No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNN3958570.2528061965512506No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTNN3751400.2395756967143088No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN3566960.22779680310072797No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN3497330.22335002169586116No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN3295300.21044777773168996No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN3292810.21028875883612602No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN2803210.17902142900957443No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN2734150.17461104952055964No Hit
GAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNN2732230.17448843254084767No Hit
CCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNN2678480.17105579573901525No Hit
TGCTAAGACTTCACCAGTCAAAGCGNNNNNNNNNN2563060.16368472709403856No Hit
CGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNN2465610.1574612767435536No Hit
GGGAGAGCGACGCTCAGACAGGCNNNNNNNNNNNN2447500.15630471762762457No Hit
ACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNNN2443020.15601861134162998No Hit
GGGAGAGCGACGCTCAGACAGGNNNNNNNNNNNNN2366780.1511496954389006No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNNN2256710.1441202938988505No Hit
TTGATCATCGACACTTCGAACGNNNNNNNNNNNNN2213050.1413320348706086No Hit
ATTGATCATCGACACTTCGAACGNNNNNNNNNNNN2197850.14036131711455552No Hit
CCTCTAGCATCACCAGTATTAGAGGCNNNNNNNNN2110360.13477394234632636No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNNN2091980.13360014021762534No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN2070610.13223538768822704No Hit
CCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN2006260.12812580297756815No Hit
CTAGCATCACCAGTATTAGAGGNNNNNNNNNNNNN1976000.12619330828689934No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN1954740.12483558068863036No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN1933190.12345933281738407No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNN1916530.1223953750663417No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN1847700.11799968406968821No Hit
GCATGCTCATAAGGAAAGGTTAAAANNNNNNNNNN1836280.11727036849244307No Hit
ATGCTCATAAGGAAAGGTTAAAANNNNNNNNNNNN1817460.11606846663922474No Hit
AACGATGCCGACCGGCGATGCGGCGGCNNNNNNNN1757550.11224243369414977No Hit
ACCCCATTCGTGATGGGGATCGGGGATTNNNNNNN1754520.11204892877304182No Hit
CACTCGCCGAATCCCGGGGCCGAGGNNNNNNNNNN1746030.11150673181587797No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN1731910.11060498611091286No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN1717430.1096802497222518No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN1697520.10840873718784279No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN1680200.10730262985002442No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN1673220.10685686603836321No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNNN1646650.10516002585557833No Hit
AACCCCATTCGTGATGGGGATCGGGGATTNNNNNN1637050.10454694095701851No Hit
TTGATCATCGACACTTCGAACGCANNNNNNNNNNN1570870.10032048693757163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)