FastQCFastQC Report
Sat 23 Sept 2023
SRR20106163_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20106163_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167038519
Total Bases5.8 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN94239705.641794513276306No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN83616635.005829224335975No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN68298074.088761706513933No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN65103393.897507616192406No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43897042.6279591236078907No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNN33220541.9887951712502911No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN31964161.913580184460328No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN31253281.8710223358721232No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN29695001.7777336735127542No Hit
CACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNN25037851.4989267236019974No Hit
CTCTAGCATCACCAGTATTAGAGGNNNNNNNNNNN22493781.3466223320622233No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN21273131.2735463728578675No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN21106531.2635726254253967No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN18197721.089432551781664No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN16359150.979363927430415No Hit
CCTCTAGCATCACCAGTATTAGAGGNNNNNNNNNN15482480.9268808232189846No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATNNNN13296410.7960086140371012No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN11667960.6985191242027236No Hit
TCTAGCATCACCAGTATTAGAGGNNNNNNNNNNNN10867340.6505888620815657No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN8514280.5097195575590562No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN8383740.5019045936344778No Hit
CACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNN7803200.46714973568461776No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNN7376620.441611913477274No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN6746060.403862536640426No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN6746040.4038613393118027No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN5970150.357411574033412No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN5315550.3182230081913023No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN5290450.31672036076900323No Hit
GCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNN5240540.31373242718944366No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN5238070.3135845571044604No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGANNNNNN5150440.30833846174127055No Hit
GAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNN5119240.3064706290888511No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN4983660.2983539383511895No Hit
ATGTACACGGAGTCGACCCAACGCGACNNNNNNNN4027610.2411186368336994No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN3861620.23118140792424052No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN3716170.2224738355109578No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN3650850.21856336022711026No Hit
TTGATCATCGACACTTCGAACGCANNNNNNNNNNN3536750.21173260043092215No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNN3382960.20252574198170423No Hit
TGCTAAGACTTCACCAGTCAAAGCGNNNNNNNNNN3382310.20248682880144547No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNN3098860.1855176888870764No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN3061910.18330562425544494No Hit
TTCACTGACCCGGTGAGGCGGGGNNNNNNNNNNNN3027770.18126178429539358No Hit
ACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNNN2874040.17205851783204568No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN2872110.1719429756198928No Hit
GGGAGAGCGACGCTCAGACAGGCNNNNNNNNNNNN2846440.1704062043318284No Hit
GCATGCTCATAAGGAAAGGTTAAAANNNNNNNNNN2793980.16726561135279222No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN2760230.16524511930089608No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN2720780.1628833885913464No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNNN2693450.16124723902754431No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNNN2602440.155798795127009No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN2599230.155606623882962No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN2569980.15385553077131867No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN2493820.14929610337361768No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN2365820.14163320018420422No Hit
TAAACCATTCGTAGACGACCTGCTTNNNNNNNNNN2322160.13901943179944024No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN2220220.1329166478062464No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNNN2140290.1281315239630447No Hit
CCTCTAGCATCACCAGTATTAGAGGCNNNNNNNNN1983620.11875225019206499No Hit
GGGAGAGCGACGCTCAGACAGGNNNNNNNNNNNNN1954050.11698199982244815No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNNN1947520.11659107202692573No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNN1871400.11203403928647142No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN1869120.11189754382340998No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN1859310.11131025413365883No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTNN1838240.11004886842896398No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN1833490.10976450288091935No Hit
ACCCCATTCGTGATGGGGATCGGGGATTNNNNNNN1765130.10567203364632322No Hit
CACTCGCCGAATCCCGGGGCCGAGGNNNNNNNNNN1692020.10129519886368245No Hit
AACCTGCATTAAAAATTTCGGTTGGGNNNNNNNNN1676800.10038403178131627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)