FastQCFastQC Report
Sat 23 Sept 2023
SRR20106164_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20106164_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164056434
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNN1700677710.366418789768405No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN107842296.573487389101729No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN69391514.2297341413625995No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN52125173.177270694546488No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN47747692.910443000364131No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN28375161.7295975115489832No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN27514191.677117399735752No Hit
CACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNN26057251.5883101542972704No Hit
GCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNN25350191.5452115703063496No Hit
CACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNN22920541.3971131421764293No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN22513081.372276566733128No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21471241.3087715901468393No Hit
CTCTAGCATCACCAGTATTAGAGGNNNNNNNNNNN20495291.2492829144390643No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN19517491.1896814726571467No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN19134181.1663169516411651No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN17600341.0728222947964356No Hit
CCTCTAGCATCACCAGTATTAGAGGNNNNNNNNNN15337050.9348642796904875No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN12724080.7755916479325645No Hit
TCTAGCATCACCAGTATTAGAGGNNNNNNNNNNNN9565870.5830841111662831No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN8709450.5308813429408078No Hit
ACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNNN7610750.4639104858270905No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGANNNNNN7418630.4521998814139773No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN7012780.42746144293249727No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN6863280.4183487250490889No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN6658220.4058493676633249No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNN6022000.36706880999254193No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN5922950.36103125342831727No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN5532630.3372394404232875No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNNN5439120.3315395725351436No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN5301760.32316684391664885No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN5192740.316521569644748No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN5165140.3148392217278111No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN5157860.3143954719874016No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN4185800.2551439098084992No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNNN4025520.24537410096333068No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNN3862510.23543788596550866No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN3853620.23489599926327792No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN3801260.2317044145918715No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN3754610.22886088088444004No Hit
GGGAGAGCGACGCTCAGACAGGNNNNNNNNNNNNN3339240.20354215428088607No Hit
TGGTGACCTCCCGGGAGCGGGGGACCACCANNNNN3288610.20045602112746153No Hit
CCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN3155120.19231918694514596No Hit
CCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNN3096060.18871920622143964No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN3089790.18833702066204852No Hit
GAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNN3082690.18790424275587997No Hit
TCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNNN2977670.18150278702266562No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN2841230.17318613666806876No Hit
CCTCTAGCATCACCAGTATTAGAGGCNNNNNNNNN2738170.1669041520188108No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN2665320.1624636068829827No Hit
ATGCTCATAAGGAAAGGTTAAAANNNNNNNNNNNN2503720.1526133379200477No Hit
CGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNN2457440.14979235742744476No Hit
AACGATGCCGACCGGCGATGCGGCGGCNNNNNNNN2449750.14932361628681995No Hit
TGGTGACCTCCCGGGAGCGGGGGACCACCAGNNNN2444080.14897800350823182No Hit
ACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNNN2393280.1458815080669131No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN2271010.1384285848856132No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNNN2258540.13766848059125802No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN2250860.1372003489969799No Hit
ACGATGCCGACCGGCGATGCGGCGGCGNNNNNNNN2232200.13606293551400733No Hit
GCATGCTCATAAGGAAAGGTTAAAANNNNNNNNNN2225480.13565332036901398No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNN2222160.13545095098190418No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNN2217490.1351662928379877No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN2182410.13302800425370698No Hit
CACTCGCCGAATCCCGGGGCCGAGGNNNNNNNNNN2075470.12650951562192314No Hit
TGCTAAGACTTCACCAGTCAAAGCGNNNNNNNNNN2059490.1255354605598705No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN2025070.12343740203447308No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATNNNN2012990.12270107004763982No Hit
GGGAGAGCGACGCTCAGACAGGCNNNNNNNNNNNN1986870.12110893499001692No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN1857750.11323847256121634No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN1757110.10710399812786373No Hit
CTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNNN1734520.10572703293063167No Hit
CTAGCATCACCAGTATTAGAGGNNNNNNNNNNNNN1716420.1046237540430752No Hit
ATGCTCATAAGGAAAGGTTAAAAANNNNNNNNNNN1697440.10346683507700771No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)