FastQCFastQC Report
Sat 23 Sept 2023
SRR20106165_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20106165_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102449120
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNN77291847.544412289729769No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51137824.9915333582172305No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN46318774.521148644322177No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN42983754.195619249828598No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN25597652.498571974068689No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN23489002.2927478537638977No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN21116662.061185103395715No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN15493751.5123360747266545No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN14442811.4097544224879628No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN14411141.406663131903915No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN13429381.3108340998927077No Hit
CACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNN13415111.3094412133554687No Hit
GCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNN12953081.2643427293470164No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN10783241.0525458881442806No Hit
CACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNN10456351.0206383422327103No Hit
CTCTAGCATCACCAGTATTAGAGGNNNNNNNNNNN10243430.9998553428277374No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN8691950.8484162675091791No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN6936280.6770463230918918No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN5992230.584898142609717No Hit
GGGAGAGCGACGCTCAGACAGGNNNNNNNNNNNNN5978220.583530634523752No Hit
CCTCTAGCATCACCAGTATTAGAGGNNNNNNNNNN5763300.5625524162628239No Hit
TCTAGCATCACCAGTATTAGAGGNNNNNNNNNNNN5431010.5301177794401748No Hit
GAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNN5313510.5186486716528166No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN5166370.5042864204202047No Hit
ACGATGCCGACCGGCGATGCGGCGGCGNNNNNNNN5129060.5006446126623636No Hit
AACGATGCCGACCGGCGATGCGGCGGCNNNNNNNN5085370.4963800567540258No Hit
ACGATGCCGACCGGCGATGCGGCGGCNNNNNNNNN4760320.46465211218993385No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN4617760.4507369121374591No Hit
ACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNNN4317920.42146970125268035No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN4295290.41926079989754916No Hit
TCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNNN4182690.40826997830728073No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN4022530.3926368523224016No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN3851670.37595930545816303No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN3596440.3510464511554614No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN3379360.329857396530102No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGANNNNNN3367810.32873000763696164No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN3066390.2993085738559784No Hit
TTGATCATCGACACTTCGAACGNNNNNNNNNNNNN2979500.290827290659012No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN2931450.28613715764469233No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN2825840.27582862595598673No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN2754650.2688798107782673No Hit
CCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNN2587710.2525848928717006No Hit
AGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNNN2585920.2524101719956208No Hit
CGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNN2502550.24427247398513524No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN2402910.23454667058145545No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNN2283440.22288527222098148No Hit
ATTGATCATCGACACTTCGAACGNNNNNNNNNNNN2243090.2189467318020887No Hit
GGGAGAGCGACGCTCAGACAGGCNNNNNNNNNNNN2204970.21522586040758573No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN2162820.2111116230183334No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNNN2019610.1971329768376732No Hit
ACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNNN1926750.18806896535568093No Hit
CACTCGCCGAATCCCGGGGCCGAGGNNNNNNNNNN1849730.1805510872128526No Hit
CTCCATCTAAGGCTAAATACCGNNNNNNNNNNNNN1788330.17455786833503303No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNN1688820.16484475415699032No Hit
CCTCTAGCATCACCAGTATTAGAGGCNNNNNNNNN1660190.162050196233994No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN1646190.1606836642423088No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNNN1617580.157891058507872No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNNN1510200.14740975813164622No Hit
GACCGGCTCCGGGACGGCTGGGNNNNNNNNNNNNN1433280.1399016409316156No Hit
CTAGCATCACCAGTATTAGAGGNNNNNNNNNNNNN1352980.132063603865021No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN1295340.12643739643639693No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN1295300.1264334920592778No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN1271840.12414357487892527No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN1200170.11714790717577662No Hit
GCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNNN1166400.11385163679297587No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNN1102510.1076153704394923No Hit
CGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNNNN1099460.10731766168416088No Hit
TTGGTGACTCTAGATAACCTCGNNNNNNNNNNNNN1099130.10728545057292829No Hit
CCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN1069770.1044196377675084No Hit
ATGTACACGGAGTCGACCCAACGCGACNNNNNNNN1063430.10380079399413093No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN1043110.10181737041762778No Hit
TTGATCATCGACACTTCGAACGCANNNNNNNNNNN1039930.10150697243665929No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGNNNNN1038340.10135177344617503No Hit
ATGCTCATAAGGAAAGGTTAAAAANNNNNNNNNNN1031610.10069486199588634No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)