FastQCFastQC Report
Sat 23 Sept 2023
SRR20106166_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20106166_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175658401
Total Bases6.1 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNN160965169.1635332602168No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN99409385.6592442737765785No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN60545403.4467693919176687No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN49302832.806744779602087No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN48280262.7485312245327793No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN46306452.636164836773164No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42840412.438847772501356No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN36831922.09679239878769No Hit
CACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNN31162041.7740136436742358No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN29029141.6525904730283865No Hit
CACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNN28910411.6458313314602018No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN26122771.487134680225172No Hit
GCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNN25737691.465212586103411No Hit
CTCTAGCATCACCAGTATTAGAGGNNNNNNNNNNN22572531.2850242215286931No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN20411371.1619922465308108No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN19881801.131844528176025No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN19475931.1087388869035646No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN17096760.9732958914956763No Hit
TCTAGCATCACCAGTATTAGAGGNNNNNNNNNNNN15620250.8892401337525554No Hit
CCTCTAGCATCACCAGTATTAGAGGNNNNNNNNNN12811730.729354811786087No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN11489470.6540803021427937No Hit
ACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNNN10076680.5736520395628558No Hit
GAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNN8412910.47893581816220676No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN7978830.454224218971457No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN6733690.38334004873470295No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN6422470.3656227065393815No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN6389430.3637417831214346No Hit
TTGATCATCGACACTTCGAACGNNNNNNNNNNNNN6210450.3535526888918908No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN6023590.34291499670431363No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN5928180.33748343183426793No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNN5780070.32905172579818714No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGANNNNNN5757480.32776570703270835No Hit
GGGAGAGCGACGCTCAGACAGGNNNNNNNNNNNNN5593520.31843168149982193No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN5500420.3131316218687428No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN5207300.296444688688701No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATNNNN5028310.28625502517240836No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN4684610.26668863961707134No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN4493400.2558033076937778No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN4096040.23318212944452343No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNN4074440.23195247006717315No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN4069900.2316940138832301No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN4063140.23130917604105936No Hit
ATTGATCATCGACACTTCGAACGNNNNNNNNNNNN3779860.2151824210218104No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN3701150.21070156502221601No Hit
CCTCTAGCATCACCAGTATTAGAGGCNNNNNNNNN3623050.20625543551429687No Hit
ACGATGCCGACCGGCGATGCGGCGGCGNNNNNNNN3612300.20564345225936562No Hit
CTAGCATCACCAGTATTAGAGGNNNNNNNNNNNNN3553150.20227612114037175No Hit
CCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNN3501290.19932380006123362No Hit
CGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNN3305690.1881885512552286No Hit
ACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNNN3299110.1878139605745358No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNNN3268910.18609471459324053No Hit
GGGAGAGCGACGCTCAGACAGGCNNNNNNNNNNNN3238610.18436977574445756No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN2982110.16976757063842338No Hit
TTGATCATCGACACTTCGAACGCANNNNNNNNNNN2975790.16940778141319868No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN2763280.15730986871501806No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN2660330.15144906163639735No Hit
TTCACTGACCCGGTGAGGCGGGGNNNNNNNNNNNN2602410.1481517527875026No Hit
TGCTAAGACTTCACCAGTCAAAGCGNNNNNNNNNN2571830.14641087390975396No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN2532100.1441490976568778No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNNN2447030.13930617528506364No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN2438880.13884220658481344No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN2436630.13871411706633946No Hit
CACTCGCCGAATCCCGGGGCCGAGGNNNNNNNNNN2428530.1382529947998331No Hit
AGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNNN2346520.13358427417314359No Hit
AACGATGCCGACCGGCGATGCGGCGGCNNNNNNNN2271640.1293214549983294No Hit
CCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN2195170.12496811923046026No Hit
TCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNNN2153050.12257028344462728No Hit
ATGCTCATAAGGAAAGGTTAAAANNNNNNNNNNNN2059030.11721784943266107No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNN2030630.11560107506614499No Hit
ACCCCATTCGTGATGGGGATCGGGGATTNNNNNNN1939480.11041202635107672No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN1896620.10797206334583452No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN1889930.1075912105109052No Hit
AACCCCATTCGTGATGGGGATCGGGGATTNNNNNN1878110.1069183135738552No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNNN1867100.10629152886345583No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN1862490.10602908767227137No Hit
TGGTGACCTCCCGGGAGCGGGGGACCACCANNNNN1846240.10510399670551482No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN1839200.10470321883437844No Hit
ATGTACACGGAGTCGACCCAACGCGACNNNNNNNN1836330.1045398335374805No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN1783370.10152489091597731No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)