FastQCFastQC Report
Sat 23 Sept 2023
SRR20106167_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20106167_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165751250
Total Bases5.8 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNN1764214410.643747181393806No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN89575655.404221687619249No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN75841114.575598072412727No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN56341033.3991315299281304No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35951602.1690092834895665No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN35824682.1613520260028203No Hit
CACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNN35229402.125437968039457No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN35017102.112629618177842No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN33637932.029422402545984No Hit
GCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNN30058021.8134415275902895No Hit
CACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNN26104061.5748937036673931No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN24094171.4536342863175995No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN20144141.2153235646789995No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN19159811.1559375871976834No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN18524211.1175909683939036No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN16450870.9925035256144373No Hit
CTCTAGCATCACCAGTATTAGAGGNNNNNNNNNNN14129370.8524442500433632No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN12209120.7365929367048514No Hit
ACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNNN11958810.721491391467636No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN11549390.6967905219417652No Hit
TCTAGCATCACCAGTATTAGAGGNNNNNNNNNNNN10006660.6037155074245292No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN8105010.4889863575689475No Hit
CCTCTAGCATCACCAGTATTAGAGGNNNNNNNNNN7666160.4625099358225051No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN6937570.41855310291777587No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN6927270.41793168980626094No Hit
GGGAGAGCGACGCTCAGACAGGNNNNNNNNNNNNN6567310.396214809843063No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN6502610.392311370200828No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN6088330.3673172902165142No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN6054130.36525395736080424No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNN5864150.3537922036787053No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGANNNNNN5698580.34380313873952684No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN5597410.3376994140315684No Hit
CCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNN5455800.329155888718788No Hit
ACGATGCCGACCGGCGATGCGGCGGCGNNNNNNNN5322890.3211372463254425No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN5274930.31824375381784453No Hit
CGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNN4950700.2986825137065331No Hit
ACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNNN4694160.28320510403390625No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN4563020.2752932481655493No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN4552810.2746772648773388No Hit
TCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNNN4143280.24996975890076242No Hit
AGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNNN4115180.24827444740235746No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNNN3983640.2403384589859805No Hit
TTGATCATCGACACTTCGAACGNNNNNNNNNNNNN3960970.23897074682694702No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNN3957710.238774066560584No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNNN3953220.23850317870905952No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN3717010.2242523057895491No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN3596490.21698116907112314No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN3400170.2051369145029072No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN3351230.20218429725266024No Hit
GAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNN3346140.2018772105791057No Hit
GGGAGAGCGACGCTCAGACAGGCNNNNNNNNNNNN3318660.20021930453013176No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN3313860.19992971395389175No Hit
CACTCGCCGAATCCCGGGGCCGAGGNNNNNNNNNN3241450.19556111944857127No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATNNNN3234670.19515207275963228No Hit
ATTGATCATCGACACTTCGAACGNNNNNNNNNNNN2990570.18042518533042737No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN2981770.17989426927398738No Hit
CCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN2587570.15611164320027754No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN2531310.15271740032126455No Hit
CTAGCATCACCAGTATTAGAGGNNNNNNNNNNNNN2478630.14953914374703056No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN2227880.13441105270699316No Hit
AACGATGCCGACCGGCGATGCGGCGGCNNNNNNNN2219030.13387712008205066No Hit
CCTCTAGCATCACCAGTATTAGAGGCNNNNNNNNN2187420.13197004547477018No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN2158120.13020233633230519No Hit
AAACCTGCATTAAAAATTTCGGTTGGGNNNNNNNN2122440.12804971304892118No Hit
TGGTGACCTCCCGGGAGCGGGGGACCACCANNNNN2062320.12442259108151524No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNN2004180.12091492522680823No Hit
TTCACTGACCCGGTGAGGCGGGGNNNNNNNNNNNN1972610.11901026387432975No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN1896080.11439310412440329No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN1789930.10798892919359582No Hit
ATGCTCATAAGGAAAGGTTAAAANNNNNNNNNNNN1778360.10729089524211732No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN1757210.10601488676555983No Hit
GGGCGCACCACCGGCCCGTCTCGCNNNNNNNNNNN1671340.10083423201936637No Hit
ACGATGCCGACCGGCGATGCGGCGGCNNNNNNNNN1668570.10066711412432788No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN1660240.10016455381181137No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)