FastQCFastQC Report
Sat 23 Sept 2023
SRR20106168_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20106168_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167786892
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNN160361109.557427167790914No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN108590476.4719280931671355No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN65008813.874486810328425No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN58190523.4681207397297755No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN45926832.7372120344180404No Hit
CACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNN36002242.1457123122585764No Hit
CACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNN33747472.011329347467739No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN31837411.8974908957727163No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN27671861.6492265677106648No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25749371.5346472953322243No Hit
CTCTAGCATCACCAGTATTAGAGGNNNNNNNNNNN24708061.472585832271093No Hit
GCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNN24185091.4414171281031893No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN21470361.2796208180553221No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN21451471.278494985174408No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN19024111.1338257579739899No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN17901111.0668956189974603No Hit
TCTAGCATCACCAGTATTAGAGGNNNNNNNNNNNN16769890.9994755728594102No Hit
CCTCTAGCATCACCAGTATTAGAGGNNNNNNNNNN16532320.9853165406985429No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN15755300.9390066060702763No Hit
ACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNNN11428920.6811569046764392No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN10697890.6375879469774075No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN9259310.5518494257584794No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN8636780.5147470041938675No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN7445580.4437521853614167No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN7347120.4378840273172233No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN7101310.42323389600660816No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGANNNNNN6356110.3788204146483624No Hit
TTGATCATCGACACTTCGAACGNNNNNNNNNNNNN5978120.3562924331419167No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN5379360.3206066895857395No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNN5379140.32059357771523656No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN5109550.30452617240207297No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN4783480.2850925923343285No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN4559560.27174709213875897No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN4532440.2701307561022109No Hit
TTGATCATCGACACTTCGAACGCANNNNNNNNNNN4475850.2667580254123785No Hit
TTCACTGACCCGGTGAGGCGGGGNNNNNNNNNNNN4127240.24598107461219318No Hit
CACTCGCCGAATCCCGGGGCCGAGGGCGNNNNNNN4027480.24003543733320956No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNN3852880.22962938010676068No Hit
ATTGATCATCGACACTTCGAACGNNNNNNNNNNNN3826760.2280726434815897No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN3587460.2138105043390398No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNNN3550920.21163274184731903No Hit
AACGATGCCGACCGGCGATGCGGCGGCNNNNNNNN3547230.2114128200193374No Hit
CTAGCATCACCAGTATTAGAGGNNNNNNNNNNNNN3494390.20826358712216922No Hit
ACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNNN3386130.20181135484647988No Hit
GAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNN3334100.19871039747252722No Hit
CCTCTAGCATCACCAGTATTAGAGGCNNNNNNNNN3268550.19480365605675562No Hit
CGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNN3224340.19216876607977218No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN3222190.19204062734531133No Hit
CCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNN3197240.19055362203145165No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN3156290.18811302613555772No Hit
GGGAGAGCGACGCTCAGACAGGCNNNNNNNNNNNN3083140.18375332919331983No Hit
GGGAGAGCGACGCTCAGACAGGNNNNNNNNNNNNN3026020.18034901081545748No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN2934530.17489626066856284No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATNNNN2902750.17300219137499728No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN2751260.16397347654547412No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN2729100.16265275358935669No Hit
ATGCTCATAAGGAAAGGTTAAAANNNNNNNNNNNN2670880.1591828758589795No Hit
CACTCGCCGAATCCCGGGGCCGAGGNNNNNNNNNN2553250.15217219709868635No Hit
ACGATGCCGACCGGCGATGCGGCGGCGNNNNNNNN2492200.14853365303411187No Hit
TGCTAAGACTTCACCAGTCAAAGCGNNNNNNNNNN2366600.1410479669651429No Hit
CCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN2307650.13753458166446042No Hit
ACTCGCCGAATCCCGGGGCCGAGGGCGNNNNNNNN2280580.1359212255984812No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN2278300.13578533894054132No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN2266020.13505345816883002No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN2205510.13144709778639918No Hit
GCATGCTCATAAGGAAAGGTTAAAANNNNNNNNNN2182200.13005783550719802No Hit
CTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNNN2111470.12584236914049282No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN2034810.12127347826432115No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN2027860.12085926235525No Hit
ATGCTCATAAGGAAAGGTTAAAAANNNNNNNNNNN1898190.11313100668197608No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGNNNNNN1842490.1098113194682693No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN1841820.10977138786264662No Hit
CCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNN1800120.10728609240821983No Hit
TTTCACTGACCCGGTGAGGCGGGGNNNNNNNNNNN1734970.10340319075699907No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN1692340.10086246784999152No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)