FastQCFastQC Report
Sat 23 Sept 2023
SRR20138951.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20138951.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24541930
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGGGACGAAGGACCCGTCAACGGCGGGGGAGATCGGAAGAGCACACGT1541150.627966097205884No Hit
AAGGCGGTCGAGGAATAAGAGCTGGAGATCGGAAGAGCACACGTCTGAAC1275770.5198327922865073Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCTGGTAGTGTAGTGGTATCACGTGACCAGATCGGAAGAGCACACGTC1160580.47289679336547696No Hit
GGGCTCGTAGATCAGGGGTAGATCACTCCCTAGATCGGAAGAGCACACGT1025240.4177503562270775No Hit
AGGCGGTCGAGGAATAAGAGCTGGAGATCGGAAGAGCACACGTCTGAACT1024700.4175303246321703Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGCTGGTAGTGTAGTGGTATCACGTGACCTAGATCGGAAGAGCACACGT893430.3640422737739045No Hit
CAATGGGACGAAGGACCCGTCAACGGCGGGGGAGATCGGAAGAGCACACG640560.26100636746987704No Hit
TATGACCCTCTTAAGGTAGCGTAGAGATCGGAAGAGCACACGTCTGAACT580050.23635060486277976Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTAAGGCGGCCAGAGCGGTGAGGTTCCAGATCGGAAGAGCACACGTCTGA504210.2054483897558179Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAAGGCGGCCAGAGCGGTGAGGTAGATCGGAAGAGCACACGTCTGAACT492600.20071771046531384Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGCCGTAGCTCAGTTGGTAGAGCACCTCGAGATCGGAAGAGCACACGT474650.1934036972642331No Hit
ATGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAA466920.19025398572972868Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATGACCCTCTTAAGGTAGCGTAGTAGATCGGAAGAGCACACGTCTGAAC461360.18798847523401788Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGCGGAGGAAGGAACGGGCAACGGTAGGTCAAGATCGGAAGAGCACACG442110.18014475634149393No Hit
TTAAGGCGGCCAGAGCGGTGAGGAGATCGGAAGAGCACACGTCTGAACTC434000.1768402077587215Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTAAGGCGGCCAGAGCGGTGAGGTTAGATCGGAAGAGCACACGTCTGAAC425660.17344194201515528Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGCGGTCGAGGAATAAGAGCTGGGAGATCGGAAGAGCACACGTCTGAA383190.15613686454162323Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGGACGAAGGACCCGTCAACGGCGGGGGTAGATCGGAAGAGCACACG370970.15115763104205743No Hit
TTGAAGATTGTCTCGAGCCCTAAACAGCCGGGAGATCGGAAGAGCACACG359600.1465247435715121No Hit
TAACGGACCTGGGTCTTATGTGAAGTAGATCGGAAGAGCACACGTCTGAA356930.1454368095744711Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCGGTCGAGGAATAAGAGCTGGGAGATCGGAAGAGCACACGTCTGAAC323150.1316726109152785Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTAAGGCGGCCAGAGCGGTGAGGTTCAGATCGGAAGAGCACACGTCTGAA316640.12902000779889763Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCGGTCGAGGAATAAGAGCTGAGATCGGAAGAGCACACGTCTGAACTC290700.11845034192502382Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGCTCGTAGATCAGGGGTAGATCACTCCCTTAGATCGGAAGAGCACACG276050.11248096624837574No Hit
TTAAGGCGGCCAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC275760.11236280113259225Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATGGGACGAAGGACCCGTCAACGGCGGGGGAGATCGGAAGAGCACACGTC273370.1113889575921698No Hit
ATGCTCGCTAGGTGTGACACAGGCTAGATCGGAAGAGCACACGTCTGAAC261350.10649121727590292Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGATGCTCGCTAGGTGTGACACAGGCTAGATCGGAAGAGCACACGTCTGA253540.10330890846807891Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)