FastQCFastQC Report
Sat 23 Sept 2023
SRR20138952.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20138952.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19878881
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGAAACTCGGCGGTACCTAGAGCCCGTGAGATCGGAAGAGCACACGTC4064902.044833408882522No Hit
TTAAGGCGGCCAGAGCGGTGAGGTAGATCGGAAGAGCACACGTCTGAACT1201660.6044907658534703Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTCGGCGGTACCTAGAGCCCGTGAGATCGGAAGAGCACACGTCTGAACT1068560.5375352868202189Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTCGATCGTAGTTCGGATTGAGGGCTGAGATCGGAAGAGCACACGTCTG1017040.5116183350561835Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAAGGCGGCCAGAGCGGTGAGGTTAGATCGGAAGAGCACACGTCTGAAC1011470.5088163664745515Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACGGCACGAAGCGAACGAACGGTGAGAAGATCGGAAGAGCACACGTCTG979200.49258305837235Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGAACTTGACGTGAACGTTCGTATAGTACGAGAGATCGGAAGAGCACAC931580.46862798766188096No Hit
TTAAGGCGGCCAGAGCGGTGAGGAGATCGGAAGAGCACACGTCTGAACTC825500.4152648230048764Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA811550.40824732539019676TruSeq Adapter, Index 7 (97% over 36bp)
TTAAGGCGGCCAGAGCGGTGAGGTTCAGATCGGAAGAGCACACGTCTGAA690060.3471322153394852Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTCGATCGTAGTTCGGATTGAGGGCTGAGATCGGAAGAGCACACGTCT561520.2824706279996344Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTCGGCGGTACCTAGAGCCCGTGAGATCGGAAGAGCACACGTCTGAAC561280.2823498968578765Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAA553080.27822491618114725Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGGCACGAAGCGAACGAACGGTGAGAGATCGGAAGAGCACACGTCTGA527970.2655934204747239Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCTGGTAGTGTAGTGGTATCACGTGACCAGATCGGAAGAGCACACGTC509740.25642288416536124No Hit
AGGGAAACTCGGCGGTACCTAGAGCCCGAGATCGGAAGAGCACACGTCTG492570.2477855770654294Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACTCGGCGGTACCTAGAGCCCGTGAGATCGGAAGAGCACACGTCTGAA458200.23049587147284598Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCGATCGTAGTTCGGATTGAGGGCTAGATCGGAAGAGCACACGTCTGA457960.23037514033108805Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAAGGCGGCCAGAGCGGTGAGGTTCCAGATCGGAAGAGCACACGTCTGA451370.2270600643969849Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCT443810.22325703343161013TruSeq Adapter, Index 7 (97% over 36bp)
TTAAGGCGGCCAGAGCGGTGAGGTTCCACCCGAGATCGGAAGAGCACACG390780.19658048156734778No Hit
ATCCTGCCGGAGGTCATTGCTATTGGGGTCCGAAGATCGGAAGAGCACAC374520.18840094671324809No Hit
AAACTCGATCGTAGTTCGGATTGAGGGCTGAGATCGGAAGAGCACACGTC370150.1862026338404058No Hit
AGAAGCAGGCAAGGGTGAGGTTGTTCGCCTAGATCGGAAGAGCACACGTC356130.1791499229760468No Hit
CGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCT346840.17447662169716696TruSeq Adapter, Index 7 (97% over 36bp)
AGAAGCAGGCAAGGGTGAGGTTGTTCGCCAGATCGGAAGAGCACACGTCT345640.17387296598837731Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGACTAGGTCGGGCAGTTAGGCCCTGCTCTTAGATCGGAAGAGCACACGT344000.17304796985303145No Hit
TTAAGGCGGCCAGAGCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAG326760.16437544950342023Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGAAGCAGGCAAGGGTGAGGTTGTTCGCCTAAGATCGGAAGAGCACACGT314400.1581577957028869No Hit
TTAAGGCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCT298380.15009899199054513TruSeq Adapter, Index 7 (97% over 36bp)
TTAAGGCGGCCAGAGCGGTGAGGTTCCACCAGATCGGAAGAGCACACGTC295270.14853451761193198No Hit
AACGGCACGAAGCGAACGAACGGTGAGATCGGAAGAGCACACGTCTGAAC291560.146668215378924Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTAAGGCGGCCAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC286590.14416807465168688Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGAACTTGACGTGAACGTTCGTATAGATCGGAAGAGCACACGTCTGAAC286370.14405740443840878Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAATGGGACGAAGGACCCGTCAACGGCGGGGGAGATCGGAAGAGCACAC283980.1428551234850694No Hit
ATCCGAACGGATGTCACGCGAACTCGGACCAGATCGGAAGAGCACACGTC281510.14161259881781071No Hit
AGAGCTGGGCAAGACCGGTGCCAGCCGCCGCGGAGATCGGAAGAGCACAC273300.13748258767684157No Hit
TTAAGGCGGCCAGAGCGGTGAGGTTCCACCCGTACCCATCCCGAACACGG270020.13583259540614986No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACA257840.12970548996193498No Hit
TTAAGGCGGCCAGAGCGGTGAGGTTCCACCCAGATCGGAAGAGCACACGT250790.1261590126727958No Hit
TTAAGGCGGCCAGAGCGGTGAGGTTCCACCCGTAGATCGGAAGAGCACAC244390.12293951555925106No Hit
ATAGGGAAACTCGGCGGTACCTAGAGCCCGTGAGATCGGAAGAGCACACG236880.11916163691507585No Hit
GGACTAGGTCGGGCAGTTAGGCCCTGCTCTAGATCGGAAGAGCACACGTC228890.11514229598738479No Hit
TAGGGAAACTCGGCGGTACCTAGAGCCCGTGAGATCGGAAGAGCACACGT222680.11201837769439839No Hit
AACTCGATCGTAGTTCGGATTGAGGGCTAGATCGGAAGAGCACACGTCTG222430.11189261608840055Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAGGAGGTCGTGAGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCAG219300.11031808078130756Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAACTTGACGTGAACGTTCGTATAGTACGAGAGATCGGAAGAGCACACG210800.10604218617738093No Hit
GGGCTCGTAGATCAGGGGTAGATCACTCCCTAGATCGGAAGAGCACACGT209780.10552907882490971No Hit
GGCTACTGTGCCAGCTGGTGGATAGCTCGGCTCAGATCGGAAGAGCACAC203030.10213351546296796No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)