FastQCFastQC Report
Sat 23 Sept 2023
SRR20138953.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20138953.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20956688
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTA4933782.3542746830987795TruSeq Adapter, Index 6 (97% over 36bp)
CTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGA3098111.47833951624417TruSeq Adapter, Index 6 (97% over 36bp)
GGCTACTGTGCCAGCTGGTGGATAGATCGGAAGAGCACACGTCTGAACTC1383840.6603333503843737Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCG1191080.5683531672562TruSeq Adapter, Index 6 (97% over 36bp)
AGAGCTGGGCAAGACCGGTGCCAGCCGCCGCGGAGATCGGAAGAGCACAC987060.47099999770956175No Hit
ACTCGATCGTAGTTCGGATTGAGGGCTGAGATCGGAAGAGCACACGTCTG956370.45635550808410186Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAATGGGACGAAGGACCCGTCAACGGCGGGGGAGATCGGAAGAGCACAC919980.43899112302478327No Hit
ATCCTGCCGGAGGTCATTGCTATTGGGGTCCGAGATCGGAAGAGCACACG654170.3121533326258424No Hit
ATGGGTTGCTATCTCGAAAGAGAACGCAGATCGGAAGAGCACACGTCTGA590020.2815425796289948Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGACTAGGTCGGGCAGTTAGGCCCTGCTCTAGATCGGAAGAGCACACGTC479100.22861436883538083No Hit
TTAAGGCGGCCAGAGCGGTGAGGTTAGATCGGAAGAGCACACGTCTGAAC478260.22821354213986483Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCCTGCCGGAGGTCATTGCTATTGGGGTCCAGATCGGAAGAGCACACGT424790.2026990142717208No Hit
TTAAGGCGGCCAGAGCGGTGAGGTTCAGATCGGAAGAGCACACGTCTGAA421500.20112910971428308Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCCTGCCGGAGGTCATTGCTATTGAGATCGGAAGAGCACACGTCTGAAC418080.1994971724539679Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTCGGCTCGAGAGCCGATGACGGACGTGCCAGATCGGAAGAGCACACG387260.18479065012563053No Hit
AGGAACTTGACGTGAACGTTCGTATAGTACGAGAGATCGGAAGAGCACAC384830.1836311157564592No Hit
GTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCT363270.17334323057154832TruSeq Adapter, Index 6 (97% over 36bp)
ACTCGATCGTAGTTCGGATTGAGGGCTAGATCGGAAGAGCACACGTCTGA337870.16122299477856425Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCTGGTAGTGTAGTGGTATCACGTGACCAGATCGGAAGAGCACACGTC321730.15352139612900664No Hit
TTAAGGCGGCCAGAGCGGTGAGGTTCCAGATCGGAAGAGCACACGTCTGA311360.148573095137934Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGTA296690.14157294320552943TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATGGGGGT289100.13795118770675976TruSeq Adapter, Index 6 (97% over 36bp)
TGACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCG285720.13633833743194537TruSeq Adapter, Index 6 (97% over 36bp)
AGGGAAACTCGGCGGTACCTAGAGCCCGTGAGATCGGAAGAGCACACGTC278020.1326640927230486No Hit
AGAAGCAGGCAAGGGTGAGGTTGTTCGCCAGATCGGAAGAGCACACGTCT267690.12773487871747674Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCGAATTAGATGAAAGGGAAACTGGAGATCGGAAGAGCACACGTCTGA263610.125788006196399Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAAGGCGGCCAGAGCGGTGAGGTAGATCGGAAGAGCACACGTCTGAACT263420.12569734301527036Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAAT260690.12439465625484333TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGGGGT248390.1185254082133589TruSeq Adapter, Index 6 (97% over 36bp)
AACTCGATCGTAGTTCGGATTGAGGGCTGAGATCGGAAGAGCACACGTCT222750.10629065050737024Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)