FastQCFastQC Report
Wed 28 Jun 2023
SRR2014726.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2014726.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87431452
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTCGGGGAATAGTGGTACTCCTACAGAAAATCTGTAGGCACCATCAAT12180581.3931576934122059No Hit
ATAACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATATCT9829761.1242819117312612No Hit
TTAACAAAGATTCTTTTTAAAATCCCCACTCTGTAGGCACCATCAATATC8878191.01544579175009No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTTCTGTAGGCACCATCAATATCT8636550.9878081402559802No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTTTGCTGTAGGCACCATCAATAT7894880.9029793992212323No Hit
ATATTCTTATTTGAAATTGAACATAGGTAACCTGTAGGCACCATCAATAT7529780.8612209711443429No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTTTGCCTGTAGGCACCATCAATA7432670.8501139841529797No Hit
ATAACAAAGATTCTTTTTAAAATCCCCACTCTGTAGGCACCATCAATATC6470230.7400346044807765No Hit
TTAACAAAGATTCTTTTTAAAATCCCCACTTCTGTAGGCACCATCAATAT4677780.5350225683087134No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTTTCTGTAGGCACCATCAATATC4599890.5261138749016773No Hit
CCCTTCGGGGAATAGTGGTACTCCTACAGAAAATCTGTAGGCACCATCAA4423140.5058980376992939No Hit
TTTCTTTATTGTAGATTTGTGGTGTTTAATCTGTAGGCACCATCAATATC4358210.4984716483949049No Hit
TCTTTATTGTAGATTTGTGGTGTTTAATCTGTAGGCACCATCAATATCTC3805600.4352667047094219No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3372350.3857135988088131Illumina PCR Primer Index 6 (96% over 29bp)
TCTCCCCCTCTTTTCGCCTTCACTTTATTGTCTGTAGGCACCATCAATAT3322420.3800028392528583No Hit
TTAACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATATCT3210300.36717907875989525No Hit
ATTACCATTATTTGTATGATCAATTTTCACTGTAGGCACCATCAATATCT3077580.35199918674574915No Hit
ATATTCTTATTTGAAATTGAACATAGGTAACTGTAGGCACCATCAATATC3069890.35111964056138517No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATCAAT2785870.31863476315136574No Hit
CACAGGATTATACATGGGCACTACGCTTTGCCTGTAGGCACCATCAATAT2772660.3171238652195779No Hit
TTAACAAAGATTCTTTTTAAAATCCCCACTTTCTGTAGGCACCATCAATA2767190.3164982322379823No Hit
CTCCCCCTCTTTTCGCCTTCACTTTATTGTCTGTAGGCACCATCAATATC2751220.3146716584324826No Hit
TTTTCTTTATTGTAGATTTGTGGTGTTTAATCTGTAGGCACCATCAATAT2646670.302713719085896No Hit
CAAAGCGTAGTGCCCATGTATAATCCTGCTGTAGGCACCATCAATATCTC2568550.2937787193560505No Hit
TCTTTATTGTAGATTTGTGTGGTGTTTAACTGTAGGCACCATCAATATCT2497160.28561346550666916No Hit
TGTTATGTTGTTGGTTTAAGCCCAAAAAAACTGTAGGCACCATCAATATC2233510.2554584132950234No Hit
AAACAGTCGGTGCGAAGTCGTAACATGGTACTGTAGGCACCATCAATATC2176390.24892529521298584No Hit
CTCCCCCTCTTTTCGCCTTCACTTTATTGTACTGTAGGCACCATCAATAT2164750.24759396652820087No Hit
TCTATGGAGACATAGCCAGGTGGGGAGTTTGCTGTAGGCACCATCAATAT2160700.2471307464961236No Hit
TCTCCCCCTCTTTTCGCCTTCACTTTATTGCTGTAGGCACCATCAATATC2117700.24221260788394547No Hit
TCTCCCCCTCTTTTCGCCTTCACTTTATTGTACTGTAGGCACCATCAATA2054550.23498980664303734No Hit
ATAACAAAGATTCTTTTTAAAATCCCCACTTCTGTAGGCACCATCAATAT2036610.23293791346390996No Hit
TTTTTGGTACATAAATAATCCTATCACCTTTCTGTAGGCACCATCAATAT1973590.22572998101415498No Hit
AAAAGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATCAATA1912520.21874508043169638No Hit
AAAAATATCCTTTTCCCTGTTCTACTAATAACTGTAGGCACCATCAATAT1876810.21466073787725728No Hit
TTTCTTTATTGTAGATTTGTGTGGTGTTTAACTGTAGGCACCATCAATAT1744770.19955862107837347No Hit
TTTTCTTTATTGTAGATTTGTGGTGTTTAACTGTAGGCACCATCAATATC1741380.19917088875522734No Hit
TTTTTGGTACATAAATAATCCTATCACCTTCTGTAGGCACCATCAATATC1721460.19689253244930668No Hit
ATTAAAATATCCTTTTCCCTGTTCTACTAATACTGTAGGCACCATCAATA1668980.19089011583611812No Hit
TTCTCCCCCTCTTTTCGCCTTCACTTTATTGTCTGTAGGCACCATCAATA1597280.18268940563860248No Hit
CTCCCCCTCTTTTCGCCTTCACTTTATTGTAACTGTAGGCACCATCAATA1596400.18258875536002764No Hit
ATATTCTTATTTGAAATTGAACATAGGTCTGTAGGCACCATCAATATCTC1588820.18172179046048556No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTCTGTAGGCACCATCAATATCTC1561530.17860048807150086No Hit
ATATTCTTATTTGAAATTGAACATAGGTACTGTAGGCACCATCAATATCT1541050.1762580815883053No Hit
TTCTCCCCCTCTTTTCGCCTTCACTTTATTGCTGTAGGCACCATCAATAT1458980.1668712993580388No Hit
TTTTTTTGGTACATAAATAATCCTATCACCTTCTGTAGGCACCATCAATA1354220.1548893411949741No Hit
GCATCTGTGGTCTAGTGGTAGAATACTTCTGTAGGCACCATCAATATCTC1295390.14816064132161502No Hit
AAAACAGTCGGTGCGAAGTCGTAACATGGTACTGTAGGCACCATCAATAT1215440.13901633476246053No Hit
TTTCTTTATTGTAGATTTGTGGTGTTTAACTGTAGGCACCATCAATATCT1201480.13741965534325107No Hit
AAATAACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATAT1198800.13711312949486415No Hit
GCTTTTCGCTTTAATACGCTTCCTCTATTATCTGTAGGCACCATCAATAT1160840.13277144247815992No Hit
AACGATTTCTACTTCAAAAAAAAAAAAAACTGTAGGCACCATCAATATCT1155280.13213551571807364No Hit
TTTCTTTATTGTAGATTTGTGTGGTGTTTACTGTAGGCACCATCAATATC1139260.13030322314674586No Hit
AATAACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATATC1115610.1275982469100479No Hit
AACCTGGTTGATCTTGCCAGTAGTCATATGCCTGTAGGCACCATCAATAT1108470.1267816071497932No Hit
GTTTCTTTATTGTAGATTTGTGGTGTTTAATCTGTAGGCACCATCAATAT1106060.12650596263687808No Hit
AACAAAGATTCTTTTTAAAATCCCCACTTTCTGTAGGCACCATCAATATC1104610.1263401184278628No Hit
AAATATCCTTTTCCCTGTTCTACTAATAACTGTAGGCACCATCAATATCT1087890.12442776313494142No Hit
TTTTATCTTTGGATAAGTATTTGTTAGGCCTTCTGTAGGCACCATCAATA1067080.12204761279728032No Hit
GCATCTGTGGTCTAGTGGTAGAATACTTCCTGTAGGCACCATCAATATCT1062050.12147230495497205No Hit
TTTTTTGGTTTTGTAATTGGAATGGTGGGACTGTAGGCACCATCAATATC1042470.11923283625668255No Hit
TCCCTTCGGGGAATAGTGGTACTCCTACAGAAAATCTGTAGGCACCATCA998650.11422090988492334No Hit
TCTTTATTGTAGATTTGTGTGGTGTTTACTGTAGGCACCATCAATATCTC982100.11232799839581756No Hit
AACAAAGATTCTTTTTAAAATCCCCACTTCTGTAGGCACCATCAATATCT963310.11017888619761226No Hit
TTCTTTATTGTAGATTTGTGGTGTTTAATCTGTAGGCACCATCAATATCT962750.1101148360203374No Hit
TAGTGACAAGAAATAACAATGCAAGGCCAACTGTAGGCACCATCAATATC955230.10925473363978903No Hit
ACTCCCAATAGGTAAAAAGGGCGAAATAACTCCTGTAGGCACCATCAATA942610.10781131714477303No Hit
ATTTTTTGTTTTGTAGCAATAGTAATTCGTTCTGTAGGCACCATCAATAT930790.10645940090300685No Hit
AAAATATCCTTTTCCCTGTTCTACTAATAACTGTAGGCACCATCAATATC917740.10496680302186907No Hit
TTTTTGGTTTTGTAATTGGAATGGTGGGACTGTAGGCACCATCAATATCT917640.10495536549021284No Hit
TTTTTTTGGTACATAAATAATCCTATCACCTTTCTGTAGGCACCATCAAT916960.10487759027495047No Hit
CTCCCCCTCTTTTCGCCTTCACTTTATTGCTGTAGGCACCATCAATATCT913010.10442580777452948No Hit
AAGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATCAATATC909550.10403006917922397No Hit
ATTTTCTTATTTGAAATTGAACATAGGTAACCTGTAGGCACCATCAATAT888090.10157557488579738No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)