FastQCFastQC Report
Wed 28 Jun 2023
SRR2014727.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2014727.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91352543
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATTCGTATG33515703.66883054366642No Hit
TTAACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATTCGT14463231.5832323354151179No Hit
AACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATTCGTAT8406430.9202184990077398No Hit
ATATTCTTATTTGAAATTGAACATAGGCTGTAGGCACCATCAATTCGTAT6594030.721822270453927No Hit
ATATTCTTATTTGAAATTGAACATAGGTCTGTAGGCACCATCAATTCGTA5637040.6170643766315296No Hit
ACAAAGATTCTTTTTAAAATCCCCCTGTAGGCACCATCAATTCGTATGCC5493520.6013538123399587No Hit
TAACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATTCGTA5458760.5975487732180592No Hit
AAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATTCGTATGC5430630.594469493859629No Hit
ATAACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATTCGT5399400.5910508698154139No Hit
GCATCTGTGGTCTAGTGGTAGAATACTTCTGTAGGCACCATCAATTCGTA5119880.5604529257603699No Hit
TTTTTGGTACATAAATAATCCTATCACCTCTGTAGGCACCATCAATTCGT4087470.44743910413090526No Hit
TTTATCTTTGGATAAGTATTTGTTAGGCCTCTGTAGGCACCATCAATTCG4014390.43943932682859194No Hit
TTATCTTTGGATAAGTATTTGTTAGGCCTCTGTAGGCACCATCAATTCGT3473160.3801930286713529No Hit
TGGTCCAAATCGTCTAGTGGTTAGGACTCCCTGTAGGCACCATCAATTCG3470790.3799335941857689No Hit
ACAAAGATTCTTTTTAAAATCCCCACTCTGTAGGCACCATCAATTCGTAT3236470.3542835145815262No Hit
GGTCCAAATCGTCTAGTGGTTAGGACTCCCTGTAGGCACCATCAATTCGT3128940.342512632625892No Hit
GGTTTCGTAGTGTAGTGGTTAGCACTGAGGCTGTAGGCACCATCAATTCG2865740.3137011741424648No Hit
AGACAGGTTAGTTTTACCCTACTGATGCTGTAGGCACCATCAATTCGTAT2801940.3067172415769532No Hit
CAAAGCGTAGTGCCCATGTATAATCCTGCTGTAGGCACCATCAATTCGTA2763600.30252031407598584No Hit
TTTTTTTGGTACATAAATAATCCTATCACCTCTGTAGGCACCATCAATTC2629630.28785515034868814No Hit
TTTTATCTTTGGATAAGTATTTGTTAGGCCTCTGTAGGCACCATCAATTC2548120.2789325744330949No Hit
TTTTATCTTTGGATAAGTATTTGTTAGGCCCTGTAGGCACCATCAATTCG2349760.25721889318395874No Hit
ATTTTTTGTTTTGTAGCAATAGTAATTCGTCTGTAGGCACCATCAATTCG2347420.2569627426792049No Hit
TTAACAAAGATTCTTTTTAAAATCCCCCTGTAGGCACCATCAATTCGTAT2152140.23558621679530037No Hit
TTTATCTTTGGATAAGTATTTGTTAGGCCCTGTAGGCACCATCAATTCGT2127030.23283752484044148No Hit
ATCTTTGGATAAGTATTTGTTAGGCCTCTGTAGGCACCATCAATTCGTAT2040090.223320548394586No Hit
CTAACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATTCGT1979570.21669566439984053No Hit
AGACAGGTTAGTTTTACCCTACTGATGACTGTAGGCACCATCAATTCGTA1833090.20066108066635868No Hit
TTTTGGTACATAAATAATCCTATCACCTCTGTAGGCACCATCAATTCGTA1802750.19733988138677214No Hit
GCATCTGTGGTCTAGTGGTAGAATACTCTGTAGGCACCATCAATTCGTAT1780940.1949524273232328No Hit
TATATTCTTATTTGAAATTGAACATAGGCTGTAGGCACCATCAATTCGTA1774780.1942781165927696No Hit
TTTATCTTTGGATAAGTATTTGTTAGGCCTTCTGTAGGCACCATCAATTC1716480.1878962471794573No Hit
TTTTTGGTACATAAATAATCCTATCACCCTGTAGGCACCATCAATTCGTA1704160.1865476257185309No Hit
ACTATGCCGACTAGGTGTTGGATGAAAGTGCTGTAGGCACCATCAATTCG1679830.18388431726525664No Hit
TTATCTTTGGATAAGTATTTGTTAGGCCTTCTGTAGGCACCATCAATTCG1665600.1823266156914756No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTTTGCCTGTAGGCACCATCAATT1659670.1816774821473771No Hit
GGCTACTTAGTCTAGTGGTATGATTCTCTGTAGGCACCATCAATTCGTAT1619250.1772528653088508No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1592950.17437390878106152Illumina Single End Adapter 1 (95% over 22bp)
TTTTTTGGTACATAAATAATCCTATCACCTCTGTAGGCACCATCAATTCG1589440.1739896830239307No Hit
TTATCTTTGGATAAGTATTTGTTAGGCCCTGTAGGCACCATCAATTCGTA1536010.1681409131653839No Hit
TATATTCTTATTTGAAATTGAACATAGGTCTGTAGGCACCATCAATTCGT1499140.1641049007250953No Hit
AAACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATTCGTA1488180.16290515306180364No Hit
AACAAAGATTCTTTTTAAAATCCCCCTGTAGGCACCATCAATTCGTATGC1432030.15675863560798742No Hit
ATATTCTTATTTGAAATTGAACATAGGTACTGTAGGCACCATCAATTCGT1407700.15409532715471314No Hit
TTTTTGGTACATAAATAATCCTATCACCTTCTGTAGGCACCATCAATTCG1289290.14113345481800108No Hit
TTTTTTTGGTACATAAATAATCCTATCACCCTGTAGGCACCATCAATTCG1248510.1366694302095126No Hit
AAAAGATTCTTTTTAAAATCCCCCTGTAGGCACCATCAATTCGTATGCCG1210020.1324560828043944No Hit
GGCTACTTAGTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATTCG1194070.1307100996630165No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTTTGCTGTAGGCACCATCAATTC1179110.12907248788903447No Hit
ATATTCTTATTTGAAATTGAACATAGGTAACCTGTAGGCACCATCAATTC1154260.12635225710137046No Hit
TATAGTATTGTGACAGTAATCCAACTTGGCTGTAGGCACCATCAATTCGT1099750.12038526393293725No Hit
AAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATTCGTATGCC1095530.11992331729615892No Hit
ATCTTTGGATAAGTATTTGTTAGGCCTTCTGTAGGCACCATCAATTCGTA1053800.11535529996138148No Hit
TATTCTTATTTGAAATTGAACATAGGTCTGTAGGCACCATCAATTCGTAT1026890.11240956915671192No Hit
GGTTCTGTAGTGTAGTGGTTAGCACTGCAGACTGTAGGCACCATCAATTC1022010.11187537494166966No Hit
ATATTCTTATTTGAAATTGAACATAGGTAACTGTAGGCACCATCAATTCG1016050.111222957416741No Hit
AACTATGCCGACTAGGTGTTGGATGAAAGTGCTGTAGGCACCATCAATTC1010660.1106329355275857No Hit
GTATAGTGGCAAGTATTTCCGCCTGTAGGCACCATCAATTCGTATGCCGT998710.10932481649689818No Hit
TCTTTATTGTAGATTTGTGGTGTTTAATCTGTAGGCACCATCAATTCGTA973260.1065389060926306No Hit
ATAACAAAGATTCTTTTTAAAATCCCCCTGTAGGCACCATCAATTCGTAT965320.1056697458329102No Hit
GTAACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATTCGT958820.10495821665303832No Hit
CACAGGATTATACATGGGCACTACGCTTTGCCTGTAGGCACCATCAATTC937390.10261235968001461No Hit
GTATAGTGGCAAGTATTTCCCTGTAGGCACCATCAATTCGTATGCCGTCT933950.10223579654482087No Hit
ATATTCTTATTTGAAATTGAACCTGTAGGCACCATCAATTCGTATGCCGT933390.10217449556932422No Hit
GGTTTCGTAGTGTAGTGGTTAGCACTGACTGTAGGCACCATCAATTCGTA931770.10199716060449461No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)