FastQCFastQC Report
Wed 28 Jun 2023
SRR2014728.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2014728.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92086051
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAGATTCTTTTTAAAATCCCCACTTTTGCCTGTAGGCACCATCAATT14554851.5805705469984808No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATCAAT11277051.224620871189275No Hit
ATTTTTTGTTTTGTAGCAATAGTAATTCGTCTGTAGGCACCATCAATTCG11025541.1973083740989174No Hit
GGTTTCGTAGTGTAGTGGTTAGCACTGAGGCTGTAGGCACCATCAATTCG8805170.9561893364283804No Hit
ATAAGTATTTGTTAGGCCTTATAAGAAAAAAGCTGTAGGCACCATCAATT8234880.8942592184781603No Hit
AACTATGCCGACTAGGTGTTGGATGAAAGTGCTGTAGGCACCATCAATTC6265090.6803516854034711No Hit
GTATAGTGGCAAGTATTTCCGCCTGTAGGCACCATCAATTCGTATGCCGT6062680.6583711576468839No Hit
ACTATGCCGACTAGGTGTTGGATGAAAGTGCTGTAGGCACCATCAATTCG5949360.6460652764879666No Hit
TTTTATCTTTGGATAAGTATTTGTTAGGCCTCTGTAGGCACCATCAATTC5431360.5898135429870914No Hit
ATATTCTTATTTGAAATTGAACATAGGTAACCTGTAGGCACCATCAATTC5123330.5563633084884919No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTTTGCTGTAGGCACCATCAATTC4913210.5335455203741987No Hit
TTTTTTTGGTACATAAATAATCCTATCACCTCTGTAGGCACCATCAATTC4184830.4544477642981997No Hit
TTTTATCTTTGGATAAGTATTTGTTAGGCCCTGTAGGCACCATCAATTCG3586600.38948352774949596No Hit
ATATTCTTATTTGAAATTGAACATAGGTAACTGTAGGCACCATCAATTCG3399190.3691319111946716No Hit
TTTTATCTTTGGATAAGTATTTGTTAGGCCTTCTGTAGGCACCATCAATT3334920.3621525696655186No Hit
TTATTTTTTGTTTTGTAGCAATAGTAATTCGTCTGTAGGCACCATCAATT3195940.3470601644107857No Hit
TATTTTTTGTTTTGTAGCAATAGTAATTCGTCTGTAGGCACCATCAATTC3084280.33493454942486345No Hit
GTCTAGGCGGTTAGGATATTCGCTGTAGGCACCATCAATTCGTATGCCGT3021150.32807900514704447No Hit
GGCTACTTAGTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATTCG2939420.3192036109790396No Hit
TTTTTTTGGTACATAAATAATCCTATCACCTTCTGTAGGCACCATCAATT2916170.31667879861630727No Hit
GCATCTGTGGTCTAGTGGTAGAATACTTCTGTAGGCACCATCAATTCGTA2836580.3080357957797539No Hit
TTTATCTTTGGATAAGTATTTGTTAGGCCTTCTGTAGGCACCATCAATTC2725910.29601768893314795No Hit
ATTTTTTGTTTTGTAGCAATAGTAATTCGTTCTGTAGGCACCATCAATTC2658080.2886517524787766No Hit
ATATTCTTATTTGAAATTGAACATAGGTACTGTAGGCACCATCAATTCGT2605380.28292884445658334No Hit
AAGTATTTGTTAGGCCTTATAAGAAAAAAGCTGTAGGCACCATCAATTCG2591010.2813683475252946No Hit
TTTTTGGTACATAAATAATCCTATCACCTTTCTGTAGGCACCATCAATTC2470970.26833271414798754No Hit
ATATTCTTATTTGAAATTGAACATAGGTCTGTAGGCACCATCAATTCGTA2399780.2606019015844213No Hit
ACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATTCGTATG2136180.2319765020654431No Hit
AAAAGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATCAATT2067830.22455409668941062No Hit
GGTCCAAATCGTCTAGTGGTTAGGACTCCCTGTAGGCACCATCAATTCGT2000640.2172576604463145No Hit
ACAAAGATTCTTTTTAAAATCCCCCTGTAGGCACCATCAATTCGTATGCC1962270.21309090559220528No Hit
TTTTTGGTACATAAATAATCCTATCACCTTCTGTAGGCACCATCAATTCG1929620.20954530887636827No Hit
CAAAGCGTAGTGCCCATGTATAATCCTGCTGTAGGCACCATCAATTCGTA1900170.20634721321690733No Hit
TTTTTGGTACATAAATAATCCTATCACCTCTGTAGGCACCATCAATTCGT1864280.20244977168148953No Hit
TAAGTATTTGTTAGGCCTTATAAGAAAAAAGCTGTAGGCACCATCAATTC1794750.19489922529091838No Hit
ATTTTTTGTTTTGTAGCAATAGTAATTCGCTGTAGGCACCATCAATTCGT1789780.19435951271273433No Hit
TGGTCCAAATCGTCTAGTGGTTAGGACTCCCTGTAGGCACCATCAATTCG1788250.1941933637701545No Hit
AAAAGATTCTTTTTAAAATCCCCACTTTTGCCTGTAGGCACCATCAATTC1782070.19352225235502823No Hit
TTTTTATCTTTGGATAAGTATTTGTTAGGCCTCTGTAGGCACCATCAATT1777080.1929803678952418No Hit
TTTTTTTGGTACATAAATAATCCTATCACCTTTCTGTAGGCACCATCAAT1755370.19062279041589045No Hit
TTAAGTATTTGTTAGGCCTTATAAGAAAAAAGCTGTAGGCACCATCAATT1743330.18931531769127552No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1730410.18791228217615719Illumina Single End Adapter 1 (95% over 22bp)
TTTATCTTTGGATAAGTATTTGTTAGGCCTCTGTAGGCACCATCAATTCG1544760.16775179120233963No Hit
TTATTTTTTGTTTTGTAGCAATAGTAATTCGCTGTAGGCACCATCAATTC1534750.16666476446036327No Hit
GTATAGTGGCAAGTATTTCCGCCCTGTAGGCACCATCAATTCGTATGCCG1520080.16507168930503927No Hit
TTTTTATCTTTGGATAAGTATTTGTTAGGCCCTGTAGGCACCATCAATTC1491830.16200390654171934No Hit
TAAACAATACTATTTCGTTCTTTTTTGTTTCCTCTGTAGGCACCATCAAT1481640.1608973328653218No Hit
TTGTCTAAAATACTTCCATTAATCAAGAACGAACTGTAGGCACCATCAAT1417750.15395925708661348No Hit
AGTATTTGTTAGGCCTTATAAGAAAAAAGCTGTAGGCACCATCAATTCGT1382610.15014326111128384No Hit
TTTTTTGGTACATAAATAATCCTATCACCTCTGTAGGCACCATCAATTCG1374460.14925821935832603No Hit
TTTATTTTTTGTTTTGTAGCAATAGTAATTCGTCTGTAGGCACCATCAAT1329990.14442904061549997No Hit
TAGTATTATGAGTTTCTTAGATGGTAACATATCTGTAGGCACCATCAATT1262210.13706853386513448No Hit
TTTTTTGGTACATAAATAATCCTATCACCTTTCTGTAGGCACCATCAATT1259950.13682311124406887No Hit
TTTTTTGGTACATAAATAATCCTATCACCTTCTGTAGGCACCATCAATTC1243080.1349911291124863No Hit
TTTCTCTCTTCTTTTAAGAATGTACTTGCTTGCTGTAGGCACCATCAATT1232240.1338139692840124No Hit
TTCTCTCTTCTTTTAAGAATGTACTTGCTTGCTGTAGGCACCATCAATTC1231280.1337097189670996No Hit
AAAACTATGCCGACTAGGTGTTGGATGAAAGTGCTGTAGGCACCATCAAT1211990.13161493916163264No Hit
GTATAGTGGCAAGTATTTCCGCTGTAGGCACCATCAATTCGTATGCCGTC1198430.13014240343523908No Hit
GCGTAATTGGATAGCGCAATGCCCTGTAGGCACCATCAATTCGTATGCCG1194280.12969173800275136No Hit
ACATATAGTATTGTGACAGTAATCCAACTTGGCTGTAGGCACCATCAATT1191680.12940939339444582No Hit
TTTTTTTGTTTTGTAGCAATAGTAATTCGTCTGTAGGCACCATCAATTCG1174770.12757306749965858No Hit
TATAGTATTGTGACAGTAATCCAACTTGGCTGTAGGCACCATCAATTCGT1139480.12374078241231129No Hit
TCTTCTTTCTTTTTTTCTCCCCCTCTTTTCGCCCTGTAGGCACCATCAAT1138510.12363544615459729No Hit
TTATCTTTGGATAAGTATTTGTTAGGCCTTCTGTAGGCACCATCAATTCG1121060.12174047945654658No Hit
ACCCTTCTCGGCCTCTTGGCTAAGATCAATCTGTAGGCACCATCAATTCG1119690.12159170556678557No Hit
TATTTTTTGTTTTGTAGCAATAGTAATTCGCTGTAGGCACCATCAATTCG1099420.11939050356280345No Hit
GTATAGTGGCAAGTATTTCCCTGTAGGCACCATCAATTCGTATGCCGTCT1092910.11868355610123839No Hit
GCATCTGTGGTCTAGTGGTAGAATACTTCCTGTAGGCACCATCAATTCGT1079410.11721753601965187No Hit
TGGTCCAAATCGTCTAGTGGTTAGGACTCCACCTGTAGGCACCATCAATT1036890.11260011573305494No Hit
TGAAAGATATGATGAATTGTTTGGACAAGACTGTAGGCACCATCAATTCG983650.10681856690759821No Hit
CTCCCCCTCTTTTCGCCTTCACTTTATTGTACTGTAGGCACCATCAATTC975660.10595090020745922No Hit
TATATTCTTATTTGAAATTGAACATAGGTAACCTGTAGGCACCATCAATT959800.10422859809679536No Hit
ATATAGTATTGTGACAGTAATCCAACTTGGCTGTAGGCACCATCAATTCG942750.10237706903079165No Hit
AAGTGTATATGATTCTTTAAACATTTCTCTCTGTAGGCACCATCAATTCG932650.10128026882160471No Hit
TTTATCTTTGGATAAGTATTTGTTAGGCCTTACTGTAGGCACCATCAATT929680.10095774440365567No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)