FastQCFastQC Report
Wed 28 Jun 2023
SRR2014729.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2014729.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105623746
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAAGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATCAAT24024532.274538719730694No Hit
ATATTCTTATTTGAAATTGAACATAGGTAACCTGTAGGCACCATCAATAT13550661.2829179529383476No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTTTGCCTGTAGGCACCATCAATA10649511.008249603266296No Hit
TATACTAGCATTTATTTTGATTCTTTGGTCTGTAGGCACCATCAATATCT10384750.9831832701710844No Hit
ATAACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATATCT9985660.9453991529518372No Hit
ATTACCATTATTTGTATGATCAATTTTCACTGTAGGCACCATCAATATCT9785470.9264460285284712No Hit
AAATGTTATTATTAGATAGAAACTTCAATACTGTAGGCACCATCAATATC6695850.633934153405239No Hit
TAAACAATACTATTTCGTTCTTTTTTGTTTCCTTCTGTAGGCACCATCAA6551320.6202506773429528No Hit
ATTAAACAATAAAGTTAATAATAAATCAGCTGTAGGCACCATCAATATCT6397330.6056715693457795No Hit
TTAACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATATCT6322560.5985926687356837No Hit
TTAACAAAGATTCTTTTTAAAATCCCCACTCTGTAGGCACCATCAATATC6312350.5976260300406312No Hit
TTATTTTTTGTTTTGTAGCAATAGTAATTCGTCTGTAGGCACCATCAATA5293940.5012073705471495No Hit
ATAACAAAGATTCTTTTTAAAATCCCCACTCTGTAGGCACCATCAATATC4244790.401878380643686No Hit
TTTATTTTTTGTTTTGTAGCAATAGTAATTCGTCTGTAGGCACCATCAAT3985680.37734696514172106No Hit
ATTTTTTGTTTTGTAGCAATAGTAATTCGTCTGTAGGCACCATCAATATC3807250.36045398351995583No Hit
ATATTCTTATTTGAAATTGAACATAGGTAACTGTAGGCACCATCAATATC3679710.34837904726461794No Hit
ATTTTTTGTTTTGTAGCAATAGTAATTCGTTCTGTAGGCACCATCAATAT3461510.32772081384047863No Hit
TTTTATCTTTGGATAAGTATTTGTTAGGCCTCTGTAGGCACCATCAATAT3158270.2990113605703778No Hit
TTATTTTTTGTTTTGTAGCAATAGTAATTCGTTCTGTAGGCACCATCAAT3095420.2930609940685118No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTTTGCTTCTGTAGGCACCATCAA3042140.2880166738263572No Hit
TAGTATTATGAGTTTCTTAGATGGTAACATATACTGTAGGCACCATCAAT2786880.2638497596932417No Hit
TTTCTTTATTGTAGATTTGTGGTGTTTAATCTGTAGGCACCATCAATATC2675660.25331993053910434No Hit
TAAACAATACTATTTCGTTCTTTTTTGTTTCCTCTGTAGGCACCATCAAT2673440.2531097505290146No Hit
ATTAAAATATCCTTTTCCCTGTTCTACTAATAACTGTAGGCACCATCAAT2672470.2530179151192006No Hit
ATATTCTTATTTGAAATTGAACATAGGTACTGTAGGCACCATCAATATCT2582470.24449710389934476No Hit
ACATATAGTATTGTGACAGTAATCCAACTTGGCTGTAGGCACCATCAATA2510650.23769749654589983No Hit
TATTTTTTGTTTTGTAGCAATAGTAATTCGTCTGTAGGCACCATCAATAT2501480.23682932055827674No Hit
TTTTATCTTTGGATAAGTATTTGTTAGGCCTTCTGTAGGCACCATCAATA2495030.23621866242085374No Hit
TCTTTATTGTAGATTTGTGTGGTGTTTAACTGTAGGCACCATCAATATCT2482830.23506361912216217No Hit
ATTAAAATATTCCTTTCCCTGTTCTACTAATAACTGTAGGCACCATCAAT2425420.2296282883206964No Hit
TTTTTTTGGTACATAAATAATCCTATCACCTTCTGTAGGCACCATCAATA2355610.22301897908449492No Hit
TTTATTTTTTGTTTTGTAGCAATAGTAATTCGTTCTGTAGGCACCATCAA2220120.21019137117140305No Hit
TTTCTTTATTGTAGATTTGTGTGGTGTTTAACTGTAGGCACCATCAATAT2164040.2048819590246307No Hit
TTTTTTTGGTACATAAATAATCCTATCACCTTTCTGTAGGCACCATCAAT2128890.2015541088648759No Hit
TTAAACAATACTATTTCGTTCTTTTTTGTTTCCTTCTGTAGGCACCATCA2101180.19893064576596253No Hit
TTAACAAAGATTCTTTTTAAAATCCCCACTTCTGTAGGCACCATCAATAT2004690.1897953893814749No Hit
TTTTTTGGTACATAAATAATCCTATCACCTTTCTGTAGGCACCATCAATA1965560.186090730014442No Hit
TTTTTGGTACATAAATAATCCTATCACCTTTCTGTAGGCACCATCAATAT1937950.1834767344835507No Hit
TTATACTAGCATTTATTTTGATTCTTTGGTCTGTAGGCACCATCAATATC1898780.179768288089309No Hit
TTTTTTGGTACATAAATAATCCTATCACCTTCTGTAGGCACCATCAATAT1894120.1793270994194809No Hit
TATTTTTTGTTTTGTAGCAATAGTAATTCGTTCTGTAGGCACCATCAATA1886930.17864638127869464No Hit
TTTTTTTGGTACATAAATAATCCTATCACCTCTGTAGGCACCATCAATAT1726320.16344052027846087No Hit
ATATTCTTATTTGAAATTGAACATAGGTAACTCTGTAGGCACCATCAATA1680600.1591119481787741No Hit
TTTTATCTTTGGATAAGTATTTGTTAGGCCCTGTAGGCACCATCAATATC1605180.15197150837653495No Hit
AAATAACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATAT1595170.15102380481752656No Hit
TTTTTGGTACATAAATAATCCTATCACCTTCTGTAGGCACCATCAATATC1555010.14722162949986642No Hit
AGACAGGTTAGTTTTACCCTACTGATGAACTGTAGGCACCATCAATATCT1507000.14267625009247448No Hit
ATATTCTTATTTGAAATTGAACATAGGTCTGTAGGCACCATCAATATCTC1468000.13898389856387028No Hit
AAAAGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATCAATA1438710.13621084789020832No Hit
TTTTTATCTTTGGATAAGTATTTGTTAGGCCTCTGTAGGCACCATCAATA1436600.13601108220494282No Hit
ATTAAAATATTCCTTTCCCTGTTCTACTAATACTGTAGGCACCATCAATA1426860.13508894107959396No Hit
AAAACAATACTATTTCGTTCTTTTTTGTTTCCTTCTGTAGGCACCATCAA1380380.13068841546293955No Hit
ATTAAAATATCCTTTTCCCTGTTCTACTAATACTGTAGGCACCATCAATA1366730.1293960924279281No Hit
TTTTCTTTATTGTAGATTTGTGGTGTTTAATCTGTAGGCACCATCAATAT1354420.12823063480441227No Hit
TCTATGGAGACATAGCCAGGTGGGGAGTTTGCTGTAGGCACCATCAATAT1331320.12604362659131593No Hit
TATAGTATTGTGACAGTAATCCAACTTGGCTGTAGGCACCATCAATATCT1299260.12300832428344284No Hit
TTTTATTTTTTGTTTTGTAGCAATAGTAATTCGTCTGTAGGCACCATCAA1291290.12225375911208451No Hit
ATATAGTATTGTGACAGTAATCCAACTTGGCTGTAGGCACCATCAATATC1271580.1203877014549361No Hit
TGCACTTTACCTTTATAACAAACCTTGGAATCCTGTAGGCACCATCAATA1242610.11764494699894473No Hit
TATTCTTATTTGAAATTGAACATAGGTAACCTGTAGGCACCATCAATATC1224930.11597108097264416No Hit
TTTACTTACTACTGTGCATATAGAGGTGTCTGCTGTAGGCACCATCAATA1208100.11437768927453112No Hit
TTAACAAAGATTCTTTTTAAAATCCCCACTTTTGCCTGTAGGCACCATCA1200870.11369318410653605No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTTTGCTGTAGGCACCATCAATAT1196250.11325578246391677No Hit
TTTATCTTTGGATAAGTATTTGTTAGGCCTTCTGTAGGCACCATCAATAT1191650.11282027433490192No Hit
ATAAGTATTTGTTAGGCCTTATAAGAAAAAAGTCTGTAGGCACCATCAAT1181060.11181765888136556No Hit
AACCTGGTTGATCTTGCCAGTAGTCATATGCCTGTAGGCACCATCAATAT1180350.11175043914840892No Hit
TTTCTTTATTGTAGATTTGTGTGGTGTTTACTGTAGGCACCATCAATATC1175790.11131871804660289No Hit
TTTCTCTCTTCTTTTAAGAATGTACTTGCTTGACTGTAGGCACCATCAAT1130830.10706209946388381No Hit
AACTATGCCGACTAGGTGTTGGATGAAAGTGCTGTAGGCACCATCAATAT1117170.10576882967207014No Hit
TCTTTATTGTAGATTTGTGGTGTTTAATCTGTAGGCACCATCAATATCTC1103130.10443958312177264No Hit
TTCTCTCTTCTTTTAAGAATGTACTTGCTTGACTGTAGGCACCATCAATA1087030.10291530467022064No Hit
AACAAAGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATCAA1067470.10106344836510534No Hit
TATATTCTTATTTGAAATTGAACATAGGTAACCTGTAGGCACCATCAATA1064740.10080498375810304No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)