FastQCFastQC Report
Wed 28 Jun 2023
SRR2014730.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2014730.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56820161
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGAATAGTGGTACTCCTACAGAAAATCTGTAGGCACCATCAATATCT28044224.935610794907815No Hit
ATATTCTTATTTGAAATTGAACATAGGTCTGTAGGCACCATCAATATCTC14832412.6104132299097147No Hit
ATCTTTGGATAAGTATTTGTTAGGCCTTCTGTAGGCACCATCAATATCTC13187172.320861076053621No Hit
GGGGAATAGTGGTACTCCTACAGAAAATCTGTAGGCACCATCAATATCTC12450082.191137754783905No Hit
TATTCTTATTTGAAATTGAACATAGGTAACTCTGTAGGCACCATCAATAT9059911.5944886182212685No Hit
TCTTCTTTTAAGAATGTACTTGCTTGATTCTGTAGGCACCATCAATATCT7988391.4059076671746848No Hit
ATTTTTTGTTTTGTAGCAATAGTAATTCGTCTGTAGGCACCATCAATATC7193491.2660101403091766No Hit
TTTTATCTTTGGATAAGTATTTGTTAGGCCTTCTGTAGGCACCATCAATA7104781.2503977241458362No Hit
AACAAAGATTCTTTTTAAAATCCCCACTCTGTAGGCACCATCAATATCTC6007271.057242692430949No Hit
ATATTCTTATTTGAAATTGAACATAGGTAACTCTGTAGGCACCATCAATA5480320.9645027228979517No Hit
ATTTTTTGTTTTGTAGCAATAGTAATTCGTTCTGTAGGCACCATCAATAT5433430.9562503703570991No Hit
TCTTTATTGTAGATTTGTGGTGTTTAATCTGTAGGCACCATCAATATCTC5340630.939918139267504No Hit
TATTCTTATTTGAAATTGAACATAGGTAACCTGTAGGCACCATCAATATC5016010.8827870093504312No Hit
AAATGTTATTATTAGATAGAAACTTCAATACTGTAGGCACCATCAATATC4949670.8711115760478045No Hit
AAAAGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATCAATA4777320.8407790326394887No Hit
TTATCTTTGGATAAGTATTTGTTAGGCCTTCTGTAGGCACCATCAATATC4743550.8348357196664755No Hit
ATATTCTTATTTGAAATTGAACATAGGCTGTAGGCACCATCAATATCTCG4297110.7562650165669189No Hit
TTTATCTTTGGATAAGTATTTGTTAGGCCTTCTGTAGGCACCATCAATAT4151650.7306649483094565No Hit
AAAAGATTCTTTTTAAAATCCCCACTTTTGCCTGTAGGCACCATCAATAT4044300.7117720064186372No Hit
TTAATAATTAGACCAACAGATACAGCATTCTGTAGGCACCATCAATATCT3850910.6777365519960424No Hit
ATATTCTTATTTGAAATTGAACATAGGTAACCTGTAGGCACCATCAATAT3501600.6162601334410158No Hit
TTTCTTTATTGTAGATTTGTGGTGTTTAATCTGTAGGCACCATCAATATC3286670.5784337710693921No Hit
GGGGGAATAGTGGTACTCCTACAGAAAATCTGTAGGCACCATCAATATCT3259250.5736080191677035No Hit
TAACAAAGATTCTTTTTAAAATCCCCACTCTGTAGGCACCATCAATATCT3212780.5654295840520409No Hit
AAGTATTTGTTAGGCCTTATAAGAAAAAAGCTGTAGGCACCATCAATATC3029140.5331100698570707No Hit
ATTACCATTATTTGTATGATCAATTTTCACTGTAGGCACCATCAATATCT2848090.5012463797841051No Hit
TTTTTATCTTTGGATAAGTATTTGTTAGGCCTTCTGTAGGCACCATCAAT2697440.47473290334393803No Hit
TTAACAAAGATTCTTTTTAAAATCCCCACTCTGTAGGCACCATCAATATC2676150.4709859938622842No Hit
ATAAGTATTTGTTAGGCCTTATAAGAAAAAAGCTGTAGGCACCATCAATA2506480.4411251140242281No Hit
TCTTTATTGTAGATTTGTGTGGTGTTTACTGTAGGCACCATCAATATCTC2458010.4325946911695657No Hit
ATATTCTTATTTGAAATTGAACATAGGTACTGTAGGCACCATCAATATCT2223660.391350527852253No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2205900.38822487672993394Illumina PCR Primer Index 6 (96% over 29bp)
TTAACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATATCT2185730.38467508038212No Hit
AAACAAAGATTCTTTTTAAAATCCCCACTCTGTAGGCACCATCAATATCT2155700.37938998448103656No Hit
TTTCTTTATTGTAGATTTGTGGTGTTTAACTGTAGGCACCATCAATATCT2134240.3756131560415677No Hit
TATACTAGCATTTATTTTGATTCTTTGGTCTGTAGGCACCATCAATATCT2120290.3731580415620434No Hit
TCTTCTTTCTTTTTTTCTCCCCCTCTTTTCGCCTGTAGGCACCATCAATA2119250.3729750079377635No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTTTGCCTGTAGGCACCATCAATA2099740.3695413675438195No Hit
AAGTATTTGTTAGGCCTTATAAGAAAAAAGTTCTGTAGGCACCATCAATA2065510.3635170973908363No Hit
ACAAAGATTCTTTTTAAAATCCCCACTCTGTAGGCACCATCAATATCTCG1853560.32621519675032246No Hit
TCTCCCCCTCTTTTCGCCTTCACTTTATTGCTGTAGGCACCATCAATATC1766680.31092484936816706No Hit
TCTTTATTGTAGATTTGTGTGGTGTTTAACTGTAGGCACCATCAATATCT1741630.3065162029371934No Hit
AAGTATTTGTTAGGCCTTATAAGAAAAAAGTCTGTAGGCACCATCAATAT1694420.2982075323581009No Hit
AAAAATATCCTTTTCCCTGTTCTACTAATAACTGTAGGCACCATCAATAT1669020.2937372880728022No Hit
TTTAATAATTAGACCAACAGATACAGCATTCTGTAGGCACCATCAATATC1620280.28515934687337474No Hit
ATTTGTTAGGCCTTATAAGAAAAAAGTTCTGTAGGCACCATCAATATCTC1572940.2768277970912472No Hit
TGGGGAATAGTGGTACTCCTACAGAAAATACTGTAGGCACCATCAATATC1514880.2666095930280803No Hit
CTCCCCCTCTTTTCGCCTTCACTTTATTGCTGTAGGCACCATCAATATCT1513530.26637200130425537No Hit
ATTCTTATTTGAAATTGAACATAGGTAACTCTGTAGGCACCATCAATATC1475910.25975111193366734No Hit
CGGGGAATAGTGGTACTCCTACAGAAAATCTGTAGGCACCATCAATATCT1410990.24832558992573078No Hit
TTTCTTTATTGTAGATTTGTGTGGTGTTTACTGTAGGCACCATCAATATC1398500.24612742649567643No Hit
TTTCTTCTTTCTTTTTTTCTCCCCCTCTTTTCGCCTGTAGGCACCATCAA1340410.23590394261642447No Hit
TTAGATTTAGCAATTTTTGCATTACATTTACTGTAGGCACCATCAATATC1334160.2348039809320498No Hit
TATTCTTATTTGAAATTGAACATAGGTCTGTAGGCACCATCAATATCTCG1302260.2291897764950015No Hit
AACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATATCTCG1290060.2270426512871021No Hit
TTCTCCCCCTCTTTTCGCCTTCACTTTATTGCTGTAGGCACCATCAATAT1259040.22158332145521378No Hit
TTTTATCTTTGGATAAGTATTTGTTAGGCCTCTGTAGGCACCATCAATAT1246890.2194449959407894No Hit
TCTTTATTGTAGATTTGTGGTGTTTAACTGTAGGCACCATCAATATCTCG1239500.2181444012451848No Hit
AAAAATATTCCTTTCCCTGTTCTACTAATAACTGTAGGCACCATCAATAT1238960.21804936455565482No Hit
TATCTTTGGATAAGTATTTGTTAGGCCTTCTGTAGGCACCATCAATATCT1200300.21124544156078687No Hit
TATATTCTTATTTGAAATTGAACATAGGTCTGTAGGCACCATCAATATCT1176650.2070831865471131No Hit
AAAAATATCCTTTTCCCTGTTCTACTAATACTGTAGGCACCATCAATATC1156870.20360202780840414No Hit
TTTTTTGGTACATAAATAATCCTATCACCTTCTGTAGGCACCATCAATAT1131420.19912298382963045No Hit
TATTTTTTGTTTTGTAGCAATAGTAATTCGTCTGTAGGCACCATCAATAT1128280.19857036307940065No Hit
ATAAGTATTTGTTAGGCCTTATAAGAAAACTGTAGGCACCATCAATATCT1116410.19648131584843626No Hit
CACAGGATTATACATGGGCACTACGCTTTGCCTGTAGGCACCATCAATAT1073690.18896285774339852No Hit
TTTTTGGTACATAAATAATCCTATCACCTTCTGTAGGCACCATCAATATC1065850.1875830658065189No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTCTGTAGGCACCATCAATATCTC1040960.18320257839466522No Hit
ATAACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATATCT1033630.1819125433312306No Hit
TTTCTTTATTGTAGATTTGTGTGGTGTTTCTGTAGGCACCATCAATATCT994350.1749995041372727No Hit
ATCTTTGGATAAGTATTTGTTAGGCCTTACTGTAGGCACCATCAATATCT978640.1722346404474285No Hit
ATTTGAAATTGAACATAGGTAACTCTGTAGGCACCATCAATATCTCGTAT954610.16800550776334477No Hit
AACAAAGATTCTTTTTAAAATCCCCACTTCTGTAGGCACCATCAATATCT953960.1678911117481698No Hit
CTCCCCCTCTTTTCGCCTTCACTTTATTGTCTGTAGGCACCATCAATATC921900.16224874829200148No Hit
AAAAAGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATCAAT919880.1618932406756116No Hit
TTTTTTGGTACATAAATAATCCTATCACCTCTGTAGGCACCATCAATATC899890.15837512322430766No Hit
ATAGGTAACTATACATTTATTCAGTAATCCTGTAGGCACCATCAATATCT891580.1569126141687631No Hit
AAAAATATTCCTTTCCCTGTTCTACTAATACTGTAGGCACCATCAATATC866340.15247052890258442No Hit
AGGGGAATAGTGGTACTCCTACAGAAAATCTGTAGGCACCATCAATATCT857940.15099218039878484No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATCAAT856250.15069475075932995No Hit
AAAAACAGTTATAGTCTACTTGACATTTTTCTGTAGGCACCATCAATATC850680.14971446490621526No Hit
ATTCTTATTTGAAATTGAACATAGGTAACCTGTAGGCACCATCAATATCT825130.1452178215404916No Hit
AGATTCTTTTTAAAATCCCCACTTTTGCTCTGTAGGCACCATCAATATCT817080.143801070891017No Hit
ATATTCTTATTTGAAATTGAACATAGGTAACTGTAGGCACCATCAATATC807640.1421396887629375No Hit
TTTTTGTTTTGTAGCAATAGTAATTCGTTCTGTAGGCACCATCAATATCT793360.13962649630647825No Hit
TAATGTTATTATTAGATAGAAACTTCAATACTGTAGGCACCATCAATATC784320.13803551172619874No Hit
TAACAAAGATTCTTTTTAAAATCCCCACCTGTAGGCACCATCAATATCTC781620.1375603282785489No Hit
AAAAAGATTCTTTTTAAAATCCCCACTTTTGCCTGTAGGCACCATCAATA772610.13597462351435435No Hit
TCTCCCCCTCTTTTCGCCTTCACTTTATTGTCTGTAGGCACCATCAATAT772160.13589542627307938No Hit
ATTATAAGGATAACTACGGAAAAGCTGTCTGTAGGCACCATCAATATCTC769920.13550120000539947No Hit
TTTCTCCCCCTCTTTTCGCCTTCACTTTATTGCTGTAGGCACCATCAATA766400.13488170158475968No Hit
TTCACAAGGGAGAGAAAGAGAGGAAGGACTGTAGGCACCATCAATATCTC763580.13438539887276985No Hit
TAATATACGCATAATTAATGTAATATGTCTGTAGGCACCATCAATATCTC730950.12864271891098655No Hit
CTTCTTTTAAGAATGTACTTGCTTGATTCTGTAGGCACCATCAATATCTC728190.1281569758311667No Hit
TTGGGGAATAGTGGTACTCCTACAGAAAATCTGTAGGCACCATCAATATC722190.127101012614167No Hit
TTTCTTTATTGTAGATTTGTGTGGTGTTTAACTGTAGGCACCATCAATAT714310.1257141809225074No Hit
GGGGAATAGTGGTACTCCTACAGAAAATACTGTAGGCACCATCAATATCT685200.12059099938136394No Hit
TCTTTATTGTAGATTTGTGTGGTGTTTCTGTAGGCACCATCAATATCTCG674690.11874130381291952No Hit
AAAAGATTCTTTTTAAAATCCCCACTTTTGCTTCTGTAGGCACCATCAAT671260.11813764484053467No Hit
TATTCTTATTTGAAATTGAACATAGGTAACTGTAGGCACCATCAATATCT661590.11643578412247019No Hit
AATTCTTATTTGAAATTGAACATAGGTAACTCTGTAGGCACCATCAATAT651060.11458256867663574No Hit
TTTTTGGTACATAAATAATCCTATCACCTCTGTAGGCACCATCAATATCT650450.11447521241624077No Hit
GAAAAGCTTCTTAGAGGAACATTGTGTGTCTGTAGGCACCATCAATATCT647020.11387155344385597No Hit
TGTTATGTTGTTGGTTTAAGCCCAAAAAAACTGTAGGCACCATCAATATC636860.11208345572973649No Hit
TTTTTTTGTTTTGTAGCAATAGTAATTCGTCTGTAGGCACCATCAATATC628990.11069838397677191No Hit
TTTTTTTGGTACATAAATAATCCTATCACCCTGTAGGCACCATCAATATC620880.10927107369512734No Hit
TATTCTTATTTGAAATTGAACATAGGTACTGTAGGCACCATCAATATCTC619740.1090704406838974No Hit
TCTTTGGATAAGTATTTGTTAGGCCTTCTGTAGGCACCATCAATATCTCG616370.10847734134368256No Hit
GAAAAGCTTCTTAGAGGAACATTGTGTGTCTCTGTAGGCACCATCAATAT615150.10826262882289263No Hit
ACAAAGATTCTTTTTAAAATCCCCACTTTCTGTAGGCACCATCAATATCT614280.10810951415642768No Hit
TTTTTTTGGTACATAAATAATCCTATCACCTTCTGTAGGCACCATCAATA610440.10743369769754788No Hit
ATAAGTATTTGTTAGGCCTTATAAGAAACTGTAGGCACCATCAATATCTC603000.10612430330846827No Hit
TTTATCTTTGGATAAGTATTTGTTAGGCCTCTGTAGGCACCATCAATATC599640.10553296390694844No Hit
AAACAGTCGGTGCGAAGTCGTAACATGGTACTGTAGGCACCATCAATATC592670.10430628663653382No Hit
TTAATATACGCATAATTAATGTAATATGTCTGTAGGCACCATCAATATCT583990.10277865984927427No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)