FastQCFastQC Report
Mon 25 Sept 2023
SRR2015208.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2015208.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53675346
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTCGT774029614.420579608373647No Hit
GCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTCGTA26768574.9871257467068775No Hit
GAGGGTGCACCATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATG17276353.2186751064445867No Hit
TTTGCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTC13016862.425109658352272No Hit
TGAGGGTGCACCATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT11803792.199108320605889No Hit
TTGCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTCG11578212.157081577080099No Hit
TTTCCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATT9069801.6897515667621408No Hit
TCCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATTCG5393031.0047499274620417No Hit
TCGCCGGCGGATTGCAATGTCACCACGCGCGGCTGTAGGCACCATCAATT5231000.9745628840473614No Hit
TGAGGGTGCACCATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG4409710.8215522262306423No Hit
TTTACTTATTCAATGAAGCGGAACTGGGCCTGTAGGCACCATCAATTCGT4276530.7967400899474406No Hit
GAGGGTGCACCATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATGC3969180.739479164233054No Hit
TTGAGGGTGCACCATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA3746270.6979498557866771No Hit
TTTTACTTATTCAATGAAGCGGAACTGGGCCTGTAGGCACCATCAATTCG3195450.5953291852091648No Hit
TTCCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATTC2520320.4695489061216298No Hit
CGTTTTTTTACTTATTCAATGAAGCGGAACTGTAGGCACCATCAATTCGT2213550.41239603746569237No Hit
CCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATTCGT2165750.4034906454072974No Hit
TTTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGTAGGCACCATCAAT2140300.39874917620465833No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1858960.3463340506458962Illumina Single End Adapter 1 (95% over 22bp)
TTGAGGGTGCACCATCGACCGATCCTGCTGTAGGCACCATCAATTCGTAT1750930.32620749198337723No Hit
TACTTATTCAATGAAGCGGAACTGGGCCTGTAGGCACCATCAATTCGTAT1713460.3192266333970162No Hit
GATACGAATAGGCCTCCGGGTCCAGAACCTCTGTAGGCACCATCAATTCG1634110.3044433099695342No Hit
CCGCCGGCGGATTGCAATGTCACCACGCGCGGCTGTAGGCACCATCAATT1561190.29085792944865224No Hit
ACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTCGTATGC1512180.28172710800969963No Hit
ATTCCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATT1428900.26621160485858814No Hit
TCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGTAGGCACCATCAATTC1405290.26181293735861527No Hit
CGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCTGTAGGCACCA1400470.2609149459418482No Hit
GTTGCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTC1325640.24697372234917686No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATTCGT1317840.24552054121830905No Hit
CTTGCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTC1314060.24481630728565773No Hit
TCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCTGTAGGCACC1287250.23982146291148268No Hit
TGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCTGTAGGCACCA1282620.2389588694966214No Hit
TTCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTCGT1282380.23891415623105622No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTAGGCACCATCAATTC1242960.23157000236197828No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATTCGTA1226350.2284754717743226No Hit
TGCTACGAGTCGCTGAGCGTCAGCCCGACTGTAGGCACCATCAATTCGTA1169900.21795853910284996No Hit
TCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTCGTA1082270.20163260801336985No Hit
TTACTTATTCAATGAAGCGGAACTGGGCCTGTAGGCACCATCAATTCGTA1032890.19243285362333762No Hit
CGCCGGCGGATTGCAATGTCACCACGCGCGGCTGTAGGCACCATCAATTC1022460.1904896896239849No Hit
CTTGGTGATTCATAATAACTTGTCGAATCGCATCTGTAGGCACCATCAAT1017490.18956375241623966No Hit
GGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCTGTAGGCACCAT949100.17682233478290016No Hit
TGCTAGGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTCGT918300.17108413236870426No Hit
AGGGTGCACCATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATGC811180.15112711150478658No Hit
GGATACGAATAGGCCTCCGGGTCCAGAACCTCTGTAGGCACCATCAATTC803200.14964039542474492No Hit
CTTCCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATT795260.14816113155563077No Hit
TTTTACTTATTCAATGAAGCGGAACTGGGCTCTGTAGGCACCATCAATTC778460.1450312029660694No Hit
GGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCTGTAGGCACCA749510.13963766530727162No Hit
GCGCCGGCGGATTGCAATGTCACCACGCGCGGCTGTAGGCACCATCAATT742690.13836706334412824No Hit
TTTACTTATTCAATGAAGCGGAACTGGGCTCTGTAGGCACCATCAATTCG730730.13613885227679762No Hit
CGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATTCGTA722970.13469312335685735No Hit
ACTTATTCAATGAAGCGGAACTGGGCCTGTAGGCACCATCAATTCGTATG693360.12917662421775541No Hit
TTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGTAGGCACCATCAATT644160.12001040477689702No Hit
TACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTCGTATG625400.11651531785188678No Hit
TTTCCGGGGGGCATGCCTGTCCGAGCGTCCTGTAGGCACCATCAATTCGT598660.11153351484683491No Hit
CTTACTTATTCAATGAAGCGGAACTGGGCCTGTAGGCACCATCAATTCGT593440.1105610013207926No Hit
TTTTTACTTATTCAATGAAGCGGAACTGGGCCTGTAGGCACCATCAATTC548790.10224247087294044No Hit
CCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGTAGGCACCATCAATTCG548190.10213068770902753No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)