FastQCFastQC Report
Mon 25 Sept 2023
SRR2015210.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2015210.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37975769
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTCGT19900785.24038894380256No Hit
CTTGGTGATTCATAATAACTTGTCGAATCGCATCTGTAGGCACCATCAAT14004323.6876988587117223No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA12921073.402451178802989Illumina Single End Adapter 1 (95% over 22bp)
TTTCCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATT11482023.023512176935772No Hit
TCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCTGTAGGCACC6851341.8041346312170796No Hit
TTTGGTGATTCATAATAACTTGTCGAATCGCATCTGTAGGCACCATCAAT6462461.7017324915790382No Hit
TCCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATTCG4843141.2753237465711358No Hit
TTTGCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTC4595031.2099899807163879No Hit
CGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCTGTAGGCACCA4322191.1381441676664927No Hit
TCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCCTGTAGGCAC4051881.066964568907084No Hit
TGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCTGTAGGCACCA4036241.0628461532931697No Hit
TTGGTGATTCATAATAACTTGTCGAATCGCATCTGTAGGCACCATCAATT3980431.0481499400314973No Hit
TTTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGTAGGCACCATCAAT3552050.9353464310360641No Hit
TCTTGGTGATTCATAATAACTTGTCGAATCGCATCTGTAGGCACCATCAA3519130.926677745485549No Hit
CGTTTTTTTACTTATTCAATGAAGCGGAACTGTAGGCACCATCAATTCGT3229550.8504238584345718No Hit
TCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCTGTAGGCACCA3068570.808033670101585No Hit
TCGGCCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCTGTAGG3058030.8052582161009036No Hit
ATTCCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATT2890870.7612406742836465No Hit
TTGCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTCG2700660.7111534726261897No Hit
CGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCCTGTAGGCACC2576140.6783641431987855No Hit
GCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTCGTA2547430.6708040592937038No Hit
TGGCCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCTGTAGGC2220660.5847570854983872No Hit
TGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCCTGTAGGCACC2203840.5803279454327838No Hit
TTCCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATTC2049220.5396125092292403No Hit
GTGAATCATCGAATCTTTGAACGCATATTGCGCCCCCCTGTAGGCACCAT2047740.5392227870355963No Hit
CGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCTGTAGGCACCAT1932430.508858688286207No Hit
TCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGTAGGCACCATCAATTC1824440.4804221344405165No Hit
GAGGGTGCACCATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATG1665700.43862179591412614No Hit
GGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCTGTAGGCACCA1611480.42434427068481484No Hit
TCGCCGGCGGATTGCAATGTCACCACGCGCGGCTGTAGGCACCATCAATT1495070.39369051354825757No Hit
CGGCCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCTGTAGGC1472580.38776831615970697No Hit
TCGGCCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCTGTAGGC1451730.3822779730938431No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATTCGT1405110.37000172399405523No Hit
GATACGAATAGGCCTCCGGGTCCAGAACCTCTGTAGGCACCATCAATTCG1403470.3695698696713686No Hit
TTGAATCATCGAATCTTTGAACGCATATTGCGCCCCCCTGTAGGCACCAT1325250.3489725250856671No Hit
GGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCTGTAGGCACCAT1321100.3478797229886247No Hit
TCGGCCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCCTGTAG1314590.34616547198820385No Hit
GGATACGAATAGGCCTCCGGGTCCAGAACCTCTGTAGGCACCATCAATTC1297230.34159413598708166No Hit
TGAGGGTGCACCATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT1296860.34149670543867067No Hit
TGGCCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCTGTAGGCA1187390.3126704293993362No Hit
TGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCTGTAGGCACCAT1121870.29541732255639114No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATTCGTA1101070.28994014578085303No Hit
TGGCCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCCTGTAGG1092320.2876360449738358No Hit
GGATACGAATAGGCCTCCGGGTCCAGAACCTCCTGTAGGCACCATCAATT1041200.27417482974472485No Hit
CCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCTGTAGGCACC941420.2479001807705329No Hit
TTTGGTGATTCATAATAACTTGTCGAATCGCATGGCCCTGTAGGCACCAT928350.24445851247936545No Hit
CCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATTCGT904400.23815185941330114No Hit
GGCCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCTGTAGGCA895340.2357661276062639No Hit
GGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCCTGTAGGCACC894770.23561603189654964No Hit
TTTGGTGATTCATAATAACTTGTCGAATCGCATGGCCTGTAGGCACCATC837860.22063016024770954No Hit
CCTTGGTGATTCATAATAACTTGTCGAATCGCATCTGTAGGCACCATCAA826430.21762034627922874No Hit
CTTGGTGATTCATAATAACTTGTCGAATCGCATGGCCTGTAGGCACCATC826300.21758611392438165No Hit
GGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCCTGTAGGCACCA798740.21032885469679363No Hit
CCGGGGGTGCAATGCGGCCAGTCGGGACCGAGGACTGTAGGCACCATCAA791770.20849347382537534No Hit
CTTCCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATT785560.2068582205669094No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT751680.19793674224213867Illumina Single End Adapter 1 (95% over 22bp)
TTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGTAGGCACCATCAATT721380.18995797030469613No Hit
CGGCCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCTGTAGGCA683690.18003322065709848No Hit
AGTCGGATACGAATAGGCCTCCGGGTCCAGAACCTCCTGTAGGCACCATC664200.17490100068809666No Hit
CGGGGGTGCAATGCGGCCAGTCGGGACCGAGGACTGTAGGCACCATCAAT652670.17186485413896424No Hit
CTTGGTGATTCATAATAACTTGTCGAATCGCACTGTAGGCACCATCAATT649330.17098534594519996No Hit
GCGGCCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCTGTAGG648470.17075888575159595No Hit
TGAATCATCGAATCTTTGAACGCATATTGCGCCCCCCTGTAGGCACCATC644800.16979248004168132No Hit
ATTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGTAGGCACCATCAAT635610.16737251587979693No Hit
GGGGGGTGCAATGCGGCCAGTCGGGACCGAGGACTGTAGGCACCATCAAT627850.1653291076212308No Hit
TGGGGTGCAATGCGGCCAGTCGGGACCGAGGACTGTAGGCACCATCAATT626370.16493938542758674No Hit
TTGAGGGTGCACCATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA618230.16279591336254443No Hit
CGGCCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCCTGTAGG606280.15964916997467515No Hit
AGTCGGATACGAATAGGCCTCCGGGTCCAGAACCTCTGTAGGCACCATCA591080.15564661771562807No Hit
CTTGGTGATTCATAATAACTTGTCGAATCGCATGGCCCTGTAGGCACCAT584030.15379017077968848No Hit
GCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCTGTAGGCACC573310.15096731813383424No Hit
ATAATAACTTGTCGAATCGCATCTGTAGGCACCATCAATTCGTATGCCGT567580.1494584612624961No Hit
CGGATACGAATAGGCCTCCGGGTCCAGAACCTCCTGTAGGCACCATCAAT563060.1482682286170426No Hit
TTGGTGATTCATAATAACTTGTCGAATCGCATGGCCCTGTAGGCACCATC549080.14458693384194538No Hit
CGGATACGAATAGGCCTCCGGGTCCAGAACCTCTGTAGGCACCATCAATT544430.14336246884164477No Hit
TAGTCGGATACGAATAGGCCTCCGGGTCCAGAACCTCTGTAGGCACCATC534040.1406265137119409No Hit
CCGCCGGCGGATTGCAATGTCACCACGCGCGGCTGTAGGCACCATCAATT516010.1358787494204528No Hit
TTGGTGATTCATAATAACTTGTCGAATCGCATGGCCTGTAGGCACCATCA513780.13529153287192155No Hit
TGGTGATTCATAATAACTTGTCGAATCGCATCTGTAGGCACCATCAATTC512790.13503084032347046No Hit
CCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCCTGTAGGCAC499240.13146277564517522No Hit
GGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCTGTAGGCACCAT485660.12788681119268447No Hit
GATACGAATAGGCCTCCGGGTCCAGAACCTCCTGTAGGCACCATCAATTC476210.12539838232110584No Hit
CCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGTAGGCACCATCAATTCG463790.12212787580417397No Hit
TCGGATACGAATAGGCCTCCGGGTCCAGAACCTCCTGTAGGCACCATCAA448730.11816218915803917No Hit
GGCCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCTGTAGGCAC447180.11775403415793897No Hit
TCGACGGCCGGTCAAAGGCCCCCTGTAGGCACCATCAATTCGTATGCCGT443250.1167191637383301No Hit
TCGGATACGAATAGGCCTCCGGGTCCAGAACCTCTGTAGGCACCATCAAT439390.11570272612517735No Hit
CCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCTGTAGGCACCA436590.11496541386693183No Hit
GGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCTGTAGGCACCATC413120.10878515718799532No Hit
GTTCCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATT408700.10762125712319348No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC402970.10611240025185534Illumina Single End Adapter 1 (95% over 22bp)
CTCTAAGTCAGAATCCGTGCCGGAACGCGGCGATGTCTGTAGGCACCATC397490.10466937483214625No Hit
TGGATACGAATAGGCCTCCGGGTCCAGAACCTCTGTAGGCACCATCAATT392050.10323688244469782No Hit
GGCCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCCTGTAGGC389420.10254433557356007No Hit
TGTGAATCATCGAATCTTTGAACGCATATTGCGCCCCCCTGTAGGCACCA381590.10048249450853781No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)