FastQCFastQC Report
Mon 25 Sept 2023
SRR2015212.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2015212.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58084395
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTCGT28221414.858690531251983No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA21362493.6778363620728083Illumina Single End Adapter 1 (95% over 22bp)
TTTCCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATT19621313.378069101003807No Hit
ATTCCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATT13677572.3547753230450277No Hit
TTTGCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTC7379241.2704341673869548No Hit
TGAGGGTGCACCATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT5897011.0152485878522106No Hit
TTGAGGGTGCACCATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA5377840.925866577417222No Hit
TTTCCGGGGGGCATGCCTGTCCGAGCGTCCTGTAGGCACCATCAATTCGT5256900.9050451502507687No Hit
CGTTTTTTTACTTATTCAATGAAGCGGAACTGTAGGCACCATCAATTCGT4954860.8530449529516491No Hit
TTTACTTATTCAATGAAGCGGAACTGGGCTCTGTAGGCACCATCAATTCG4017050.6915885066892752No Hit
ATTCCGGGGGGCATGCCTGTCCGAGCGTCCTGTAGGCACCATCAATTCGT3626090.6242795504713443No Hit
TTTCCGGGGGGCATGCCTGTCCGAGCCTGTAGGCACCATCAATTCGTATG3572110.6149861765797854No Hit
ACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTCGTATGC3401150.5855531421132991No Hit
AATGAAGCGGAACTGGGCTCTGTAGGCACCATCAATTCGTATGCCGTCTT3326740.5727424723972764No Hit
TTGCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTCG3257760.5608666492953917No Hit
GCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTCGTA2663440.45854656831667095No Hit
TTTTACTTATTCAATGAAGCGGAACTGGGCTCTGTAGGCACCATCAATTC2652060.45658735018243707No Hit
ACTTATTCAATGAAGCGGAACTGGGCTCTGTAGGCACCATCAATTCGTAT2211980.3808217336170928No Hit
TCGCCGGCGGATTGCAATGTCACCACGCGCGGCTGTAGGCACCATCAATT2188390.37676040182565385No Hit
ATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATTCGTATGCCGTCT2180150.3753417763927816No Hit
GAGGGTGCACCATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATG2118970.3648088268802662No Hit
ATTCCGGGGGGCATGCCTGTCCGAGCCTGTAGGCACCATCAATTCGTATG2089530.35974033989680015No Hit
CTGGGGGCCTGAGTTGTAATTTGCAGAGGATCTGTAGGCACCATCAATTC2028780.3492814205949808No Hit
TTTTACTTATTCAATGAAGCGGAACTGGGCTTCTGTAGGCACCATCAATT1932040.33262634482118647No Hit
AATGAAGCGGAACTGGGCTTCTGTAGGCACCATCAATTCGTATGCCGTCT1923880.33122149245076926No Hit
CTTCCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATT1918370.3302728727741763No Hit
TCCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATTCG1818230.3130324418460414No Hit
TTCCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATTC1796710.3093274880456274No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1792650.30862850512603257Illumina Single End Adapter 1 (95% over 22bp)
CGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATTCGTA1786120.3075042789031374No Hit
TTTACTTATTCAATGAAGCGGAACTGGGCCTGTAGGCACCATCAATTCGT1783110.3069860674282654No Hit
TTTTACTTATTCAATGAAGCGGAACTGGGCCTGTAGGCACCATCAATTCG1739100.29940916144516955No Hit
AGTGAAAGCGAACGGAGGTGGGAACCCCCTGTAGGCACCATCAATTCGTA1733070.2983710168626186No Hit
CCGCCGGCGGATTGCAATGTCACCACGCGCGGCTGTAGGCACCATCAATT1667080.2870099619699921No Hit
TGCTACGAGTCGCTGAGCGTCAGCCCCTGTAGGCACCATCAATTCGTATG1590740.2738670171222409No Hit
CTTGGTGATTCATAATAACTTGTCGAATCGCATCTGTAGGCACCATCAAT1563260.269135970168924No Hit
CCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATTCGT1525110.26256794101066216No Hit
TTTTTACTTATTCAATGAAGCGGAACTGGGCTCTGTAGGCACCATCAATT1493880.2571912817547639No Hit
ATTCAATGAAGCGGAACTGGGCTCTGTAGGCACCATCAATTCGTATGCCG1409730.24270374168483635No Hit
ACTTATTCAATGAAGCGGAACTGGGCCTGTAGGCACCATCAATTCGTATG1376930.23705678607825734No Hit
CTTGGTGATTCATAATAACTTGTCGAATCGCCTGTAGGCACCATCAATTC1298230.22350753588808148No Hit
TATTCAATGAAGCGGAACTGGGCTCTGTAGGCACCATCAATTCGTATGCC1256590.21633865688021714No Hit
TGGCCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCTGTAGGC1217030.20952787749618462No Hit
GAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTCGTATGCCG1200980.20676465684113607No Hit
TTTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGTAGGCACCATCAAT1196580.20600713840610718No Hit
GTTGCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTC1187040.20436470070833998No Hit
CAATGAAGCGGAACTGGGCTCTGTAGGCACCATCAATTCGTATGCCGTCT1163770.20035846116672132No Hit
AGTTTTATGAGGTAAAGCGAATGATTAGAGGCCTGTAGGCACCATCAATT1158010.1993668006699562No Hit
TTTTTACTTATTCAATGAAGCGGAACTGGGCTTCTGTAGGCACCATCAAT1154540.1987693940859675No Hit
GGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATTCGTA1151560.19825634750951612No Hit
ACTTATTCAATGAAGCGGAACTGGGCTTCTGTAGGCACCATCAATTCGTA1129220.1944102198189376No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT1042310.1794475090943101Illumina Single End Adapter 1 (95% over 22bp)
TTTTTACTTATTCAATGAAGCGGAACTGGGCCTGTAGGCACCATCAATTC1013400.1744702686496089No Hit
TCGGCCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCTGTAGG1011090.17407257147121874No Hit
TTGGGGGCCTGAGTTGTAATTTGCAGAGGATCTGTAGGCACCATCAATTC1004760.17298277790446126No Hit
TGTTGCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATT974150.1677128598825898No Hit
AATGAAGCGGAACTGGGCCTGTAGGCACCATCAATTCGTATGCCGTCTTC969450.1669036924633544No Hit
GTTCCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATT933490.16071270088980008No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATTCGT923600.15901000604379198No Hit
TGCTACGAGTCGCTGAGCGTCAGCCCGACTGTAGGCACCATCAATTCGTA906500.1560660139440206No Hit
ATTCAATGAAGCGGAACTGGGCTTCTGTAGGCACCATCAATTCGTATGCC891770.15353004881948068No Hit
ACTTATTCAATGAAGCGGAACTGTAGGCACCATCAATTCGTATGCCGTCT872320.1501814730100916No Hit
ATTCAATGAAGCGGAACTGGGCCTGTAGGCACCATCAATTCGTATGCCGT870620.14988879543292136No Hit
TAGTGAAAGCGAACGGAGGTGGGAACCCCCTGTAGGCACCATCAATTCGT865480.14900387617018307No Hit
TATTCCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAAT825210.14207086085686182No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA821830.14148894896813508Illumina Single End Adapter 1 (95% over 22bp)
TTTCCGGGGGGCATGCCTGTCCGAGCGTCATCTGTAGGCACCATCAATTC815300.14036472274523992No Hit
TGACCCGCCGGCGGATTGCAATGTCACCACGCGCGGCTGTAGGCACCATC809370.1393437944907578No Hit
CTTGCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTC802700.13819546540856628No Hit
TTTACTTATTCAATGAAGCGGAACTGGGCTTCTGTAGGCACCATCAATTC790190.13604170276715458No Hit
TCGGCCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCCTGTAG766990.13204751465518408No Hit
TTTCCGGGGGGCATGCCTGTCCGAGCGTCTGTAGGCACCATCAATTCGTA756210.1301915944893633No Hit
TATTCAATGAAGCGGAACTGGGCCTGTAGGCACCATCAATTCGTATGCCG748780.12891242131384859No Hit
GGGGGGCCTGAGTTGTAATTTGCAGAGGATCTGTAGGCACCATCAATTCG747190.12863868169755405No Hit
ATTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGTAGGCACCATCAAT735010.12654173293876952No Hit
GTTTTTTTACTTATTCAATGAAGCGGAACTGTAGGCACCATCAATTCGTA732470.12610443820582104No Hit
TATTCAATGAAGCGGAACTGGGCTTCTGTAGGCACCATCAATTCGTATGC731530.12594260472197394No Hit
TGGCCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCCTGTAGG725810.12495783075643638No Hit
CTGGGGGCCTGAGTTGTAATTTGCCTGTAGGCACCATCAATTCGTATGCC723810.12461350419505962No Hit
AAATGAAGCGGAACTGGGCTCTGTAGGCACCATCAATTCGTATGCCGTCT722400.124370753969289No Hit
ACCCGCCGGCGGATTGCAATGTCACCACGCGCGGCTGTAGGCACCATCAA661480.11388256690975261No Hit
TGCTACGAGTCGCTGAGCGTCCTGTAGGCACCATCAATTCGTATGCCGTC656750.11306823459209656No Hit
AAATGAAGCGGAACTGGGCTTCTGTAGGCACCATCAATTCGTATGCCGTC646030.11122264422311708No Hit
TCTTGGTGATTCATAATAACTTGTCGAATCGCATCTGTAGGCACCATCAA645930.11120542789504824No Hit
TACTTATTCAATGAAGCGGAACTGGGCTCTGTAGGCACCATCAATTCGTA634510.10923932322958688No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC621360.10697537608853462Illumina Single End Adapter 1 (95% over 22bp)
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA619390.1066362144255785Illumina Single End Adapter 1 (95% over 22bp)
GTTTTCACGCCAGGTCGTACTCATAACCGCCTGTAGGCACCATCAATTCG616170.1060818486617619No Hit
CAATGAAGCGGAACTGGGCTTCTGTAGGCACCATCAATTCGTATGCCGTC613500.10562217270232391No Hit
CTTACTTATTCAATGAAGCGGAACTGGGCTCTGTAGGCACCATCAATTCG612650.1054758339137388No Hit
AGTTAAAAAGCTCGTAGTTGAACCTTGGGCTGTAGGCACCATCAATTCGT610110.1050385391807903No Hit
TATACGGTGAAACTGCGAATGGCTCATTAAATCCTGTAGGCACCATCAAT603100.10383167458316471No Hit
CAATGAAGCGGAACTGGGCCTGTAGGCACCATCAATTCGTATGCCGTCTT598760.10308448594497713No Hit
TCCGGGGGGCATGCCTGTCCGAGCGTCCTGTAGGCACCATCAATTCGTAT590230.10161593316070522No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)