FastQCFastQC Report
Mon 25 Sept 2023
SRR2015214.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2015214.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50708120
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTCGT28972885.713656905442363No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA21488354.237654639927491Illumina Single End Adapter 1 (95% over 22bp)
TGAGGGTGCACCATCGACCGATCCTGACTGTAGGCACCATCAATTCGTAT19006163.7481492116055573No Hit
GAGGGTGCACCATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATG18042273.558063284539044No Hit
TTTCCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATT15172252.9920750365030293No Hit
TTGAGGGTGCACCATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA11283992.2252826568999207No Hit
ATTCCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATT9924481.957177667008755No Hit
TTTGCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTC6232701.2291325334088505No Hit
GCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTCGTA3777970.745042411353448No Hit
CGTTTTTTTACTTATTCAATGAAGCGGAACTGTAGGCACCATCAATTCGT3395900.6696955043886462No Hit
TTGCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTCG3052750.602023896764463No Hit
TGAGGGTGCACCATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATG2727080.5377994688030241No Hit
TCCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATTCG2347400.4629238867463436No Hit
CTTGGTGATTCATAATAACTTGTCGAATCGCATCTGTAGGCACCATCAAT2204960.43483371105061674No Hit
TTGAGGGTGCACCATCGACCGATCCTGCTGTAGGCACCATCAATTCGTAT2038730.40205197905187573No Hit
AATGAAGCGGAACTGGGCTCTGTAGGCACCATCAATTCGTATGCCGTCTT1964530.3874192141219197No Hit
TTCCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATTC1796660.3543140625209532No Hit
ACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTCGTATGC1792960.3535843963452007No Hit
TTTACTTATTCAATGAAGCGGAACTGGGCTCTGTAGGCACCATCAATTCG1791560.3533083064408619No Hit
GAGGGTGCACCATCGACCGATCCTGCTGTAGGCACCATCAATTCGTATGC1673630.33005167614180925No Hit
TTTTACTTATTCAATGAAGCGGAACTGGGCTCTGTAGGCACCATCAATTC1612340.31796485454400597No Hit
AGTGAAAGCGAACGGAGGTGGGAACCCCCTGTAGGCACCATCAATTCGTA1592510.3140542382561215No Hit
CTTCCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATT1531470.30201671842695016No Hit
CCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATTCGT1525220.30078417421115194No Hit
ATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATTCGTATGCCGTCT1495320.2948876826827735No Hit
GATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA1494010.2946293414151422Illumina Single End Adapter 1 (95% over 22bp)
CTGGGGGCCTGAGTTGTAATTTGCCTGTAGGCACCATCAATTCGTATGCC1477770.2914266985248122No Hit
CGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATTCGTA1476990.2912728770066806No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1236510.2438485197242572Illumina Single End Adapter 1 (95% over 22bp)
TCGCCGGCGGATTGCAATGTCACCACGCGCGGCTGTAGGCACCATCAATT1182690.23323483497317588No Hit
CAATGAAGCGGAACTGGGCTCTGTAGGCACCATCAATTCGTATGCCGTCT1176760.23206539702122658No Hit
TGCTACGAGTCGCTGAGCGTCAGCCCGACTGTAGGCACCATCAATTCGTA1162980.22934788353423477No Hit
AATGAAGCGGAACTGGGCCTGTAGGCACCATCAATTCGTATGCCGTCTTC1146740.22614524064390476No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATTCGT1071330.21127385515377026No Hit
TTTACTTATTCAATGAAGCGGAACTGGGCCTGTAGGCACCATCAATTCGT1047300.2065349691528694No Hit
TTTTACTTATTCAATGAAGCGGAACTGGGCCTGTAGGCACCATCAATTCG1039790.20505394402316632No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT1033270.20376815389724565Illumina Single End Adapter 1 (95% over 22bp)
ACTTATTCAATGAAGCGGAACTGTAGGCACCATCAATTCGTATGCCGTCT1011710.19951636937042821No Hit
TTTTACTTATTCAATGAAGCGGAACTGGGCTTCTGTAGGCACCATCAATT950890.18752223509765298No Hit
GTTGCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTC950530.18745124055082305No Hit
ACTTATTCAATGAAGCGGAACTGGGCTCTGTAGGCACCATCAATTCGTAT902460.17797149647827606No Hit
AATGAAGCGGAACTGGGCTTCTGTAGGCACCATCAATTCGTATGCCGTCT900170.1775198922776076No Hit
GTGTAGTGGCTAACATCAGCCTGTAGGCACCATCAATTCGTATGCCGTCT890800.17567206198928298No Hit
GGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATTCGTA864090.17040466102864787No Hit
ACATACGACCATAGGGAGTGGAAAACCTGTAGGCACCATCAATTCGTATG855530.1687165684706907No Hit
GGCGCCGGTGAAATACCACTACCTTTATCTGTAGGCACCATCAATTCGTA841680.16598525048848192No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA816720.16106296190827032Illumina Single End Adapter 1 (95% over 22bp)
CAATGAAGCGGAACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTT815760.1608736431167237Illumina Single End Adapter 1 (95% over 21bp)
CCGCCGGCGGATTGCAATGTCACCACGCGCGGCTGTAGGCACCATCAATT792520.15629055070469974No Hit
TCGACGAGTCGAGTTGTTTGGGAATGCCTGTAGGCACCATCAATTCGTAT792270.15624124893606783No Hit
GTTCCGGGGGGCATGCCTGTCCGAGCGTCATTCTGTAGGCACCATCAATT782470.15430861960569628No Hit
TTTCCGGGGGGCATGCCTGTCCGAGCGTCATCTGTAGGCACCATCAATTC769340.1517192907171475No Hit
ATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGC743770.14667670582147396No Hit
ATTCAATGAAGCGGAACTGTAGGCACCATCAATTCGTATGCCGTCTTCTG742640.14645386182725764No Hit
CAATGAAGCGGAACTGGGCCTGTAGGCACCATCAATTCGTATGCCGTCTT741030.14613635843726802No Hit
CTGGGGGCCTGAGTTGTAATTTGCTGTAGGCACCATCAATTCGTATGCCG732860.14452517663837666No Hit
GAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTCGTATGCCG720020.14199303780144087No Hit
TAGTGAAAGCGAACGGAGGTGGGAACCCCCTGTAGGCACCATCAATTCGT700090.13806270080610364No Hit
CTGAGGGTGCACCATCGACCGATCCTGACTGTAGGCACCATCAATTCGTA691220.13631347405504288No Hit
GTGTAGTGGCTAACATCGCTCTGTAGGCACCATCAATTCGTATGCCGTCT676980.13350524531376828No Hit
TTTCCGGGGGGCATGCCTGTCCGAGCGTCCTGTAGGCACCATCAATTCGT657370.12963801458227991No Hit
ACTTATTCAATGAAGCGGAACTGGGCCTGTAGGCACCATCAATTCGTATG641790.1265655283611382No Hit
ATTCAATGAAGCGGAACTGGGCTCTGTAGGCACCATCAATTCGTATGCCG635550.1253349562160853No Hit
GGCGCCGGTGAAATACCACTACCTTTACTGTAGGCACCATCAATTCGTAT620960.12245770499872603No Hit
TATTCAATGAAGCGGAACTGTAGGCACCATCAATTCGTATGCCGTCTTCT619090.12208892776935923No Hit
TTTTTACTTATTCAATGAAGCGGAACTGGGCTCTGTAGGCACCATCAATT616950.12166690462986993No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC612920.12087216011952326Illumina Single End Adapter 1 (95% over 22bp)
TGTTGCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATT612800.12084849527057993No Hit
CTTGCTACGAGTCGCTGAGCGTCAGCCCGATCTGTAGGCACCATCAATTC611580.12060790263965612No Hit
AAATGGCCGGCAGAGCCCCGCACTGTAGGCACCATCAATTCGTATGCCGT587400.11583943557757614No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATTCGTA574340.11326391118424425No Hit
GGTTGTATAGTGTAGCGGTCAGCACGCCACTGTAGGCACCATCAATTCGT566260.11167047802206037No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG562580.1109447559877984Illumina Single End Adapter 1 (95% over 22bp)
TCGACGAGTCGAGTTGTTTGGGAATGCAGCTCCCTGTAGGCACCATCAAT554030.1092586355005865No Hit
TCTTGGTGATTCATAATAACTTGTCGAATCGCATCTGTAGGCACCATCAA553320.10911861847767183No Hit
TTTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGTAGGCACCATCAAT552790.10901409872817212No Hit
TTGGGGGCCTGAGTTGTAATTTGCCTGTAGGCACCATCAATTCGTATGCC544750.10742855384896935No Hit
CAATGAAGCGGAACTGGGCTTCTGTAGGCACCATCAATTCGTATGCCGTC538410.10617826099646369No Hit
CTTGGTGATTCATAATAACTTGTCGAATCGCCTGTAGGCACCATCAATTC523580.10325368008121776No Hit
AACTTGTCGAATCGCATCTGTAGGCACCATCAATTCGTATGCCGTCTTCT509880.10055194316018815No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGA509180.10041389820801874Illumina Single End Adapter 1 (95% over 22bp)
GTTTTCACGCCAGGTCGTACTCATAACCGCCTGTAGGCACCATCAATTCG509070.10039220542982069No Hit
ACCATCGACCGATCCTGACTGTAGGCACCATCAATTCGTATGCCGTCTTC507780.10013780830367996No Hit
GTTTTTTTACTTATTCAATGAAGCGGAACTGTAGGCACCATCAATTCGTA507320.10004709304939721No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)