FastQCFastQC Report
Mon 3 Jul 2023
SRR2016457.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2016457.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35352233
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTAGCGCAACTGGATCTCGTATGCCGTCTTCTG8271342.3396937896398224RNA PCR Primer, Index 23 (96% over 25bp)
AGGCTGAGGCGTGATGACGAGGCACATCTCGTATGC6852521.9383556337162635No Hit
AAGGCTGAGGCGTGATGACGAGGCACATCTCGTATG5089131.4395498015641615No Hit
ACGGTCCTAAGGTAGCGATCTCGTATGCCGTCTTCT3081780.871735598710271RNA PCR Primer, Index 38 (95% over 24bp)
AATGATAATTCAAGAATCATAACGGATCTCGTATGC2922070.82655882020239No Hit
AGGCTGAGGCGTGATGACGAGGCATCTCGTATGCCG2691580.7613606755759955No Hit
AGGTAGCGCCTCGTGAATCTCGTATGCCGTCTTCTG2455890.6946916196213122Illumina PCR Primer Index 8 (96% over 25bp)
ATTCATCTCCGGGGGTAGAGCATCTCGTATGCCGTC2035100.5756637777308155No Hit
ACGGTCCTAAGGTAGCGAATCTCGTATGCCGTCTTC1738870.49186991950409475RNA PCR Primer, Index 46 (100% over 21bp)
AGGGAGTATGCGATCTCGTATGCCGTCTTCTGCTTG1235060.3493584125223434RNA PCR Primer, Index 33 (96% over 29bp)
TGCTGAAGCAACAAATGCCCTGATCTCGTATGCCGT1160200.32818294674624937No Hit
AGGGAGTATGCGGATCTCGTATGCCGTCTTCTGCTT1131070.3199430146322016RNA PCR Primer, Index 23 (96% over 27bp)
TCGCAAACGACGAAAACTACGCATCTCGTATGCCGT1117130.3159998408021355No Hit
TGTGCGGGTTCAAGTCCCGCTCCGGGATCTCGTATG994150.2812127878881088No Hit
AGGCTGAGGCGTGATGACGAGGATCTCGTATGCCGT919290.2600373221120148No Hit
TGAGGCGAACCGGGGGAACTGAATCTCGTATGCCGT881300.24929118338861367No Hit
AGGCTGAGGCGTGATGACGAGGCAATCTCGTATGCC856770.24235244206497508No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGATCTCGTAT843710.2386581916904655No Hit
TGGTAGAGCGCACCCATCTCGTATGCCGTCTTCTGC838140.23708261936381783RNA PCR Primer, Index 14 (95% over 24bp)
TCGCAAACGACGAAAACTACGATCTCGTATGCCGTC834440.2360360093802278No Hit
AAGGCTGAGGCGTGATGACGAGGCATCTCGTATGCC820410.2320673774694798No Hit
AGGCTGAGGCGTGATGACGAGATCTCGTATGCCGTC809530.22898977838259893No Hit
TTGGATAGAGCAACGACATCTCGTATGCCGTCTTCT782640.2213834696099678RNA PCR Primer, Index 41 (96% over 26bp)
AGGAAAAGCCTCTAAGCATCTCGTATGCCGTCTTCT767230.21702448046209696RNA PCR Primer, Index 24 (95% over 23bp)
GCTTACGGGTTCAAGTCCCGTCCTCGGATCTCGTAT747720.2115057343053832No Hit
TAGGTAGCGCCTCGTGAATCTCGTATGCCGTCTTCT737940.20873928953794801RNA PCR Primer, Index 15 (95% over 23bp)
AGGCGTGTGACTGCGTACCATCTCGTATGCCGTCTT686230.19411220784836986RNA PCR Primer, Index 22 (95% over 23bp)
TCGGTAGAGCAGGGGATCTCGTATGCCGTCTTCTGC683350.19329754926654846RNA PCR Primer, Index 33 (96% over 27bp)
TGGTAGCGCAACTGGATCTCGTATGCCGTCTTCTGC678790.19200767317866455RNA PCR Primer, Index 23 (100% over 24bp)
AAGGCTGAGGCGTGATGACGAGGATCTCGTATGCCG665250.1881776463738514No Hit
TGAGGCGAACCGGGGGATCTCGTATGCCGTCTTCTG659530.18655964391273389RNA PCR Primer, Index 23 (95% over 24bp)
AGGCGAACCGGGGGATCTCGTATGCCGTCTTCTGCT648650.183482044825853RNA PCR Primer, Index 23 (96% over 26bp)
AAGGCTGAGGCGTGATGACGAGGCAATCTCGTATGC642840.18183858428405358No Hit
AGGTAGAGCAACTGATCTCGTATGCCGTCTTCTGCT589820.16684094608677194Illumina Paired End PCR Primer 2 (96% over 26bp)
ACGGTGCTGAAGCAACAAATATCTCGTATGCCGTCT582270.16470529598512207TruSeq Adapter, Index 6 (95% over 21bp)
TTGGTAGAGCGCACCCATCTCGTATGCCGTCTTCTG578680.16368980143347664Illumina Paired End PCR Primer 2 (95% over 24bp)
TAGCGCCTCGTGAATCTCGTATGCCGTCTTCTGCTT545450.15429011231058587RNA PCR Primer, Index 15 (96% over 27bp)
AGGGTTCCTGTCCATCTCGTATGCCGTCTTCTGCTT543530.1537470065893716RNA PCR Primer, Index 15 (96% over 28bp)
TCATGACTGGGGTGATCTCGTATGCCGTCTTCTGCT529790.14986040627193195Illumina PCR Primer Index 5 (96% over 26bp)
GCATCCGTAGCTCAGCTGGATCTCGTATGCCGTCTT519430.14692989831787995RNA PCR Primer, Index 23 (95% over 21bp)
TCTAAGTACCCCGAGGAAAAGAAATCTCGTATGCCG517840.14648013889249936No Hit
AACGGTCCTAAGGTAGCGATCTCGTATGCCGTCTTC488230.13810443034814804RNA PCR Primer, Index 38 (95% over 23bp)
AGAGATCTGGAGGAATCTCGTATGCCGTCTTCTGCT487080.13777913265054573Illumina PCR Primer Index 3 (96% over 26bp)
AGGGTGCAAGCGTTAATCGGATCTCGTATGCCGTCT486240.13754152389751448Illumina PCR Primer Index 9 (95% over 21bp)
AAGGCTGAGGCGTGATGACGAGATCTCGTATGCCGT463000.13096768173031673No Hit
TGCAAGTCGAACGGTAACAGATCTCGTATGCCGTCT462490.1308234192731192Illumina PCR Primer Index 9 (95% over 21bp)
ATTCATGACTGGGGTGATCTCGTATGCCGTCTTCTG458650.12973720783069062Illumina PCR Primer Index 5 (100% over 23bp)
CGGCGAGTAGCGCAGATCTCGTATGCCGTCTTCTGC454930.12868494049583798Illumina PCR Primer Index 9 (100% over 24bp)
GCTGATATGGCTCAGATCTCGTATGCCGTCTTCTGC453540.1282917545830839Illumina PCR Primer Index 9 (100% over 25bp)
AGGCGTGTGACTGCGTACATCTCGTATGCCGTCTTC448760.12693964763131085RNA PCR Primer, Index 16 (95% over 23bp)
AACCGTGAGGCTTAACATCTCGTATGCCGTCTTCTG445080.1258986949989835RNA PCR Primer, Index 24 (95% over 24bp)
ATCTCCGGGGGTAGAGCATCTCGTATGCCGTCTTCT440630.12463993434304418RNA PCR Primer, Index 43 (96% over 25bp)
ATAGAGTACTCGGATCTCGTATGCCGTCTTCTGCTT429180.12140110074517782RNA PCR Primer, Index 30 (100% over 26bp)
CGGAGTGGACGCATCACTGGATCTCGTATGCCGTCT424540.12008859525224334RNA PCR Primer, Index 30 (95% over 22bp)
ACGATGTCGACTTGGAGGTTGTGCCATCTCGTATGC417890.11820752595741264No Hit
TCTAAGTACTGAAGCAACAGCTATCTCGTATGCCGT414400.11722031816208045No Hit
AGCAGTGGGAGCACGCATCTCGTATGCCGTCTTCTG412720.11674510065601797TruSeq Adapter, Index 18 (95% over 24bp)
TGCTGAAGCAACAAATGCCCTGCATCTCGTATGCCG407650.11531096211093654No Hit
TGTCACTGGTTCGAGTCCAGTCAGAGGATCTCGTAT403430.11411726099451766No Hit
TCGGTTAGAATACCTGCATCTCGTATGCCGTCTTCT392750.11109623542026328Illumina PCR Primer Index 2 (95% over 22bp)
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGATCTCG384600.10879086478073394No Hit
TGAGGCGAACCGGGGGAACTGATCTCGTATGCCGTC381400.10788568857871014No Hit
GCATCCGCAGCTCAGCTGGATCTCGTATGCCGTCTT378330.10701728515989357RNA PCR Primer, Index 23 (95% over 21bp)
AGGCACGCTGATATGTAGGTGAAGCGACATCTCGTA375820.10630728757643118No Hit
GGAGCGGTAGTTCAGTCGGATCTCGTATGCCGTCTT373750.10572175172074702Illumina PCR Primer Index 9 (95% over 21bp)
ACCATGCACCGAAGCTGCGGCAATCTCGTATGCCGT373160.1055548598584989No Hit
AGGCGTGGCTTCCGATCTCGTATGCCGTCTTCTGCT366090.1035549861871526Illumina Paired End PCR Primer 2 (96% over 26bp)
TGCCCGGTAGCTAAATGCGGAAGATCTCGTATGCCG365050.10326080392149485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)