FastQCFastQC Report
Sun 2 Jul 2023
SRR2016459.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2016459.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35362647
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTGAGGCGTGATGACGAGGCACATCTCGTATGC12592733.5610258474146463No Hit
AAGGCTGAGGCGTGATGACGAGGCACATCTCGTATG11730813.3172884371466878No Hit
ATTCATCTCCGGGGGTAGAGCATCTCGTATGCCGTC3474860.9826357172866612No Hit
AGGCTGAGGCGTGATGACGAGGCATCTCGTATGCCG2584390.7308248163662635No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGATCTCG2490350.7042317844588952No Hit
AGGCACGCTGATATGTAGGTGAAGCGACATCTCGTA2160150.6108564214664134No Hit
AATGATAATTCAAGAATCATAACGGATCTCGTATGC2023530.5722224357243394No Hit
ACGATGTCGACTTGGAGGTTGTGCCATCTCGTATGC1930700.5459715727728187No Hit
TGCCCGGTAGCTAAATGCGGAAGATCTCGTATGCCG1751850.49539560768739965No Hit
TGAGGCGAACCGGGGGAACTGAATCTCGTATGCCGT1608720.4549206964060128No Hit
AGGCTGAGGCGTGATGACGAGGCAATCTCGTATGCC1408900.3984147453667708No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGATCTCGTAT1408380.3982676975510345No Hit
ACGGTCCTAAGGTAGCGATCTCGTATGCCGTCTTCT1395400.39459715784285043RNA PCR Primer, Index 38 (95% over 24bp)
AGGTAGCGCCTCGTGAATCTCGTATGCCGTCTTCTG1361750.3850814674591526Illumina PCR Primer Index 8 (96% over 25bp)
TTGGTAGCGCAACTGGATCTCGTATGCCGTCTTCTG1336050.37781391195065234RNA PCR Primer, Index 23 (96% over 25bp)
TCCCCATGCGAGAGTAGGGAACTGCCAGGCAATCTC1286790.36388395925225847No Hit
AAGGCTGAGGCGTGATGACGAGGCAATCTCGTATGC1263810.35738557693376294No Hit
AGGCGTGTGACTGCGTACCATCTCGTATGCCGTCTT1155150.32665823913011943RNA PCR Primer, Index 22 (95% over 23bp)
TGCTGAAGCAACAAATGCCCTGATCTCGTATGCCGT1136450.3213701734488371No Hit
AAGGCTGAGGCGTGATGACGAGGCATCTCGTATGCC1108010.31332778906511155No Hit
AGGCGAACCGGGGGATCTCGTATGCCGTCTTCTGCT1052460.2976191233648318RNA PCR Primer, Index 23 (96% over 26bp)
TGCGTAGAGATCTGGAGGATCTCGTATGCCGTCTTC1038150.2935724805894762RNA PCR Primer, Index 23 (95% over 22bp)
TGAGGCGAACCGGGGGATCTCGTATGCCGTCTTCTG946370.26761854111203837RNA PCR Primer, Index 23 (95% over 24bp)
TTGCACAATGGGCGCAAGCCTGATGCAGCATCTCGT930220.2630515752963855No Hit
GCTTACGGGTTCAAGTCCCGTCCTCGGATCTCGTAT896690.2535698190240114No Hit
TGTGCGGGTTCAAGTCCCGCTCCGGGATCTCGTATG878360.24838638351931064No Hit
ACGATGTCGACTTGGAGGTTGTGCATCTCGTATGCC860330.24328778329291922No Hit
GGGGCTACAGCTCAGCTGGGAGAGCGCCTGCATCTC802110.22682408361568632No Hit
TGAGGCGAACCGGGGGAACTGATCTCGTATGCCGTC797410.22549499758884No Hit
TGTCACTGGTTCGAGTCCAGTCAGAGGATCTCGTAT787590.22271805614551424No Hit
AAGCGGGTGAAAAGCCCGCTCGCCGGATCTCGTATG762750.21569369510150074No Hit
TCTAAGTACCCCGAGGAAAAGAAATCTCGTATGCCG755770.2137198609595034No Hit
ACAGCACTGTGCAAACACGATCTCGTATGCCGTCTT736800.2083554435277427Illumina PCR Primer Index 1 (100% over 21bp)
AGGCGAACCGGGGGAACTGATCTCGTATGCCGTCTT732420.2071168484644263Illumina PCR Primer Index 5 (95% over 21bp)
TCGCAAACGACGAAAACTACGCATCTCGTATGCCGT729260.20622325019956791No Hit
ACCGGAGTGGACGCATCACTGGTGATCTCGTATGCC728310.20595460515158834No Hit
AGGCGAACCGGGGGAACTGAATCTCGTATGCCGTCT726080.2053239962494889RNA PCR Primer, Index 32 (95% over 21bp)
AGGCTGAGGCGTGATGACGAGGATCTCGTATGCCGT711100.20108788801924243No Hit
AGGCGTGTGACTGCGTACCTTATCTCGTATGCCGTC698410.19749935574675728Illumina PCR Primer Index 10 (95% over 21bp)
AGTGGACGTATACGGTGATCTCGTATGCCGTCTTCT691790.19562732393873117Illumina PCR Primer Index 5 (100% over 22bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATCTC687020.19427844301361266No Hit
CGATGTCGACTTGGAGGTTGTGCCATCTCGTATGCC617860.17472108352069912No Hit
AAGGCTGAGGCGTGATGACGAGGATCTCGTATGCCG615320.17400281149768002No Hit
ACGTCCGTCGTGAAGAGGGAAACATCTCGTATGCCG606070.171387057083142No Hit
ACGGTGCTGAAGCAACAAATGCCCTGATCTCGTATG597650.16900601360525982No Hit
TCCCCATGCGAGAGTAGGGAACTGCCAGGCATATCT597650.16900601360525982No Hit
TGGGCGTTAAGTTGCAGGATCTCGTATGCCGTCTTC592030.1674167660582648RNA PCR Primer, Index 43 (95% over 22bp)
GGGGCTGATTCTGGATTCGACGGATCTCGTATGCCG585770.16564653658420989No Hit
TATCCTGTCTGAATATGGGGGGAATCTCGTATGCCG580010.16401769924067053No Hit
GGCTGAGGCGTGATGACGAGGCACATCTCGTATGCC578080.16347192561688043No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCATCTC572050.1617667365228627No Hit
ACCATGCACCGAAGCTGCGGCAGCGACGCATCTCGT571470.1616027216514646No Hit
AGGCTGAGGCGTGATGACGAGGCACATTTCGTATGC568840.1608589990449527No Hit
AGTCGAACGGTAACAGGAAACAGCATCTCGTATGCC561080.15866459317935108No Hit
ATCTCCGGGGGTAGAGCATCTCGTATGCCGTCTTCT554470.15679538921393527RNA PCR Primer, Index 43 (96% over 25bp)
AGGCGTGTGACTGCGTACATCTCGTATGCCGTCTTC544610.15400713640016825RNA PCR Primer, Index 16 (95% over 23bp)
AGTGGACGTATACGGTGTGATCTCGTATGCCGTCTT537830.15208985911037712Illumina PCR Primer Index 5 (95% over 21bp)
AAGCCAGCTTGACTGCGAGCGATCTCGTATGCCGTC532470.1505741354712502No Hit
ACCATGCACCGAAGCTGCGGCAATCTCGTATGCCGT525910.14871907071888596No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGATCTCGTA525240.14852960526399508No Hit
ACGGTCCTAAGGTAGCGAATCTCGTATGCCGTCTTC519950.14703367652314037RNA PCR Primer, Index 46 (100% over 21bp)
TAAGTACCCCGAGGAAAAGAAATCTCGTATGCCGTC516200.1459732355442736No Hit
AGGGTTCCTGTCCATCTCGTATGCCGTCTTCTGCTT503790.14246388286487718RNA PCR Primer, Index 15 (96% over 28bp)
ATGCGTAGAGATCTGGAGGATCTCGTATGCCGTCTT496990.14054094988986543RNA PCR Primer, Index 47 (95% over 21bp)
TGCACAATGGGCGCAAGCCTGATGCAGCATCTCGTA491960.13911854505687882No Hit
ACCATGCACCGAAGCTGCGGCAGATCTCGTATGCCG477430.13500968974409636No Hit
AACGGTCCTAAGGTAGCGATCTCGTATGCCGTCTTC471750.1334034751414395RNA PCR Primer, Index 38 (95% over 23bp)
AACGATGTCGACTTGGAGGTTGTGCCATCTCGTATG461810.13059259958678998No Hit
TCGGACGCGGGTTCAACTCCCGCCAGCTCCATCTCG460280.13015993966741232No Hit
TCACTGGTTCGAGTCCAGTCAGAGGATCTCGTATGC451400.12764881542945583No Hit
AGGCGTGTGACTGCGTACCTTTTGATCTCGTATGCC451320.12762619268857334No Hit
AGGCGTGGCTTCCGGATCTCGTATGCCGTCTTCTGC440640.12460605678076078RNA PCR Primer, Index 30 (100% over 25bp)
ACCGGAGTGGACGCATCACTGGATCTCGTATGCCGT440450.12455232777116487No Hit
ACTAACTGGAGGACCGAACCGACATCTCGTATGCCG435450.12313840646600918No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGATCTCG431830.12211472744107646No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCATCT422340.11943110480389094No Hit
AGGCGTGTGACTGCGTACCTATCTCGTATGCCGTCT417130.11795779880391871RNA PCR Primer, Index 27 (95% over 21bp)
TGGGCGTTAAGTTGCAGGGATCTCGTATGCCGTCTT415680.11754776162542356Illumina PCR Primer Index 5 (95% over 22bp)
ACTAACTGGAGGACCGATCTCGTATGCCGTCTTCTG408860.1156191729651912Illumina Paired End PCR Primer 2 (100% over 25bp)
ACGGTGCTGAAGCAACAAATATCTCGTATGCCGTCT393280.11121339417832607TruSeq Adapter, Index 6 (95% over 21bp)
ATGAGGCGAACCGGGGGATCTCGTATGCCGTCTTCT391970.11084294679637528RNA PCR Primer, Index 23 (95% over 23bp)
TGCCCGGTAGCTAAATGCGGATCTCGTATGCCGTCT387530.10958738467739702No Hit
AAGGCACGCTGATATGTAGGTGAAGCGACATCTCGT374710.10596209045097783No Hit
CACGCTGATATGTAGGTGAAGCGACATCTCGTATGC370690.10482529772163267No Hit
TAGGTAGCGCCTCGTGAATCTCGTATGCCGTCTTCT369750.1045594805162634RNA PCR Primer, Index 15 (95% over 23bp)
TCGCAAACGACGAAAACTACGATCTCGTATGCCGTC364280.10301265060842306No Hit
AGCAGTGGGAGCACGCATCTCGTATGCCGTCTTCTG362820.1025997855873176TruSeq Adapter, Index 18 (95% over 24bp)
AGGGTGCAAGCGTTAATCGGATCTCGTATGCCGTCT362300.1024527377715814Illumina PCR Primer Index 9 (95% over 21bp)
TCCTGTCTGAATATGGGGGGAATCTCGTATGCCGTC358960.1015082383397374No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)