FastQCFastQC Report
Mon 3 Jul 2023
SRR2016461.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2016461.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31494400
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTGAGGCGTGATGACGAGATCTCGTATGCCGTC19344636.142244335500915No Hit
AGGCTGAGGCGTGATGACGAGGCATCTCGTATGCCG13901574.413981533224955No Hit
TGCTGAAGCAACAAATGCCCTGATCTCGTATGCCGT4359011.3840587533021742No Hit
AGGCTGAGGCGTGATGACGAGGATCTCGTATGCCGT3737691.1867792369437107No Hit
AGGCTGAGGCGTGATGACGATCTCGTATGCCGTCTT2465620.7828756858362121Illumina PCR Primer Index 1 (95% over 23bp)
GGCTGAGGCGTGATGACGAGATCTCGTATGCCGTCT2257220.7167051920341394RNA PCR Primer, Index 31 (95% over 21bp)
TGAGGCGAACCGGGGGAACTGATCTCGTATGCCGTC2256660.7165273826458037No Hit
AGGCTGAGGCGTGATGACGAGGCACATCTCGTATGC2109570.6698238416988417No Hit
ACTGCCAGGCAATCTCGTATGCCGTCTTCTGCTTGA2104480.6682076813655761RNA PCR Primer, Index 33 (96% over 31bp)
TAAGTACCCCGAGGAAAAGAATCTCGTATGCCGTCT1870720.5939849624060151No Hit
AAGGCTGAGGCGTGATGACGAGATCTCGTATGCCGT1568540.4980377463930095No Hit
AGCACGCCTGGAAAGTGTGATCTCGTATGCCGTCTT1540830.48923935683804104Illumina PCR Primer Index 5 (95% over 21bp)
ACGGTCCTAAGGTAGCGATCTCGTATGCCGTCTTCT1521290.4830350792521845RNA PCR Primer, Index 38 (95% over 24bp)
ATTCATCTCCGGGGGTAGAGCATCTCGTATGCCGTC1504330.4776499949197318No Hit
TTGGTAGCGCAACTGGATCTCGTATGCCGTCTTCTG1474600.4682102214996952RNA PCR Primer, Index 23 (96% over 25bp)
AGGTAGCGCCTCGTGAATCTCGTATGCCGTCTTCTG1432830.454947546230441Illumina PCR Primer Index 8 (96% over 25bp)
AGGCGAACCGGGGGAACTGATCTCGTATGCCGTCTT1369750.4349185887014834Illumina PCR Primer Index 5 (95% over 21bp)
TAAGTACCCCGAGGAAAAGAAATCTCGTATGCCGTC1369530.4348487350132087No Hit
TGCTGAAGCAACAAATGCCCTGCATCTCGTATGCCG1320370.419239610851453No Hit
AGGCTGAGGATCTCGTATGCCGTCTTCTGCTTGAAA1130640.35899715504978663RNA PCR Primer, Index 26 (96% over 29bp)
TGAGGCGAACCGGGGGAACTGAATCTCGTATGCCGT1057590.3358025553749238No Hit
CTGAAGCAACAAATGCCCTGATCTCGTATGCCGTCT1051900.3339958849827271No Hit
AGGCTGAGGCGTGATGACGAATCTCGTATGCCGTCT1035740.3288648140621825Illumina PCR Primer Index 4 (95% over 22bp)
CGAGCGAACGGGGAGCAGCATCTCGTATGCCGTCTT1027420.32622307457833777RNA PCR Primer, Index 24 (95% over 22bp)
CGTGATGACGAGATCTCGTATGCCGTCTTCTGCTTG991590.3148464488925015RNA PCR Primer, Index 31 (96% over 29bp)
TCTAAGTACCCCGAGGAAAAGAAATCTCGTATGCCG928390.2947793893517578No Hit
ACGGTCCTAAGGTAGCGAATCTCGTATGCCGTCTTC878380.2789003759398496RNA PCR Primer, Index 46 (100% over 21bp)
TCTAAGTACCCCGAGGAAAAGAATCTCGTATGCCGT866790.27522035663483035No Hit
TCCGGTTCGACTCCGGAACGCGCCTCCAATCTCGTA856840.2720610648242227No Hit
AAGGCTGAGGCGTGATGACGAGGCATCTCGTATGCC846560.268796992481203No Hit
TGAAGCAACAAATGCCCTGATCTCGTATGCCGTCTT822110.2610337075797602No Hit
TGAGGCGAACCGGGGGAACTATCTCGTATGCCGTCT802150.2546960729526519No Hit
ACACACGGCGGGTGATCTCGTATGCCGTCTTCTGCT687900.21841978256451944RNA PCR Primer, Index 33 (96% over 26bp)
ACGGGGCTAATCTCGTATGCCGTCTTCTGCTTGAAA668450.21224408148750254RNA PCR Primer, Index 29 (100% over 27bp)
TAGGTAGCGCCTCGTGAATCTCGTATGCCGTCTTCT588850.18696974700264174RNA PCR Primer, Index 15 (95% over 23bp)
AAGAACCTTACCTGGATCTCGTATGCCGTCTTCTGC565990.1797113137573664RNA PCR Primer, Index 23 (100% over 24bp)
GGCTGAGGCGTGATGACGAGGCATCTCGTATGCCGT563510.1789238721804511No Hit
TGAGGCGAACCGGGGGAACATCTCGTATGCCGTCTT536110.17022391282259702RNA PCR Primer, Index 37 (95% over 22bp)
TGCTGAAGCAACAAATGCCCTATCTCGTATGCCGTC534740.16978891485470432No Hit
AAGGCTGAGGCGTGATGACGAGGATCTCGTATGCCG507940.16127946555578135No Hit
AGGCGAACCGGGGGAACTGAATCTCGTATGCCGTCT504480.1601808575492786RNA PCR Primer, Index 32 (95% over 21bp)
TAGCGCCTCGTGAATCTCGTATGCCGTCTTCTGCTT498820.15838371266002846RNA PCR Primer, Index 15 (96% over 27bp)
AGGCGTGTGACTGCGTACCATCTCGTATGCCGTCTT494600.15704379191221296RNA PCR Primer, Index 22 (95% over 23bp)
TGCTGAAGCAACAAATGCCCATCTCGTATGCCGTCT465970.1479532869335501RNA PCR Primer, Index 20 (95% over 21bp)
TAAGTACCCCGAGGAAAAGATCTCGTATGCCGTCTT445320.14139656573867102RNA PCR Primer, Index 28 (95% over 23bp)
TTAGGTAGCGCCTCGTGAATCTCGTATGCCGTCTTC445270.14138068990042674Illumina PCR Primer Index 8 (95% over 23bp)
AGGCGAACCGGGGGAACTATCTCGTATGCCGTCTTC417590.1325918258484048RNA PCR Primer, Index 36 (95% over 22bp)
ATCTCCGGGGGTAGAGCATCTCGTATGCCGTCTTCT404820.12853713676082096RNA PCR Primer, Index 43 (96% over 25bp)
ATCAAATCGTACCCCAAACCGATCTCGTATGCCGTC404590.12846410790489737Illumina Paired End PCR Primer 2 (95% over 21bp)
GATGACGAGATCTCGTATGCCGTCTTCTGCTTGAAA400500.12716546433651696RNA PCR Primer, Index 41 (96% over 30bp)
AGGCGTGTGACTGCGTACCTTATCTCGTATGCCGTC398510.12653360597439545Illumina PCR Primer Index 10 (95% over 21bp)
GGCTGAGGCGTGATGACGAGGATCTCGTATGCCGTC390940.1241300040642146No Hit
AAGTACCCCGAGGAAAAGAATCTCGTATGCCGTCTT389300.12360927656980289RNA PCR Primer, Index 36 (95% over 21bp)
AGAGTACTCGGATCTCGTATGCCGTCTTCTGCTTGA385090.12227253098963625RNA PCR Primer, Index 30 (100% over 27bp)
ACACGGCGGGTGATCTCGTATGCCGTCTTCTGCTTG382450.12143428673033936Illumina PCR Primer Index 5 (96% over 28bp)
CTGAAGCAACAAATGCCCTGCATCTCGTATGCCGTC371800.11805273318431214No Hit
AGGCGTGTGACTGCGTACCTTTATCTCGTATGCCGT365160.1159444218654745No Hit
AGGTTGAGGCGTGATGACGAGATCTCGTATGCCGTC362640.11514427961796382No Hit
GTGATGACGAGATCTCGTATGCCGTCTTCTGCTTGA358230.11374403068482015RNA PCR Primer, Index 41 (96% over 30bp)
AGGCTGAGGCGTGATGATGAGATCTCGTATGCCGTC353760.11232473074578336No Hit
AGGCTGAGGCGTGATGACGAGATTTCGTATGCCGTC348150.11054346169477747No Hit
ATGTAGGTGAAGCGACATCTCGTATGCCGTCTTCTG346900.110146565738671Illumina PCR Primer Index 11 (96% over 25bp)
AAGGCTGAGGCGTGATGACGAGGCACATCTCGTATG346120.10989890266206055No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)