FastQCFastQC Report
Sat 1 Jul 2023
SRR2016463.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2016463.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32997315
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTGAGGCGTGATGACGAGGCATCTCGTATGCCG17141305.194755997571318No Hit
AGGCTGAGGCGTGATGACGAGATCTCGTATGCCGTC7319382.2181744181306873No Hit
GGGGCTACAGCTCAGCTGGGAGAGCGCCTGCATCTC6086171.8444440100656674No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATCTC5783431.752697151268217No Hit
AGGCTGAGGCGTGATGACGAGGATCTCGTATGCCGT4411071.3367966454240292No Hit
AGGCTGAGGCGTGATGACGAGGCACATCTCGTATGC3747751.1357742289031698No Hit
AATGATAATTCAAGAATCATAACGGATCTCGTATGC3457451.0477973738166273No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGATCTCG3049320.9241115527126981No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGATCTCGTA3031860.9188202130991567No Hit
AGGCACGCTGATATGTAGGTGAAGCGACATCTCGTA2775510.8411320739278333No Hit
TGTGCGGGTTCAAGTCCCGCTCCGGGATCTCGTATG2338560.7087122088569934No Hit
AAGGCTGAGGCGTGATGACGAGGCATCTCGTATGCC2306180.698899289230048No Hit
ATTCATCTCCGGGGGTAGAGCATCTCGTATGCCGTC2043080.619165529074108No Hit
ACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATCTC1840810.557866602176571No Hit
GCTTACGGGTTCAAGTCCCGTCCTCGGATCTCGTAT1833670.5557027897572878No Hit
GGGGCTAAAGCTCAGCTGGGAGAGCGCCTGCATCTC1743720.5284429960437691No Hit
AAGGCTGAGGCGTGATGACGAGATCTCGTATGCCGT1534860.465146936955325No Hit
TCCCCATGCGAGAGTAGGGAACTGCCAGGCAATCTC1514780.4590615933447918No Hit
AAGGCTGAGGCGTGATGACGAGGCACATCTCGTATG1352040.4097424290430903No Hit
TGCTGAAGCAACAAATGCCCTGATCTCGTATGCCGT1345580.4077846939970721No Hit
TGAGGCGAACCGGGGGAACTGATCTCGTATGCCGTC1322350.4007447272603847No Hit
TTGGTAGCGCAACTGGATCTCGTATGCCGTCTTCTG1260170.3819007698050584RNA PCR Primer, Index 23 (96% over 25bp)
TGAGGCGAACCGGGGGAACTGAATCTCGTATGCCGT1239040.37549721848580714No Hit
ACGTGCCGGTTCGAGTCCGGCCTTCGGATCTCGTAT1198560.36322955367732196No Hit
AAGGCTGAGGCGTGATGACGAGGATCTCGTATGCCG1155520.35018606816948594No Hit
AGGGAACTGCCAGGCAATCTCGTATGCCGTCTTCTG1108410.33590914897166635RNA PCR Primer, Index 33 (96% over 27bp)
ACCATGCACCGAAGCTGCGGCAGCGACGCATCTCGT1103390.33438781306903304No Hit
GCCCGGACGGTGGAATCGGTAGACACATCTCGTATG1006800.30511573441657297No Hit
GCCCGGATGGTGGAATCGGTAGACACATCTCGTATG975330.29557859480384996No Hit
TCGGACGCGGGTTCAACTCCCGCCAGCTCCATCTCG941980.2854717118650411No Hit
CGAATCCTCCCGGATCTCGTATGCCGTCTTCTGCTT915000.2772952890257889RNA PCR Primer, Index 30 (96% over 28bp)
ACGATGTCGACTTGGAGGTTGTGCATCTCGTATGCC863490.26168492800096005No Hit
ACGGTCCTAAGGTAGCGATCTCGTATGCCGTCTTCT775960.23515852729229636RNA PCR Primer, Index 38 (95% over 24bp)
ACCATGCACCGAAGCTGCGGCAATCTCGTATGCCGT775430.23499790816313387No Hit
ACCGAGCGGCGGAGGTTCGAATCCTCCCGGATCTCG725130.2197542436407326No Hit
TAAGTACCCCGAGGAAAAGAAATCTCGTATGCCGTC717590.2174692092371758No Hit
TGCAAGTCGAACGGTAACAGATCTCGTATGCCGTCT713500.2162297144479786Illumina PCR Primer Index 9 (95% over 21bp)
GGGGCTGATTCTGGATTCGACGGATCTCGTATGCCG712150.21582059025105527No Hit
AGGCGTGTGACTGCGTACCTTATCTCGTATGCCGTC708090.21459018711067857Illumina PCR Primer Index 10 (95% over 21bp)
TCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCATCT679670.2059773651280415No Hit
CTTACGGGTTCAAGTCCCGTCCTCGGATCTCGTATG633020.1918398512121365No Hit
AGGCTGAGGCGTGATGACGAGGCAATCTCGTATGCC589530.17865999097199273No Hit
GGCTGAGGCGTGATGACGAGGCATCTCGTATGCCGT581980.1763719260188291No Hit
GGAGCGGTAGTTCAGTCGGATCTCGTATGCCGTCTT577810.17510818683277715Illumina PCR Primer Index 9 (95% over 21bp)
GGCTGAGGCGTGATGACGAGATCTCGTATGCCGTCT577270.17494453715400782RNA PCR Primer, Index 31 (95% over 21bp)
TGCTGAAGCAACAAATGCCCTGCATCTCGTATGCCG574290.17404143337116973No Hit
AGGCGAACCGGGGGAACTGATCTCGTATGCCGTCTT571960.17333531531277618Illumina PCR Primer Index 5 (95% over 21bp)
GCGGGAACAGCTCAGTTGGTAGAGCACGATCTCGTA536530.16259807805574483No Hit
TGCCCGGTAGCTAAATGCGGAAGATCTCGTATGCCG532390.1613434305185134No Hit
AGGCGTGTGACTGCGTACCTTTTGATCTCGTATGCC526620.15959480339536716No Hit
AGGTAGCGCCTCGTGAATCTCGTATGCCGTCTTCTG518410.15710672216815216Illumina PCR Primer Index 8 (96% over 25bp)
TGTCACTGGTTCGAGTCCAGTCAGAGGATCTCGTAT509700.15446711346059522No Hit
AGCAGGTGCGAAAGCAGGTCATAGATCTCGTATGCC507230.1537185677077059No Hit
ACCGAGCGGAGGTTCGAATCCTCCCGGATCTCGTAT503520.15259423380356857No Hit
TCTAAGTACCCCGAGGAAAAGAAATCTCGTATGCCG491090.14882726064226742No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCATCTC485970.14727561924356572No Hit
TAAGTACCCCGAGGAAAAGAATCTCGTATGCCGTCT480320.14556335871570159No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACATCTCG478940.14514514286995775No Hit
CGGTAGTTCAGTCGGTTAGAATACCTGCATCTCGTA478510.14501482923686368No Hit
ACCATGCACCGAAGCTGCGGCAGATCTCGTATGCCG463280.14039930218564753No Hit
GGGGCTACAGCTCAGCTGGGAGAGCGCTTGCATCTC459790.13934164037286065No Hit
AGGCGTGTGACTGCGTACCTTTATCTCGTATGCCGT459100.13913253244998874No Hit
ATGCAAGTCGAACGGTAACAGATCTCGTATGCCGTC459050.13911737970195454No Hit
AGGAAAAGCCTCTAAGCATCTCGTATGCCGTCTTCT438680.13294415015282304RNA PCR Primer, Index 24 (95% over 23bp)
GCGAAGGTGGCGGAATTGGTAGACGCGATCTCGTAT425610.12898322181668417No Hit
ACGGTCCTAAGGTAGCGAATCTCGTATGCCGTCTTC418180.12673152345880262RNA PCR Primer, Index 46 (100% over 21bp)
GCGCTGTGCGGGTTCAAGTCCCGCTCCGGGATCTCG415380.12588296956888764No Hit
TCCGTAGCTCAGCTGGATAGAGTACTCGGATCTCGT403470.12227358498714212No Hit
ACGCTGATATGTAGGTGAAGCGACATCTCGTATGCC397400.12043404137579072No Hit
ATGTAGGTGAAGCGACATCTCGTATGCCGTCTTCTG396210.12007340597257686Illumina PCR Primer Index 11 (96% over 25bp)
ACCGAGCCGGAGGTTCGAATCCTCCCGGATCTCGTA389890.11815809862105446No Hit
ACACATGCAAGTCGAACGGTAATCTCGTATGCCGTC387320.11737924737209679No Hit
ACGATGTCGACTTGGAGGTTGTGCCATCTCGTATGC376520.11410625379671042No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGATCTCG373100.11306980583117143No Hit
TCCGCAGCTCAGCTGGATAGAGTACTCGGATCTCGT365130.11065445779452054No Hit
GGGGCTACAGCTCAGCTGGGAGAGCGCCTGATCTCG360420.10922706892969927No Hit
TGCGTAGAGATCTGGAGGATCTCGTATGCCGTCTTC360340.10920282453284456RNA PCR Primer, Index 23 (95% over 22bp)
GGAGCGGCAGTTCAGTCGGATCTCGTATGCCGTCTT357170.10824214030747653Illumina PCR Primer Index 9 (95% over 21bp)
AAGTCGAACGGTAACAGATCTCGTATGCCGTCTTCT350820.10631774130713363RNA PCR Primer, Index 17 (95% over 23bp)
ACATGCAAGTCGAACGGTAATCTCGTATGCCGTCTT350670.10627228306303103RNA PCR Primer, Index 48 (95% over 22bp)
AGGCGTGTGACTGCGTACCTTTTGTATCTCGTATGC347440.10529341554002197No Hit
AGTCGGTTAGAATACCTGCATCTCGTATGCCGTCTT346310.10495096343444914No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCCTGATCTCG345810.10479943595410718No Hit
GGCTGAGGCGTGATGACGAGGATCTCGTATGCCGTC336520.10198405536935354No Hit
AGCTCAGTTGGTAGAGCACGATCTCGTATGCCGTCT335120.10155977842439604TruSeq Adapter, Index 1 (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)