FastQCFastQC Report
Sat 1 Jul 2023
SRR2016465.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2016465.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29770749
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTGAGGCGTGATGACGAGGCACATCTCGTATGC19734726.6288960348293555No Hit
AAGGCTGAGGCGTGATGACGAGGCACATCTCGTATG14984055.033145118384492No Hit
AGGCTGAGGCGTGATGACGAGGCATCTCGTATGCCG11401533.8297759992534957No Hit
AAGGCTGAGGCGTGATGACGAGGCATCTCGTATGCC4727831.5880789562936426No Hit
ACGGTCCTAAGGTAGCGATCTCGTATGCCGTCTTCT4029411.3534795513542504RNA PCR Primer, Index 38 (95% over 24bp)
ATTCATCTCCGGGGGTAGAGCATCTCGTATGCCGTC1843970.6193898581456583No Hit
TTGGTAGCGCAACTGGATCTCGTATGCCGTCTTCTG1775670.5964478757319811RNA PCR Primer, Index 23 (96% over 25bp)
AGGTAGCGCCTCGTGAATCTCGTATGCCGTCTTCTG1733620.5823232730892999Illumina PCR Primer Index 8 (96% over 25bp)
ACGGTCCTAAGGTAGCGAATCTCGTATGCCGTCTTC1695580.569545630175445RNA PCR Primer, Index 46 (100% over 21bp)
AGGCTGAGGCGTGATGACGAGGCAATCTCGTATGCC1638610.5504093968210205No Hit
ACCATGCACCGAAGCTGCGGCAATCTCGTATGCCGT1479410.49693408788606563No Hit
AAGGCTGAGGCGTGATGACGAGGCAATCTCGTATGC1401470.47075402772029684No Hit
AGGCGTGTGACTGCGTACCATCTCGTATGCCGTCTT1401260.4706834886821289RNA PCR Primer, Index 22 (95% over 23bp)
ACTGCCAGGCATATCTCGTATGCCGTCTTCTGCTTG1073320.36052838307830276RNA PCR Primer, Index 37 (96% over 29bp)
AGGCGTGTGACTGCGTACATCTCGTATGCCGTCTTC946450.3179127270193975RNA PCR Primer, Index 16 (95% over 23bp)
AACGGTCCTAAGGTAGCGATCTCGTATGCCGTCTTC901650.3028643988768976RNA PCR Primer, Index 38 (95% over 23bp)
AAGAACCTTACCTGGTCATCTCGTATGCCGTCTTCT854820.28713419336544066RNA PCR Primer, Index 20 (95% over 24bp)
ACCATGCACCGAAGCTGCGGCAGATCTCGTATGCCG854470.2870166283018274No Hit
ATGTAGGTGAAGCGACATCTCGTATGCCGTCTTCTG852370.28631123792014773Illumina PCR Primer Index 11 (96% over 25bp)
TGAGGCGAACCGGGGGATCTCGTATGCCGTCTTCTG805920.2707086744777567RNA PCR Primer, Index 23 (95% over 24bp)
TAGGTAGCGCCTCGTGAATCTCGTATGCCGTCTTCT741630.24911365179290582RNA PCR Primer, Index 15 (95% over 23bp)
GGCTGAGGCGTGATGACGAGGCACATCTCGTATGCC734550.2467354785061001No Hit
AGGCTGAGGCGTGATGACGAGGATCTCGTATGCCGT725840.24380978792303815No Hit
TGAGGCGAACCGGGGGAACTGAATCTCGTATGCCGT700540.23531151332470673No Hit
ACGGTGCTGAAGCAACAAATATCTCGTATGCCGTCT679090.22810645442612143TruSeq Adapter, Index 6 (95% over 21bp)
AGGGAGTATGCGATCTCGTATGCCGTCTTCTGCTTG608290.2043247215580636RNA PCR Primer, Index 33 (96% over 29bp)
AAGGCTGAGGCGTGATGACGAGGATCTCGTATGCCG607640.20410638643992463No Hit
TGCGTAGAGATCTGGAGGATCTCGTATGCCGTCTTC585410.19663932539957257RNA PCR Primer, Index 23 (95% over 22bp)
TTGGATAGAGCAACGACATCTCGTATGCCGTCTTCT540010.18138945714802138RNA PCR Primer, Index 41 (96% over 26bp)
AGGCTGAGGCGTGATGACGAGATCTCGTATGCCGTC532520.17887356478669716No Hit
AGTGGACGTATACGGTGATCTCGTATGCCGTCTTCT526900.17698580576524964Illumina PCR Primer Index 5 (100% over 22bp)
AGCACGCCTGGAAAGTGTGATCTCGTATGCCGTCTT498170.1673353935435081Illumina PCR Primer Index 5 (95% over 21bp)
AGCAGTGGGAGCACGCATCTCGTATGCCGTCTTCTG476390.16001948758494453TruSeq Adapter, Index 18 (95% over 24bp)
AGGCTGAGGCGTGATGACGAGGCACATTTCGTATGC469390.15766818631267893No Hit
AGGCGAACCGGGGGATCTCGTATGCCGTCTTCTGCT468840.1574834412127152RNA PCR Primer, Index 23 (96% over 26bp)
AAGGCTGAGGATCTCGTATGCCGTCTTCTGCTTGAA463670.15574683727305616RNA PCR Primer, Index 23 (96% over 28bp)
TGCCCGGTAGCTAAATGCGGAAGATCTCGTATGCCG461960.15517244796225987No Hit
ACTAACTGGAGGACCGATCTCGTATGCCGTCTTCTG449200.1508863616431014Illumina Paired End PCR Primer 2 (100% over 25bp)
ACTGCCAGGCAATCTCGTATGCCGTCTTCTGCTTGA428570.14395674089355293RNA PCR Primer, Index 33 (96% over 31bp)
AACCGTGAGGCTTAACCATCTCGTATGCCGTCTTCT428020.1437719957935892Illumina PCR Primer Index 7 (95% over 22bp)
ATATGTAGGTGAAGCGACATCTCGTATGCCGTCTTC427060.14344953161910706RNA PCR Primer, Index 41 (100% over 22bp)
CAAGCAGTGGGAGCACGCATCTCGTATGCCGTCTTC422320.1418573647576015Illumina PCR Primer Index 3 (95% over 22bp)
CGGCGAGTAGCGCAGCATCTCGTATGCCGTCTTCTG405520.13621424170416405RNA PCR Primer, Index 24 (96% over 25bp)
GGCTGAGGCGTGATGACGAGGCATCTCGTATGCCGT405030.13604965061510546No Hit
TGCTGAAGCAACAAATGCCCTGATCTCGTATGCCGT400830.13463886985174608No Hit
ACCTTCGGGAGGGCGATCTCGTATGCCGTCTTCTGC400770.13461871584084095RNA PCR Primer, Index 33 (96% over 26bp)
TAGCGCCTCGTGAATCTCGTATGCCGTCTTCTGCTT392650.13189120636501286RNA PCR Primer, Index 15 (96% over 27bp)
AAGAACCTTACCTGGATCTCGTATGCCGTCTTCTGC389250.1307491457470553RNA PCR Primer, Index 23 (100% over 24bp)
AGGGAGTATGCGGATCTCGTATGCCGTCTTCTGCTT387280.13008742238900337RNA PCR Primer, Index 23 (96% over 27bp)
TCGCAAACGACGAAAACTACGCATCTCGTATGCCGT385970.12964739315090795No Hit
ATTCATGACTGGGGTGATCTCGTATGCCGTCTTCTG381960.12830043342208153Illumina PCR Primer Index 5 (100% over 23bp)
AACCGTGAGGCTTAACATCTCGTATGCCGTCTTCTG381360.12809889331303018RNA PCR Primer, Index 24 (95% over 24bp)
TATGTAGGTGAAGCGACATCTCGTATGCCGTCTTCT370340.12439727331012061RNA PCR Primer, Index 41 (100% over 23bp)
AGGCGTGTGACTGCGTACCTATCTCGTATGCCGTCT369910.12425283623196716RNA PCR Primer, Index 27 (95% over 21bp)
AGGCGTGTGACTGCGTACCTTATCTCGTATGCCGTC368110.12364821590481313Illumina PCR Primer Index 10 (95% over 21bp)
ACGGTGCTGAAGCAACAATCTCGTATGCCGTCTTCT350840.11784721976595214RNA PCR Primer, Index 36 (100% over 24bp)
ACGGTGCTGAAGCAACATCTCGTATGCCGTCTTCTG350660.11778675773323674Illumina PCR Primer Index 11 (96% over 25bp)
ACGGTGCTGAAGCAACAAATCTCGTATGCCGTCTTC343010.11521712134283218RNA PCR Primer, Index 29 (95% over 24bp)
ACCATGCACCGAAGCTGCGGCATCTCGTATGCCGTC341500.11470991206838632No Hit
ATGAGGCGAACCGGGGGATCTCGTATGCCGTCTTCT334060.11221081471614973RNA PCR Primer, Index 23 (95% over 23bp)
AATAGTCGCAAACGACGAAAACATCTCGTATGCCGT330030.11085713698368825No Hit
TGCTGAAGCAACAAATGCCCTGCATCTCGTATGCCG326600.10970499936027811No Hit
TACGGAGGGTGCAAGCGTTATCTCGTATGCCGTCTT322090.10819008954057555RNA PCR Primer, Index 31 (95% over 21bp)
ACACACGGCGGGTGATCTCGTATGCCGTCTTCTGCT313790.10540211803203206RNA PCR Primer, Index 33 (96% over 26bp)
TACGGAGGGTGCAAGCGTATCTCGTATGCCGTCTTC306160.10283919964526253RNA PCR Primer, Index 27 (95% over 21bp)
ACAAGCAGTGGGAGCACGCATCTCGTATGCCGTCTT300630.1009816716401727TruSeq Adapter, Index 18 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)