FastQCFastQC Report
Sat 1 Jul 2023
SRR2033089.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2033089.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89928275
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA985695710.960909680520391No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA40337214.485486906092661No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT33932713.7733082281407047No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT32985313.667957602878516No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA28884083.211901929621134No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA27233473.0283545414387185No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT23668862.6319708678944416No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATATC20735872.3058231685195785No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT11506221.2794885701966372No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT9487171.054970753080719No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT9053391.0067345337158975No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATA8289910.9218357630011251No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA7794460.8667418562181917No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA7148560.7949179498883971No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT5225040.5810230430862818No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG5050110.5615708741216263No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT4874060.5419941614581176No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT4699800.522616496313312No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA4588610.5102521982101847No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAA4570410.508228363103818No Hit
TGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT4272450.4750952912195858No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA4231370.47052720626521527No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATA4209410.4680852601698409No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA3928790.436880391623213No Hit
CAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA3768000.419000586856581No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT3767770.4189750109184236No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAT3399700.37804572588543484No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3161490.35155683793556586No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATATCTC3152280.35053268841195945No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA2992510.33276630737106877No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG2941860.3271340409898889No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATATCT2486470.27649479543558464No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATATCTCGT2327860.2588574060827921No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAAT2310420.25691808277207584No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTCG2277710.25328073956717173No Hit
GGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT2181100.2425377335437603No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAA2180840.24250882161366932No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATATCT2101520.23368845894130627No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA2089700.2323740781194791No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA2072270.23043586680607406No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAT1965060.2185141436328007No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1849330.20564499875039302No Hit
GGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATATCTCG1785700.19856935985928786No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCCTGTAGGCACCATCAATAT1698080.18882603941863668No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC1563760.17388969153472586No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC1509180.16782041021024813No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAAT1447960.16101276267113984No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1428570.1588565998847415No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAT1424700.15842625692531073No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC1421190.1580359458690829No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCGT1371480.1525082072351549No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATATCTCGT1327140.14757761115733622No Hit
TGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATATC1311600.14584956733574617No Hit
TCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATATC1309220.14558491197568285No Hit
CTCCCGGGGCTCCGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT1295010.14400476379648114No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTCGT1291740.14364114067572184No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCCTGTAGGCACCATCAAT1248470.13882952831020054No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1198490.13327176574887042No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATATCTC1190430.13237549591605088No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATATCT1183900.13164936167184346No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAA1134750.12618389488734216No Hit
CTTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1110700.12350954135392901No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT1091400.12136338654333133No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCTC1036850.11529744121078714No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATCT1010180.11233174438184208No Hit
CCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATATCTCGTATG996820.11084611597409158No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC994110.11054476470275895No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATATC964550.10725770065087982No Hit
TTGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAAT941510.10469565884589692No Hit
CTTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA938660.10437873961220762No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT938430.10435316367405024No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATATCTC934180.10388056481679428No Hit
TTAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT926840.10306435879038045No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT924740.10283083935503044No Hit
TGGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAA912480.10146753065151089No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)