FastQCFastQC Report
Sat 1 Jul 2023
SRR2033092.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2033092.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50140701
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA10672972.128604065587356No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG7689981.5336801932625554No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG6034251.203463429839164No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT5303541.0577315223415005No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA5238991.0448577493960447No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT4679360.9332458275762838No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATATCTC4571260.91168649596662No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATATCTCGT4522920.902045625568737No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3697260.7373770063565724No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATATCTCGT3499510.6979379885414845No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2828450.5641026039903191No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT2704560.5393941341187073No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATATC2393570.4773706693889262No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATCT2315360.46177256277290574No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC2214530.4416631510596551No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCTC2161640.43111483423416835No Hit
CCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATATCTCGTATG2160050.43079772658144533No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATATCTC2139220.4266434168919976No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATATCTCG2034920.4058419526284644No Hit
TCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATATCTCGTATG1654720.3300153302603408No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCTGTAGGCACCATCAATATCTCGTA1600850.31927156343506247No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCTGTAGGCACCATCAATATCTC1421920.2835859833710741No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1357620.2707620701194425Illumina PCR Primer Index 6 (96% over 29bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCGT1341080.26746335277602123No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTAGGCACCATCAATAT1334710.2661929277773759No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCTGTAGGCACCATCAATATCTCGTA1253950.25008625228434683No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA1210820.241484457905764No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA1170190.2333812604654251No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATC1102410.21986330027575798No Hit
TGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT1086660.2167221395648218No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAT1063460.21209515997791892No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCCTGTAGGCACCATCAATATCTCG1040140.20744424773798037No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1033180.20605615386190948No Hit
TTCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATATCT975070.19446676662936963No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATCT877710.17504940746640138No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT856400.17079936716481087No Hit
CCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATATCTCGTATGC854020.17032470287960275No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAATA839380.1674049192092468No Hit
CAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA814920.16252664676546905No Hit
TTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATA811780.16190040901103475No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATA782480.15605685289481694No Hit
TCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATATCTCGTATGC749320.14944346310595058No Hit
AAATGGATTTTTGGAAATAGGAGATGGACTGTAGGCACCATCAATATCTC721140.143823278418066No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATA720870.14376942994873565No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCTGTAGGCACCATCAATATCTCGTAT715680.14273434270494145No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATA707730.14114880444132602No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAATATCTCGTAT701360.13987837944268072No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA690300.13767258658788994No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATATC682980.13621269475271197No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATATCTCGTA679300.13547876005961704No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT677350.13508985444778682No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAT669400.1335043161841714No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATATCT622310.12411274425541038No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCTGTAGGCACCATCAATATCTCGTAT589000.11746943865024943No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAA584640.11659988558995217No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTGTAGGCACCATCAATATC577800.11523572436691701No Hit
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGCTGTAGGCACCATCAATAT569250.11353052283812307No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATATCTCGTATG567900.1132612804914714No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA561670.11201877692136773No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAATAT556990.11108540345297527No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT538700.10743766825278331No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT531070.1059159503972631No Hit
TGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGTAGGCACCATCAATA521770.104061169786996No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATATC521200.10394748968507642No Hit
TTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCTGTAGGCACCATCAA520490.10380588815461515No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAT512520.10221636111549379No Hit
GCATTGGTGGTTCAGTGGTAGAATTCCTGTAGGCACCATCAATATCTCGT503430.10040346264803916No Hit
GCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG502260.10017011928094104No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)