FastQCFastQC Report
Fri 30 Jun 2023
SRR2043966.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2043966.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19101972
Total Bases914.2 Mbp
Sequences flagged as poor quality0
Sequence length35-51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACCATCAAT4768862.4965275836442435No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3850742.0158861085127757No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATCAAT3505041.8349100291844211No Hit
AAGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACCATCAAT2057741.077239564585269No Hit
AGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACTATCAAT1685220.8822230500599625No Hit
GTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACCATCAAT1500450.7854948169749175No Hit
AGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGTACCATCAAT1492830.7815057000397656No Hit
AGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCATCATCAAT1390250.727804438201459No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGTACCATCAAT1314080.6879289740347228No Hit
AGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGACCATCAAT1233050.6455092699329682No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACTATCAAT1191990.6240141070251805No Hit
AGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACATCAAT1169230.6120991068356711No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGACCATCAAT1100200.5759614766475419No Hit
TGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACCATCAAT1093550.5724801606870746No Hit
AGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACCATTAAT1064130.5570786094755034No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCACCAT1013580.530615373114357No Hit
AGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGCACCATCAAT959510.5023093950718805No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCATCATCAAT902520.4724747790437553No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACATCAAT845570.44266110326200875No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGCACCATCAAT803280.42052202777807446No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCACCATC793090.41518750001308763No Hit
ATCCTGGGGCTGGAGTCGGTCCCAAGGGTTGCTGTAGGCACCATCAAT718060.3759088328681458No Hit
TTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATCAAT710910.37216576382794403No Hit
AAGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACTATCAAT704620.3688729100848855No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATTAAT698270.3655486459722588No Hit
GTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCACCAT687520.3599209547579695No Hit
TAGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACCATCAAT659860.3454407743870633No Hit
TATCCTGGGGCTGGAGTCGGTCCCAAGGGTTGCTGTAGGCACCATCAAT650120.3403418243938374No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCACCATCA645090.33770858841170953No Hit
AAGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGTACCATCAAT624690.3270290627585466No Hit
GTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACTATCAAT596480.3122609539999326No Hit
AGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCCCATCAAT595900.3119573204274407No Hit
CCAAGTAGCACGGGGCCCGAGAAATCCCGTGTGAATCTGTAGGCACCATC580160.3037173334774022No Hit
AAGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCATCATCAAT577530.30234051227799935No Hit
TGTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCACCA568490.2976080165964017No Hit
GTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGTACCATCAAT524170.2744062236087458No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCCCATCAAT518910.27165258120994No Hit
AAGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGACCATCAAT510190.2670876074993723No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCCTGTAGGCACCATCAAT498330.2608788244480727No Hit
GTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCATCATCAAT490930.2570048788680038No Hit
AAGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACATCAAT483800.25327227995099144No Hit
AGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCAACATCAAT461070.24137298494626627No Hit
AAGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACCATTAAT448260.23466687104347134No Hit
GTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACATCAAT420430.22009769462545542No Hit
GTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGACCATCAAT420420.22009245956386073No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATCAA418030.21884127984273036No Hit
TGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACTATCAAT410680.2149935095706349No Hit
AAGGGCATGGCTGGTTGGCTACGTTCGGGACTGTAGGCACCATCAAT409740.21450141378073426No Hit
GTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACCATTAAT387920.20307850938112568No Hit
CAAGGGCATGGCTGGTTGGCTACGTTCGGGACTGTAGGCACCATCAAT374170.19588029968843007No Hit
AAGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGCACCATCAAT373570.19556619599274883No Hit
TGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGTACCATCAAT367840.19256650569899275No Hit
GTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGCACCATCAAT354550.1856091088396528No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCAACATCAAT350390.1834313232162627No Hit
TGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGACCATCAAT347790.1820702072016439No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTACTACTGTAGGCACCATC340850.17843707445493062No Hit
AGCGGGAAGCCTGCTTCGAGATGAGGACTCCCCTGTAGGCACCATCAAT334000.17485105726256953No Hit
AAGTAGCACGGGGCCCGAGAAATCCCGTGTGAATCTGTAGGCACCATCAA331050.17330671409213666No Hit
TGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCATCATCAAT326310.1708252948962547No Hit
TCAAGTAGCACGGGGCCCGAGAAATCCCGTGTGAATCTGTAGGCACCATC323240.16921813098668556No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATCA303840.1590621114929914No Hit
ATCCTGGGGCTGGAGTCGGTCCCAAGGGTTGCTGTAGGCACTATCAAT294010.1539160459454134No Hit
TGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACATCAAT288310.1509320608364414No Hit
ATTCCGGGAGTAGTGGCGAGCGAAACCGGATGCTGTAGGCACCATCAAT288290.150921590713252No Hit
ATCCTGGGGCTGGAGTCGGTCCCAAGGGTTGCTGTAGGTACCATCAAT285310.14936154235803506No Hit
TTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGTACCATCAAT267310.1399384314875972No Hit
TGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGCACCATCAAT266600.13956674211437436No Hit
ATCCTGGGGCTGGAGTCGGTCCCAAGGGTTGCTGTAGGACCATCAAT266420.13947251100566999No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCACTAT265440.1389594749693906No Hit
ATCCTGGGGCTGGAGTCGGTCCCAAGGGTTGCTGTAGCACCATCAAT265050.13875530756719778No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCACTATC258190.135164055313242No Hit
AAGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCCCATCAAT257680.1348970671719129No Hit
TTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGACCATCAAT256540.13430027015011853No Hit
GGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATCAAT247260.12944213299024834No Hit
TTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACTATCAAT246060.12881392559888583No Hit
TGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACCATTAAT242110.12674607626898415No Hit
AGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACCTCAAT236250.12367833017449717No Hit
AAGTAGCACGGGGCCCGAGAAATCCCGTGTGAACTGTAGGCACCATCAAT234990.1230187124135665No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGTACCAT233740.12236432971423056No Hit
ATCCTGGGGCTGGAGTCGGTCCCAAGGGTTGCTGTAGGCATCATCAAT229890.12034883100027578No Hit
TATCCTGGGGCTGGAGTCGGTCCCAAGGGTTGCTGTAGGACCATCAAT229510.12014989865967765No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGTACCATC226800.11873119696751727No Hit
GTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATCAAT225370.11798258315947693No Hit
TATCCTGGGGCTGGAGTCGGTCCCAAGGGTTGCTGTAGGCACTATCAAT222500.11648012048180156No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGACCATC222340.11639635949628657No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCACTATCA220990.11568962618100372No Hit
CTGTAGGCACCATCAATAGATCCGGAAGAGCACACGTCTGAACTCCAGTC220750.11556398470273122Illumina Multiplexing Index Sequencing Primer (96% over 29bp)
ATCCTGGGGCTGGAGTCGGTCCCAAGGGTTGCTGTAGGCACATCAAT214810.11245435811548671No Hit
GTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCCCATCAAT213570.11180521047774543No Hit
TAGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACTATCAAT213130.11157486776757918No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGACCATCA209900.10988394287249503No Hit
AGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACCACAAT209780.10982112213335879No Hit
TATCCTGGGGCTGGAGTCGGTCCCAAGGGTTGCTGTAGGTACCATCAAT208020.10889975129269376No Hit
AGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAAGCACCATCAAT207630.10869558389050094No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCATCAT205540.10760145601721122No Hit
AGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACCATAAT203310.10643403728159584No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCCTGTAGGTACCATCAAT201470.10547078594817332No Hit
TATCCTGGGGCTGGAGTCGGTCCCAAGGGTTGCTGTAGCACCATCAAT199920.10465935140099672No Hit
AGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTGGCACCATCAAT199390.10438189313647826No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCATCATC198020.10366468969800606No Hit
AAGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCAACATCAAT193720.10141361321229032No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGTACCATCA191390.10019384386072809No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)