FastQCFastQC Report
Fri 30 Jun 2023
SRR2043968_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2043968_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23725610
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6070382.5585769975987973No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATCAAT3558581.4998897815482932No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCACCAT3193811.346144524840457No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATCA1982660.8356623918204843No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCACCA1683970.7097688952992146No Hit
TGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATCAAT1548140.6525185232329116No Hit
GTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCACCAT1481000.6242199884428683No Hit
TTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATCAAT1464730.6173624197649713No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCACCATC1423790.6001068044193595No Hit
TGGAGGTGACCGGTACTAATAGGCCGAGCTGTAGGCACCATCAAT1371390.5780209655304963No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGTACCATCAAT1266740.5339125105740169No Hit
AGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACCATCAAT1175090.49528336679225526No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACTATCAAT1154890.48676936019769357No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGACCATCAAT1061870.4475627813152117No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGCACCATCAAT1048120.44176735603426No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCACCAT974030.41053949719311744No Hit
CAAGGGCATGGCTGGTTGGCTACGTTCGGGACTGTAGGCACCATCAAT972360.4098356164499037No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCACTAT911500.3841840104427241No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCACCATCA866600.36525931261619826No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACATCAAT812580.3424906672578703No Hit
AAGGGCATGGCTGGTTGGCTACGTTCGGGACTGTAGGCACCATCAAT781570.3294204026787931No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGTACCAT770210.32463232768303957No Hit
GTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATCAAT762960.32157655798944684No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGACCATC756900.3190223560110783No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCATCATCAAT755000.31822153360861954No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATTAAT742660.31302040284738725No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCATCAT684630.2885616007343963No Hit
TATGCTGTCGAGAAAAGCCTCTGTAGGCACCATCAAT663040.2794617293296147No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATCAA658500.27754818527321323No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGCACCATC595260.25089344383558526No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCACTATC570330.24038581094437614No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCACATC555110.23397080201520634No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGACCATCAA519480.2189532745417294No Hit
TGTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCACCA513180.21629791604936607No Hit
TGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGACCATCAAT507380.21385330029449187No Hit
TGGAGGTGACCGGTACTAATAGGCCGAGCTGTAGGCACTATCAAT505740.2131620641155275No Hit
TCAAGGGCATGGCTGGTTGGCTACGTTCGGGACTGTAGGCACCATCAAT505710.2131494195512781No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCCCATCAAT499630.21058678786341004No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGTACCATC494250.20831919600802679No Hit
AGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACTATCAAT487550.20549524332567215No Hit
TGGAGGTGACCGGTACTAATAGGCCGAGCTGTAGGACCATCAAT481170.20280616599531054No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGTACCATCA480110.20235939139183354No Hit
TGGAGGTGACCGGTACTAATAGGCCGAGCTGTAGGTACCATCAAT477530.20127195886638954No Hit
TGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGTACCATCAAT473520.19958180211172655No Hit
CTAATACCGGATACTGACCCTCGCAGGCATCTGCGAGCTGTAGGCACCAT470980.19851122900528162No Hit
TTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACTATCAAT457630.19288439791432127No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCACTA456850.1925556392438382No Hit
TTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGTACCATCAAT453590.19118159659540893No Hit
TTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGACCATCAAT449920.18963474490223856No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGACCATCA446180.1880583892258197No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCACCATC444490.18734607877310636No Hit
TGGAGGTGACCGGTACTAATAGGCCGAGCTGTAGCACCATCAAT437350.18433667248176128No Hit
AGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGTACCATCAAT432540.18230932734711564No Hit
GTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCACTAT429670.1810996640339279No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACTATCA429480.18101958179368202No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCATCATC421880.1778162921838469No Hit
TGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACTATCAAT417610.17601654920568954No Hit
TGGAGGTGACCGGTACTAATAGGCCGAGCTGTAGGCATCATCAAT414340.1746382917025105No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGCACCATCA412020.17366044540056083No Hit
AGCGGGAAGCCTGCTTCGAGATGAGGACTCCCCTGTAGGCACCATCAAT410470.17300714291434446No Hit
AGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGACCATCAAT401280.16913369139929385No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCATCATCA397120.16738031182338411No Hit
ATGCTGTCGAGAAAAGCCTCTGTAGGCACCATCAAT388390.16370074362682352No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCACCATT386730.1630010777383595No Hit
AGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGCACCATCAAT386050.16271446761537425No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGACCAT382050.16102852571546106No Hit
TAATACCGGATACTGACCCTCGCAGGCATCTGCGAGCTGTAGGCACCATC378250.1594268809105435No Hit
AGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCATCATCAAT373990.15763135278713591No Hit
GTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGTACCAT369880.1558990474849751No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCAACATCAAT367180.1547610367025337No Hit
TGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGCACCATCAAT365680.15412880849006622No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGCACCATCAA361410.15232906551190886No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGTACCA361140.1522152644336647No Hit
GGAGGTGACCGGTACTAATAGGCCGAGCTGTAGGCACCATCAAT360740.1520466702436734No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCACATCA355450.14981701208103818No Hit
TTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGCACCATCAAT353740.14909627191882527No Hit
TCGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGGCACCATCAAT344880.1453619106105175No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCATCA344100.1450331519400344No Hit
TGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCATCATCAAT341480.14392885999559127No Hit
TAAGGGCATGGCTGGTTGGCTACGTTCGGGACTGTAGGCACCATCAAT341340.1438698520290943No Hit
AGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACATCAAT340580.14354952306811078No Hit
GTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGACCATC339690.1431744009953801No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCACTATCA339500.1430943187551342No Hit
GTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCACCA337730.14234828946442263No Hit
CAAGGGCATGGCTGGTTGGCTACGTTCGGGACTGTAGGACCATCAAT334870.14114284100598468No Hit
AGTAGCACGGGGCCCGAGAAATCCCGTGTGACTGTAGGCACCATTAAT333340.14049796822926788No Hit
TGGAGGTGACCGGTACTAATAGGCCGAGCTGTAGGCACCATTAAT328730.13855492018961788No Hit
TGGAGGTGACCGGTACTAATAGGCCGAGCTGTAGGCACATCAAT328690.13853806077061875No Hit
GTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCATCAT320330.13501444219980013No Hit
CAAGGGCATGGCTGGTTGGCTACGTTCGGGACTGTAGGCACTATCAAT311380.1312421471987443No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACCTGTAGGCACCATCAAT310590.13090917367351143No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGCACCAT309940.13063520811477555No Hit
TGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACATCAAT307890.12977116289107002No Hit
TTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACATCAAT305170.12862472239912906No Hit
TGGAGGTGACCGGTACTAATAGGCCGACTGTAGGCACCATCAAT300190.1265257247337371No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCACAT298130.1256574646552818No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGTACCATCA297600.12543407735354328No Hit
GTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGTACCATCAAT294730.12422441404035556No Hit
CTGTAGGCACCATCAATAGATCCGGAAGAGCACACGTCTGAACTCCAGTC294440.12410218325261185Illumina Multiplexing Index Sequencing Primer (96% over 29bp)
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCAACAT294430.12409796839786207No Hit
TTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCATCATCAAT293280.12361326010163702No Hit
CAAGGGCATGGCTGGTTGGCTACGTTCGGGACTGTAGCACCATCAAT292380.12323392317415655No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGACCATCAA290720.12253425728569255No Hit
GTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGCACCATC288480.12159012982174114No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCACTAT287980.121379387084252No Hit
AAGGGCATGGCTGGTTGGCTACGTTCGGGACTGTAGGACCATCAAT287870.1213330236820044No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCCCATC287470.12116442949201306No Hit
TTGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATC285410.12029616941355775No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACATCAA281880.11880832568688435No Hit
CAAGGGCATGGCTGGTTGGCTACGTTCGGGACTGTAGGTACCATCAAT279770.11791899133468012No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATTA276240.1164311476080067No Hit
TTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATTAAT273270.11517933574732116No Hit
AAGGGCATGGCTGGTTGGCTACGTTCGGGACTGTAGGCACTATCAAT272530.11486743649583721No Hit
GGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATCAAT271750.11453867782535412No Hit
TGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATTAAT264460.11146604871276229No Hit
ACCCCGGGACCTTTACTACAGTTTGATATTGGCTGTAGGCACCATCAAT261760.11032803793032088No Hit
GTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCACATC258220.10883597934889767No Hit
AAGGGCATGGCTGGTTGGCTACGTTCGGGACTGTAGGTACCATCAAT253150.10669904799075766No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCATCATCA253000.10663582516951094No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGTACCAT248770.10485294161035269No Hit
GTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGCACCATCAAT245990.10368121198991301No Hit
AAGGGCATGGCTGGTTGGCTACGTTCGGGACTGTAGCACCATCAAT245530.103487328671423No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGCACCATCAA243270.10253477149797202No Hit
GTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGACCATCAAT243210.10250948236947334No Hit
CAAGGGCATGGCTGGTTGGCTACGTTCGGGACTGTAGGCATCATCAAT240230.10125345565403798No Hit
CAAGGGCATGGCTGGTTGGCTACGTTCGGGAGCTGTAGGCACCATCAAT240170.10122816652553927No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)