FastQCFastQC Report
Sun 2 Jul 2023
SRR2043969_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2043969_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22247790
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGGCACCATCAAT3953041.7768236755201303No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCACCA2534431.1391828132142563No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATCA2403221.0802061687924958No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1760330.791238140956922No Hit
TCGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGGCACTATCAAT1632370.7337223157895683No Hit
TCGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGGTACCATCAAT1429750.6426481012271331No Hit
TCGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGCACCATCAAT1323670.5949669607632938No Hit
TCGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGGACCATCAAT1317030.5919823946558288No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCACCAT1251160.5623749594903584No Hit
CCGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGGCACCATCAAT1189490.5346553522844292No Hit
TCGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGGCATCATCAAT1157360.5202134683939393No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGACCATCAA1111690.4996855867481669No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGTACCATCA1029470.4627291070259113No Hit
TCGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGGCACCATTAAT992250.4459993554415967No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATCAAT977190.4392301437580991No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGCACCATCAA917580.41243647121804006No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACTATCA870320.39119391184472707No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGACCAT861200.38709462827543767No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCACTA810990.36452609450197077No Hit
TCGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGGCACATCAAT807090.36277311139668256No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCACCATC778140.34976058296127394No Hit
GTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCACCA729690.3279831389994242No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGTACCA719040.3231961466734449No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGCACCAT713400.3206610634134896No Hit
CGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGGCACCATCAAT710110.3191822648451824No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCATCATCA694730.3122692186504817No Hit
TGGAGGTGACCGGTACTAATAGGCCGAGCTGTAGGCACCATCAAT664030.2984700952319309No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATCAA653800.2938718857019057No Hit
ACCCCGGGACCTTTACTACAGTTTGATATTGGCTGTAGGCACCATCAAT646340.2905187436594826No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCATCA591730.26597248535697254No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATTA583960.2624800036318214No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCACAT566390.2545825900010743No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACATCAA559950.25168792046311117No Hit
TTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATCAAT507010.2278922985159425No Hit
CCGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGGCACTATCAAT499920.22470546512709805No Hit
TCGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGGCAACATCAAT497560.2236446856069749No Hit
AAGGGCATGGCTGGTTGGCTACGTTCGGGACTGTAGGCACCATCAAT496800.22330307864286744No Hit
CAAGGGCATGGCTGGTTGGCTACGTTCGGGACTGTAGGCACCATCAAT488930.21976564863296533No Hit
TTGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATC483070.21713167914655793No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGTACCATCAAT481030.21621473413763795No Hit
TGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATCAAT467970.21034448814915996No Hit
AGCGGGAAGCCTGCTTCGAGATGAGGACTCCCCTGTAGGCACCATCAAT455370.20468100427053654No Hit
CCGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGGTACCATCAAT454230.20416859382437536No Hit
TCGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGGCCCATCAAT439950.19774997876193545No Hit
CCGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGCACCATCAAT419290.18846366313238302No Hit
CCGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGGACCATCAAT415930.18695340076475012No Hit
GCGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGGCACCATCAAT413810.18600049712802935No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCACTAT405170.1821169653255447No Hit
TAAGGGCATGGCTGGTTGGCTACGTTCGGGACTGTAGGCACCATCAAT401660.1805392805307853No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGACCATC396320.178139042125083No Hit
TGGAGGTGACCGGTACTAATAGGCCGAGCTGTAGCACCATCAAT395940.17796823864302927No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATCTGTAGGCACCAT394740.1774288592260175No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGCACCATCAAT386800.17385996541678972No Hit
TGGAGGTGACCGGTACTAATAGGCCGAGCTGTAGGACCATCAAT384990.17304640146279698No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACTATCAAT384300.1727362582980152No Hit
TGGAGGTGACCGGTACTAATAGGCCGAGCTGTAGGTACCATCAAT378720.17022814400891054No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCCCATCAA374520.16834031604936942No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGTACCAT373330.16780543146083274No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGCACCATC372930.1676256383218288No Hit
TGGAGGTGACCGGTACTAATAGGCCGAGCTGTAGGCACTATCAAT372530.1674458451828249No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCACTATC370210.16640304497660216No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACTGTAGGCACCATC369470.16607042766944494No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGACCATCA366900.16491525675134475No Hit
TCAAGGGCATGGCTGGTTGGCTACGTTCGGGACTGTAGGCACCATCAAT362490.16293303739382653No Hit
CCGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGGCATCATCAAT353180.15874835208351032No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGTACCATC349750.1572066259165517No Hit
CGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGGCACTATCAAT345880.1554671272966888No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGACCATCAAT344240.15472997542677272No Hit
CGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGCACCATCAAT330520.14856307075893832No Hit
CGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGGTACCATCAAT318070.14296700930744133No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGCACCATCA317480.14270181442741053No Hit
CTGGAAACGGGGTCTAATACCGGATACTGACCCTCTGTAGGCACCATCAA310220.13943856895448942No Hit
TCGGCGTAGAGGGTAAGACCCCCGTAGTCGCTGTAGGCACCATCAAT308110.13849016014624374No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGTACCATCAA305900.1374968030532471No Hit
CCGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGGCACCATTAAT305830.1374653392539214No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCATCAT303130.13625173556564496No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACTGTAGGCACCATCA294220.13224684339433265No Hit
TGGAGGTGACCGGTACTAATAGGCCGAGCTGTAGGCATCATCAAT293700.13201311231362756No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCACATC292100.1312939397576119No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGCACCATCAAT279820.12577429039019156No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACCATTAAT278670.12525738511555531No Hit
CTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCACCA277730.12483487123889608No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCATCATC277660.12480340743957041No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCCCAT273960.12314032090378416No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCAACATCA268490.12068164972790554No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGACCATCAAT258650.11625873850840915No Hit
CGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGGACCATCAAT256130.11512604173268447No Hit
TTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCAACA256090.11510806241878406No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCACATCA253780.11406975704103643No Hit
CGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGGCACCATTAAT251970.1132561930870437No Hit
CGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGGCATCATCAAT251310.11295953440768725No Hit
CCGGCGTAGAGGGTAAGACCCCCGTAGTCCTGTAGGCACATCAAT250800.11273029815545724No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCACCATT243280.10935018714218357No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACCTGTAGGCACCATC243190.10930973368590768No Hit
GTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGACCAT241770.10867146804244376No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCATCATCAAT240220.10797476962880358No Hit
TGTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCACC238580.10723761775888752No Hit
TGGAGGTGACCGGTACTAATAGGCCGAGCTGTAGGCACCATTAAT236530.10631617792149244No Hit
TTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGTACCATCAAT235540.10587118990245774No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACTATCAA232650.10457218447315442No Hit
GTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTCTGTAGGCACTA231190.1039159395157901No Hit
ACCCCGGGACCTTTACTACAGTTTGATATTGGCTGTAGCACCATCAAT228850.10286414965261718No Hit
TGTGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGTACCATCAAT225270.10125500105853211No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTCTGTAGGCACATCAAT224760.10102576480630211No Hit

[WARN]Adapter Content

Adapter graph