FastQCFastQC Report
Fri 30 Jun 2023
SRR2052911.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052911.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16250647
Total Bases828.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA5493713.380610015096629No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC2395611.474162844100915No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1553340.9558634803894269No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1489910.9168311883213018No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1007330.6198707042248841No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT913670.5622360758928552No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC839630.5166748130089837No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG788620.48528529356400396TruSeq Adapter, Index 2 (100% over 49bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA759590.46742138943760214No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG716090.4406532244531556No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC668460.4113436221954732No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC657810.4047900369751432No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC652590.4015778571770096No Hit
TCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGAGATCGGAAGAG623500.38367703144373266No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG562910.34639236210102897No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA560260.3447616577973788No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG541850.33343287808786937No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG532540.3277038754210832No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT516970.31812271843699513No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTC480530.2956989958615186TruSeq Adapter, Index 2 (100% over 50bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC444250.27337373090437567No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGAGATCGGAAGAGCACA431890.2657678798881054No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC401720.24720246523107664No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC388730.2392089373426178No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA385000.23691364411521587No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACAC368420.22671097341539692No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCA360630.22191731812277998No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA355220.21858821990287525No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG349380.214994516833699No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT269700.16596262290356809No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC267110.164368840206793No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAGATCGGAAGAGCACAC265460.16335349601772778No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA247430.15225855315176068No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC236030.14524344784549195No Hit
TTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCAGATCGGAAGAGC234630.14438194368507296No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC228000.14030210612537458No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC226540.1394036803580805No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACG218260.13430849860931693No Hit
ATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCAGATCGGAAGAGCAC212030.1304748050954525No Hit
AGACGATCAGATACCGTCGTAGTTCCGACCATAAGATCGGAAGAGCACAC209750.12907178403419878No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC202000.12430274314616521No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACAGATCGGAAGAGCAC198580.12219821155428458No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG197940.12180438108095018No Hit
ACGGTCTGGGGAAAGGCTCCTGTGTTGTTGAGCCTGCCAAGATCGGAAGA189720.11674612093906168No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGC187230.1152138742537451No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGATCGGAAGAG182190.11211245927623681No Hit
TTGACGCGATGTGATTTCTGCCCAGTGCTCTGAGATCGGAAGAGCACACG174800.10756494802945385No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACAC170510.10492505313788429No Hit
AAGACGATCAGATACCGTCGTAGTTCCGACCATAAGATCGGAAGAGCACA167320.10296205437235822No Hit
TGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGAGATCGGAAGAGCAC164280.1010913596240199No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)