FastQCFastQC Report
Sat 1 Jul 2023
SRR2052912.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052912.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18073831
Total Bases921.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA4078372.256505552143317No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC3143861.73945413122431No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC2009501.1118284773161817No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1087520.6017097316003452No Hit
AGTGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGT997970.5521629586997909No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG839300.46437304852524075No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC822850.4552714916942623No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA808360.4472543756771877No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC702240.38853965161010967No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA690040.38178956082968796No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC664050.36740965432287154No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACAGATCGGAAGAGCACACGT654070.36188785875003476No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC640920.35461214614654746No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAGATCGGAAGAGCACACG589960.3264166849850483No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA567040.3137353668959281No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA566680.3135361838892928No Hit
TGTAGTATCTGTTCTTATCAGTTTAATATCTGATAGATCGGAAGAGCACA510080.2822201889571724No Hit
ACTCCGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGAAGAGCACACG501950.27772197272398974No Hit
TGGTAAGTGTACTGGAAAGTGCACTTGGACGAACAGATCGGAAGAGCACA484700.2681777869893771No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT484040.26781261814387886No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG481240.26626341698115913TruSeq Adapter, Index 7 (100% over 49bp)
AATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAGCA440110.24350675847306527No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGC428590.23713290226073266No Hit
TCGCTGCGATCTATTGAAAGTCAGATCGGAAGAGCACACGTCTGAACTCC423080.23408429568695205Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA408650.22610037683764997No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG400420.22154683199151304No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCAGATCGGA390970.21631827806733395No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC385750.2134301244711207Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCAGATCGGAAGAGCA316960.17536957161987404No Hit
TAACTGTTAGTCCAAAGAGGAGATCGGAAGAGCACACGTCTGAACTCCAG307690.17024060919901265Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAAGATCGGAAGAGCACA297490.16459709067767647No Hit
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA266570.14748948355221425Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCAGATCGGAAGAGC263030.14553085065363286No Hit
TTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCAAGATCGGAAGAG248410.13744180743971768No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT238430.1319200118668809Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG237600.13146078437936043No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCA232710.1287552152058963No Hit
ATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAAGATCGGAAGAGCACAC206190.11408206705042223No Hit
TGGCTAAGATCAAGTGTAGTATCTGTTCTTAAGATCGGAAGAGCACACGT198940.1100707426112372No Hit
ATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGAGATCGGAAGAGCACA198660.10991582249496523No Hit
TCGCTGCGATCTATTGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAG197600.1093293391976499Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAA188820.10447148698026446No Hit
CAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCAGATCGGAAGAGC188440.1042612382510382No Hit
AAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAAGATCGGAAGAGCAC184370.10200936370379915No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)