FastQCFastQC Report
Sat 1 Jul 2023
SRR2052913.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052913.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18948536
Total Bases966.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA4369052.305745414843659No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG3759111.983852472824286No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACAC3408791.7989727544122671No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG3156671.6659176202319799No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG2769681.4616854832478878No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC2627831.3868248185506258No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC2184141.1526695254979065No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1984201.0471521388248675No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA1712390.9037057005353869No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT1603910.8464558950622887No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGAGATCGGAAGAGCACA1406070.7420467734288285No Hit
TGGCTAAGATCAAGTGTAGTATCTGTTCTTAAGATCGGAAGAGCACACGT1320810.6970512128219298No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG1284280.6777726785858285No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1113080.5874226905973106No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1065910.5625289468273432No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC1027450.5422318642453433No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC920400.4857367344896725No Hit
GGCTAAGATCAAGTGTAGTATCTGTTCTTAAGATCGGAAGAGCACACGTC909620.48004764061983474No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC881450.4651810567317708No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG829140.43757470234112017TruSeq Adapter, Index 3 (100% over 49bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA805590.42514630154012956No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT798540.42142569747868647No Hit
TCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGAGATCGGAAGAG778540.4108707923398409No Hit
TAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGAACTCCA751450.3965741733292746Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCAGATCGGAAGAGCAC722350.3812167863522543No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACA706810.37301562505937136No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAGATCGGAAGAGCACAC704320.37170153936958505No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACG630550.33276977176495326No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG619390.3268801346974774No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC617360.3258088118258846No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC611080.3224945716122871No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCA568520.30003373347682377No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA564760.29804941131072077No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTC550600.2905765384724181TruSeq Adapter, Index 3 (100% over 50bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC537730.28378445701557103No Hit
ACGGTCTGGGGAAAGGCTCCTGTGTTGTTGAGCCTGCCAAGATCGGAAGA515970.272300720224507No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG513450.2709708021770125No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC499410.26356125876954295No Hit
AGTGTAGTATCTGTTCTTATCAGTTTAATATCTGAGATCGGAAGAGCACA491140.25919680549463026No Hit
AAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGAGATCGGAAGAGCAC441960.23324229375820907No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC438480.23140574026404995No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT388160.20484959893471455No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA385500.20344579655124806No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC367930.19417331238677224No Hit
TGTAGTATCTGTTCTTATCAGTTTAATATCTGAGATCGGAAGAGCACACG365020.19263757368907022No Hit
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA358820.18936555309602812TruSeq Adapter, Index 3 (100% over 36bp)
TATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACAC346810.18302733256015136No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACG324650.17133249766631048No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG317080.16733746607125743No Hit
TTGGCTAAGATCAAGTGTAGTATCTGTTCTTAAGATCGGAAGAGCACACG314390.1659178313300827No Hit
TTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTAGATCGGAAGAGCAC307850.16246637734968022No Hit
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC295740.15607538228810922Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG282630.14915664196959597No Hit
ATTCGGCTGATCTGGCTGGCTAGGCGGGTGAGATCGGAAGAGCACACGTC277500.14644930880148208No Hit
TAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAACTCCAG275750.1455257546018331Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGCTAAGATCAAGTGTAGTATCTGTTCTTATCAGATCGGAAGAGCACAC267300.14106630718067084No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC266620.1407074404059501No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGC263350.13898171341574886No Hit
ATGGTTAGCACTCTGGACTCAGATCGGAAGAGCACACGTCTGAACTCCAG250300.1320946378126521Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGAGATCGGAAGAGCAC245110.1293556399291217No Hit
TTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCAAGATCGGAAGAG220880.1165683723534103No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT210670.11118009328002965No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCAGATCGGAAGAGCACA210210.11093733046183621No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGT208620.11009821550329799No Hit
ACTCCGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGAAGAGCACACG208190.10987128504281281No Hit
TCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTAGATCGGAAGAGCACA206500.10897939555858036No Hit
TTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCAGATCGGAAGAGC204950.10816139041031983No Hit
TGTAGTATCTGTTCTTATCAGTTTAATATCTGATAGATCGGAAGAGCACA202160.10668898114345086No Hit
GTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT202030.10662037426004836Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGAGATCGGAAGAGCACAC201700.10644621832525743No Hit
ATTCGGCTGATCTGGCTGGCTAGGCGGGGGAGATCGGAAGAGCACACGTC196760.10383915675596256No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTCAGATCGGAAGAGCACA196610.10375999496742122No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC193860.10230869551082997No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)