FastQCFastQC Report
Fri 30 Jun 2023
SRR2052914.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052914.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26865068
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC7503682.793099202280076No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC5849352.1773069772241036No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA2694881.003116761141271No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC2414000.8985646341933696No Hit
AGTGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGT1874380.697701565467841No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG1833160.6823582207199327No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1800970.6703761181620683No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC1653450.6154646621404419No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA1593460.5931345492965066No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACAGATCGGAAGAGCACACGT1477230.5498701883054977No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1447680.5388707744942243No Hit
AATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAGCA1306900.48646815262109144No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG861480.32066920508073904No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC781030.2907232544507239No Hit
TAACTGTTAGTCCAAAGAGGAGATCGGAAGAGCACACGTCTGAACTCCAG777150.28927899977770394Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCAGATCGGA754540.28086286623208995No Hit
TGTAGTATCTGTTCTTATCAGTTTAATATCTGATAGATCGGAAGAGCACA748910.2787672080338676No Hit
AATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAAGATCGGAAGAGCACA746590.27790363307474225No Hit
ATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGAGATCGGAAGAGCACA736290.27406965804069433No Hit
ACTCCGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGAAGAGCACACG721770.2686648699344442No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG703530.2618753840489069TruSeq Adapter, Index 8 (100% over 49bp)
TCGCTGCGATCTATTGAAAGTCAGATCGGAAGAGCACACGTCTGAACTCC700960.2609187514433241Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC655350.24394131442362255Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA649070.24160370634461079No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT648040.24122030884120596No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGC617850.2299826674550014No Hit
TGGTAAGTGTACTGGAAAGTGCACTTGGACGAACAGATCGGAAGAGCACA616070.22932009701222422No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC602480.22426148335079593No Hit
ATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAAGATCGGAAGAGCACAC553910.20618224379703784No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA508040.1891080268250205No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT492320.18325656201577453Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA464070.17274104796607997No Hit
ATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAGCAC441800.16445147281964817No Hit
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA435350.16205058554104534Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC429420.15984325816707406No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG423170.15751681700563722No Hit
AAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAAGATCGGAAGAGCAC423000.15745353780604612No Hit
TCGCTGCGATCTATTGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAG388950.14477908635853817Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCAAGATCGGAAGAG342690.12755969945804715No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCCTGAGATCGGAAGAGC341440.1270944112257598No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGAGATCGGAAGAGCA324590.12082232585452603No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAGATCGGAAGAGCACACG318610.11859638695126326No Hit
AATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGAGATCGGAAGAGCAC306680.11415567606231258No Hit
AGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATAGATCGGAAGAGCA299980.11166173113725229No Hit
ATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCAGATCGGAAGAGCA295300.10991969199556836No Hit
TGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAGCACACGTCTG278540.10368110737705931Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC277010.10311159458073957No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC273380.10176039755417704No Hit
AAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAGC272060.10126905318088159No Hit
AAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGAGATCGGAAGAGCA269430.10029008674014896No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)