FastQCFastQC Report
Fri 30 Jun 2023
SRR2052915.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052915.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26423896
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA13614955.152514224246114No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC6123882.31755377783806No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA5917642.2395032133035944No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC3218131.2178862647658015No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG2253540.8528416854198941TruSeq Adapter, Index 4 (100% over 49bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1792850.6784957070675726No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1765380.6680998138957254No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA1289440.4879825442849154No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG1182260.4474207739842755No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1109150.4197526360230906No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG1096370.4149161047258133No Hit
ATTCGGCTGATCTGGCTGGCTAGGCGGGTGAGATCGGAAGAGCACACGTC1091720.413156333948635No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1036590.39229264299253974No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT999080.3780971587233011No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACAC975570.3691999090520187No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC951520.3600982989033865No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC943680.357131287528531No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTC826440.3127623572239309TruSeq Adapter, Index 4 (100% over 50bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC788610.2984457704495961No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGAGATCGGAAGAGCACA774930.2932686383567359No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC743990.2815595398952524No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC652000.24674635413339505No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA631150.23885576903572436No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACAC598290.22642005554366396No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCA561460.21248191409775458No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG550380.20828873985880053No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC547980.20738047107057944No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACG517640.1958984398061512No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC501080.18963138516742573No Hit
ATTCGGCTGATCTGGCTGGCTAGGCGGGGGAGATCGGAAGAGCACACGTC436710.16527085937667935No Hit
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA434800.16454802879938674TruSeq Adapter, Index 4 (100% over 36bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC382240.14465694233734497No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT377750.14295772281271468No Hit
TATATTAAATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACA373970.14152719947126646No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC366510.1387039973212126No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGATCGGAAGAG363530.13757623024250473No Hit
TTTTGATCCTTCGATGTCGGCTCTTCCTATCAGATCGGAAGAGCACACGT350510.13264887206640535No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGATCGGAAGAGCACACG349220.13216067759273653No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC343800.13010950391267054No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAAGATCGGAAGAGCACACGT341580.12926935528356606No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA327830.12406573201771609No Hit
TATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACAC327540.1239559828724727No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG318980.12071649086115083No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACAGATCGGAAGAGCAC311170.11776083284614804No Hit
TGGCTAAGATCAAGTGTAGTATCTGTTCTTAAGATCGGAAGAGCACACGT301670.11416560222610626No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGC273340.10344424607181318No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT267000.10104490268959582No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)