FastQCFastQC Report
Fri 30 Jun 2023
SRR2052916.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052916.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13129805
Total Bases669.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG9158556.975389200372739TruSeq Adapter, Index 9 (100% over 49bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA2804652.136094176570025No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC2384901.8164016906572489No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1525191.161624258699958No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC938410.714717392984892No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA917730.6989669686640433No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTC850270.6475876831377161TruSeq Adapter, Index 9 (100% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC849800.6472297189486059No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA797900.6077013329596289No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC789250.6011132686281327Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG768220.5850962752302872No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC694230.5287435723531309No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG569960.43409631750052646No Hit
TGTAGTATCTGTTCTTATCAGTTTAATATCTGATAGATCGGAAGAGCACA531050.4044614523978079No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA527870.402039481926807No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACAGATCGGAAGAGCACACGT493230.37565675956345124No Hit
AGTGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGT484680.3691448578253828No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG483110.3679491051085679Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCAGATCGGA442440.3369737783615217No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA435100.3313834440039285No Hit
ACTCCGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGAAGAGCACACG397600.30282247146854047No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAGATCGGAA360550.27460423060357714No Hit
AGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATAGATCGGAAGAGCA348020.26506105764708615No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC341160.2598363037379458No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCAGATCGGAAGAGCACA332880.25353004100213217No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT325210.2476883700862275No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT310850.23675142167000957Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT302160.23013289230114234Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGTAAGTGTACTGGAAAGTGCACTTGGACGAACAGATCGGAAGAGCACA300480.22885336073155696No Hit
AAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGAAGATCGGAAGAGCA297050.2262409837769868No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCAGATCGGAAGAGCACA289820.220734428272164No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT287710.21912739755083946No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC278300.2119604975092928No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC271530.20680428993423738No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC263100.2003837833082822No Hit
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC248740.18944683489206426Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGCTGCGATCTATTGAAAGTCAGATCGGAAGAGCACACGTCTGAACTCC239940.18274452667042657Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTC234250.17841087510439035Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC234240.1784032588450476No Hit
TTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCAGATCGGAAGAGC225640.17185327581026527No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA222890.16975880449100347No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG216030.16453405058186316No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG211920.16140376799198466Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC198600.15125891054741483Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC196010.14928629937763738No Hit
AAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATAGATCGGAAGAGC189700.1444804397323494No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG188780.14377974387281456No Hit
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA185530.14130445958641427Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACA185240.14108358806547394No Hit
CTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAAC185220.1410683555467884Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGT181760.13843312981418993No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA176120.13413755954486759Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA165420.12598816204810354No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCAGATCGGAAGAGCACA165270.12587391815796198No Hit
TAACTGTTAGTCCAAAGAGGAGATCGGAAGAGCACACGTCTGAACTCCAG155580.11849376285481773Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCTGCGATCTATTGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAG153970.11726754510063174Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACGTCTGAACTC152460.11611748993987343Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC150700.1147770282955459No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA150640.11473133073948928No Hit
AGTGTAGTATCTGTTCTTATCAGTTTAATATCTGAAGATCGGAAGAGCAC147450.11230174400914561No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT142860.1088058809708141No Hit
TCGCTGCGATCTATTGAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT140100.10670379339220955Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGTAAGTGTACTGGAAAGTGCACTTGGACGAGATCGGAAGAGCACACGT137330.10459408955426222No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG136450.10392385873209846No Hit
TCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTCTGAACT135960.10355066202430271Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTC134570.10249200197565767No Hit
AGGGCTCCATAGCTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACG134200.10221020037997518No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG134170.10218735160194686No Hit
TATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGAACTC133670.10180653863480836Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACACGTCTGAACTCCA131570.10020712417282664Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)