FastQCFastQC Report
Sat 1 Jul 2023
SRR2052917.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052917.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31892235
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC12445993.902514201340859No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA11982723.7572531370096827No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA7270462.2796959824233074No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC5398661.69278195773987No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG2622410.8222722552997618No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACAC2253910.7067268882221645No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG1993770.6251584437403023No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1857150.5823204300357124No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG1380650.4329110205038938TruSeq Adapter, Index 5 (100% over 49bp)
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC1355560.4250439017522604No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1301990.40824670958306936No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC1281720.4018909305039299No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGATCGGAAGAG1233770.38685592276615294No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACG1040520.32626123568950244No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC987160.309529890269528No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA958070.3004085477232938No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAGATCGGAAGAGCACAC932300.2923282109265782No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA856870.2686766857198939No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGAGATCGGAAGAGCACA829010.25994101699049943No Hit
TGGCTAAGATCAAGTGTAGTATCTGTTCTTAAGATCGGAAGAGCACACGT818280.2565765616614828No Hit
TCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGAGATCGGAAGAG769670.24133460699759676No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG749480.2350039123943493No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT736470.2309245495024102No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACAGATCGGAAGAGCAC666650.2090320731676535No Hit
TATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACAC655680.20559236441096085No Hit
TAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGAACTCCA636820.19967869921941814Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCA625090.19600068794175135No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC611390.19170497144524365No Hit
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCA591710.1855341903758078No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTC577470.18106915366702897TruSeq Adapter, Index 5 (100% over 50bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG567230.17785834075285098No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC564770.17708699311917148No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACAGATCGGAAGAGCAC559670.1754878577810555No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC559380.17539692655594694No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG509450.1597410780398426No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC506810.15891329033540608No Hit
TCGCTGCGATCTATTGAAAGTCAGATCGGAAGAGCACACGTCTGAACTCC484600.15194921271588524Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA484450.1519021793235877No Hit
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA475820.1491961914867365TruSeq Adapter, Index 5 (100% over 36bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC467160.1464807969714258No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT461210.14461513907695714No Hit
TTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCAGATCGGAAGAG456920.14326998405724778No Hit
ATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCAGATCGGAAGAGCAC430320.13492939582315258No Hit
TCGCTGCGATCTATTGAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT427380.134007541334121Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGTCTGGGGAAAGGCTCCTGTGTTGTTGAGCCTGCCAAGATCGGAAGA426650.13377864549160634No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC411420.12900318839366387No Hit
TGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGAGATCGGAAGAGC408340.12803743607182125No Hit
AGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAGATCGGAAGAG390490.12244046238841523No Hit
TGTAGTATCTGTTCTTATCAGTTTAATATCTGATAGATCGGAAGAGCACA390270.12237148007971219No Hit
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA378410.11865270652872086Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG375570.1177622076345543No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT373520.11711941793982139No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACA372610.11683408202654971No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA365730.11467681709983638No Hit
TCGCTGCGATCTATTGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAG340620.10680342722922995Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAGATCGGAAGAGCA335540.10521056301008692No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT333710.10463675562405708No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG331790.10403472820264871No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)