FastQCFastQC Report
Fri 30 Jun 2023
SRR2052918.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052918.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34039497
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA18440575.417403788310973No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC11059333.2489698658003086No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC3999651.175002674099444Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC3860471.1341148783720276No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC3583961.0528827732090165No Hit
AGTGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGT3306110.9712570077049023No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA2626720.7716682770018605No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG2224730.653573112434652No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC2060920.6054496046166604No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1569440.46106439234398794No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT1536710.4514490916243562No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1476010.43361686572513103No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT1473570.43290005137267457Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1465200.4304411431226496No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACAGATCGGAAGAGCACACGT1458620.42850809458200867No Hit
TCGCTGCGATCTATTGAAAGTCAGATCGGAAGAGCACACGTCTGAACTCC1342710.3944564750765853Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC1331110.39104866913867736No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA1108070.32552478669117824No Hit
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA941020.2764494434215641Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG891450.2618869485644867No Hit
AATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAGCA891190.2618105667072578No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG847950.24910767629733188TruSeq Adapter, Index 10 (100% over 49bp)
TGTAGTATCTGTTCTTATCAGTTTAATATCTGATAGATCGGAAGAGCACA823410.24189840408041283No Hit
AGCAGATCTCTGATGAATAACCAACGAAAAAAGGCGAGATCGGAAGAGCA795960.23383424261527716No Hit
TCGCTGCGATCTATTGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAG788450.23162798204685572Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC766980.22532060329798648No Hit
TAACTGTTAGTCCAAAGAGGAGATCGGAAGAGCACACGTCTGAACTCCAG757730.2226031718388788Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGTAAGTGTACTGGAAAGTGCACTTGGACGAACAGATCGGAAGAGCACA754220.2215720167662877No Hit
GATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTG733610.21551728569902193Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG673130.19774969060206735No Hit
AGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACAC626930.18417722212522705No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA591580.17379222730582652No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC567140.16661233272630321No Hit
CAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCAGATCGGAAGAGC562880.16536084537324391No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAGATCGGAAGAGCACACG557080.16365694240428993No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT497610.14618606144503252No Hit
ATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGAGATCGGAAGAGCACA487230.14313666268335282No Hit
AATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAAGATCGGAAGAGCACA479540.14087752236761902No Hit
ACTCCGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGAAGAGCACACG473540.1391148641238735No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG469550.13794269639178275No Hit
TATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGAACTC444230.1305042786031768Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCAAGATCGGAAGAGCACAC435320.12788673111121474No Hit
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCA430790.1265559241371869No Hit
TCGCTGCGATCTATTGAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT428040.12574803910880353Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGAGATCGGAAGAGCA418990.1230893629244874No Hit
AGAACGAAAGTCGGAGGTTCGAAGACGATCAGAGATCGGAAGAGCACACG414820.12186431544508429No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA411950.12102117725182601No Hit
TTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCAGATCGGAAGAGC408410.11998120888801617No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA406140.11931433651913247No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCAGATCGGAAGAGCA398950.11720208439037745No Hit
CTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAAC365460.10736351362653801Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTC363750.10686115602707057No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGC363440.10677008535114371No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG359150.10550978470686569No Hit
GCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTC358580.10534233217370985No Hit
ATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCAGATCGGAAGAGCA345790.10158493235079237No Hit
GATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGA344240.10112957897115812Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC342930.10074473192127369No Hit
TTCTGGGCTGTAGTGCGCTATGCCGATCGGGAGATCGGAAGAGCACACGT341200.10023649879432707No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)