FastQCFastQC Report
Fri 30 Jun 2023
SRR2052919.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052919.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11895393
Total Bases606.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC4399693.6986503934758606No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA2440382.051533732429017No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1495871.2575204535066644No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC1468211.2342677539111149No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1167960.9818591113383139No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG963240.809758870513988No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG934160.7853124314598097No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACAC870730.731989266769076No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG857050.720489016209889No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG850770.7152096614210225No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACG848460.7132677331467737No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG846130.7113089916407134No Hit
ATATTAAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACG770940.6480996466447136No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC618970.5203443047236859No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA592210.49784820055966206No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT544660.45787474192739996No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC536670.451157855818635No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACAGATCGGAAGAGCAC491870.41349621656047847No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACG488880.41098263840463284No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC469270.39449726461328344No Hit
TATATTAAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACAC459660.38641850672777267No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCA453680.38139135041608124No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC433000.36400646872280723No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT431720.36293042188685987Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACAC422130.3548684772331608No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTA396600.33340638682555507TruSeq Adapter, Index 25 (97% over 44bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC344850.2899021495128408No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC302450.25425809807208555No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT296290.24907962267408904No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC284640.23928591514378716No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT277910.23362826263915787No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC267890.2252048335015077No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAGATCGGAAGAGCACAC266990.22444823806998224No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC264360.2222372980867467No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC260120.21867289294267117No Hit
ATATATTAAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACA256060.2152598068849007No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC252750.21247721702006817No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG247990.20847566784888907No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA244130.2052307141092354No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG243380.2046002179162975No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT239090.2009937796926928No Hit
ATATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACA232910.1957984910628846No Hit
TTAAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCT213920.17983432745769726Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATATTAAATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACAC206050.17321832073980237No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGAGATCGGAAGAGCACA204330.17177238280399817No Hit
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCA198950.16724962344665703No Hit
ACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACACGTC196280.16500505699979817No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT194050.16313038165279617No Hit
ATTCCGATAACGAACGAGACTCTGGCATGCTAGATCGGAAGAGCACACGT191660.16112120045130077No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT188240.158246137811504No Hit
AGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACGT180490.15173101048447915No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACACG176420.14830951781080287No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC170120.14301334979012464No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACAC169840.14277796454476116No Hit
ACTCCGGATGTGCTGACCCCTGCGATTTCCCCAAGATCGGAAGAGCACAC168250.14144131261573284No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC162090.13626283721773633No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGAAGATCGGAAGAGCACACG162070.13624602398592464No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTAGATCGGAAGAGCACA159620.1341864030889942No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT159590.13416118324127668No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT158230.13301788347808266No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGCAGATCGGAAGAGCACACG157080.13205112264891122No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTAGATCGGAAGAGCACACG156550.13160557200590178No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT156090.1312188676742332No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTC155620.1308237567266588No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT155560.13077331703122377No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCTAGATCGGAAGAGCACAC155460.13068925087216537No Hit
ATATTAAATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACAC154560.12993265544063992No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCG153190.12878094906154006TruSeq Adapter, Index 25 (97% over 40bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA150940.12688946048272637No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT150860.12682220755547965No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTAGATCGGAAGAGCACAC149570.1257377541036265No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGT149570.1257377541036265No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTAGATCGGAAGAGCACA148520.12485505943351347No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA147340.12386307875662451No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGA145040.12192955709828165Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG143660.12076944410327595No Hit
ATATTAAATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACG142870.12010532144671471No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGT141560.11900405476304987No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTCAGATCGGAAGAGCACAC141270.11876026290178056No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC140750.11832311887467693No Hit
ATATTAAATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGT137480.11557415547346775No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG137390.1154984959303152No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAGATCGGAAGAGCACAC134900.11340524856976143No Hit
AACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACACGT132050.11100936303659745No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG130010.10929441339180639No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCT125850.10579726117497758Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGGAAGCTAAGCAGGGTCGGGCCTGGTAGATCGGAAGAGCACACGTC122930.10334252933047272No Hit
TGATCTCGGAAGCTAAGCAGGGTCGGGCCTGGTAGATCGGAAGAGCACAC119310.10029933437255918No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACA119230.10023208144531248No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)