FastQCFastQC Report
Fri 30 Jun 2023
SRR2052921.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052921.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14469150
Total Bases737.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA3405262.353462366483173No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1856921.2833649523296116No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1439960.9951932214400985No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG1081690.7475836521150171TruSeq Adapter, Index 1 (100% over 49bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT994120.6870617831731649No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC720280.4978039484005626No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC684420.473020184323198No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG669580.4627638803938034No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG653610.45172660453447505No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC519980.3593714903778038No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC513530.3549137302467664No Hit
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC511810.3537249942118231TruSeq Adapter, Index 1 (100% over 36bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC463990.32067536793799223No Hit
CGGGGTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC459270.31741325509791524TruSeq Adapter, Index 1 (100% over 36bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA455160.3145727288748821No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC454540.3141442310018211No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA431050.2979096906176244No Hit
TTGACGCGATGTGATTTCTGCCCAGTGCTCTGAGATCGGAAGAGCACACG416530.28787454688077735No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG412620.2851722457780865No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG410890.2839765984871261No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC376630.2602986353724994No Hit
GGGTCTGTAGCTCAGCTGGTTAGAGCACCAGATCGGAAGAGCACACGTCT341460.23599174796031558Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC333870.23074610464332737No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCA327140.2260948293438108No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT325500.22496138335700439No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT317880.21969500627196487No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTC317750.21960515994374238TruSeq Adapter, Index 1 (100% over 50bp)
GTCCCGTTCGTCTAGAGGCCTAGGACAAGATCGGAAGAGCACACGTCTGA301460.20834672389186648Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG300470.20766250954617238No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGAGATCGGAAGAGCACA256560.17731518437503238No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC251830.1740461602789383No Hit
GTCCCGTTCGTCTAGAGGCCTAGGAGATCGGAAGAGCACACGTCTGAACT237370.16405248407819395Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA219100.15142561933492985No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC216290.14948355639412128No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG206640.14281419433760795No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC197100.13622085609728285No Hit
AGACGATCAGATACCGTCGTAGTTCCGACCATAAGATCGGAAGAGCACAC189020.1306365612354561No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC186100.12861847447845934No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG182270.12597146342390536No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG180480.12473434859684225No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACAC176190.1217694197655011No Hit
ATTCGGCTGATCTGGCTGGCTAGGCGGGTGAGATCGGAAGAGCACACGTC171920.11881831344619413No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC169580.11720107953818988No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGAGATCGGAAGAGCACA159830.11046260492150542No Hit
TGACGCGATGTGATTTCTGCCCAGTGCTCTGAGATCGGAAGAGCACACGT150470.10399366928948832No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT147930.10223821026114181No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)