FastQCFastQC Report
Fri 30 Jun 2023
SRR2052922.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052922.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14972823
Total Bases763.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG279713018.681380258084932TruSeq Adapter, Index 6 (100% over 49bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC4084012.727615226600889TruSeq Adapter, Index 6 (100% over 50bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC3834052.5606727602403367No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA2401171.6036855574930657No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1281440.8558439514044881No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1220430.8150967923684131No Hit
TGTAGTATCTGTTCTTATCAGTTTAATATCTGATAGATCGGAAGAGCACA1057830.7065000367666138No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA882330.589287671403048No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC612870.4093216088909887Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC483070.32263120989275035No Hit
AATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAGCA482560.322290592762634No Hit
AGTGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGT444640.2969647073233952No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG437370.292109243527423No Hit
TGGTAAGTGTACTGGAAAGTGCACTTGGACGAACAGATCGGAAGAGCACA421220.28132303440707207No Hit
TAACTGTTAGTCCAAAGAGGAGATCGGAAGAGCACACGTCTGAACTCCAG410940.2744572616666877Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA391850.26170749497272494No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA384740.25695889145286765No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC374640.25021333652311256No Hit
ATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGAGATCGGAAGAGCACA352770.23560687253165286No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC350270.23393718071735703No Hit
AGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATAGATCGGAAGAGCA327320.21860940986212155No Hit
ACTCCGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGAAGAGCACACG323230.2158777940539336No Hit
AATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAAGATCGGAAGAGCACA304100.20310131229094208No Hit
AAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGAAGATCGGAAGAGCA292230.19517361555666557No Hit
TGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAGCACACGTCTG274070.1830449742176208Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT253040.16899952667576448No Hit
TGTAGTATCTGTTCTTATCAGTTTAATATCTGATACAGATCGGAAGAGCA250390.16722965335261095No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG230020.15362500444972868No Hit
ATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAAGATCGGAAGAGCACAC228630.1526966558009802No Hit
TGGCTAAGATCAAGTGTAGTATCTGTTCTTAAGATCGGAAGAGCACACGT224820.1501520454759934No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC223900.14953759888833254No Hit
AGTGTAGTATCTGTTCTTATCAGTTTAATATCTGAAGATCGGAAGAGCAC221040.14762747145277813No Hit
TTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCAGATCGGAAGAGC213640.14268518368246255No Hit
TTCTTGACGACCATAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT209890.14018064596101884Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC208900.1395194480025577No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACAGATCGGAAGAGCACACGT207070.13829723359449317No Hit
CAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCAGATCGGAAGAGC206320.13779632605020442No Hit
ATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAGCAC178980.11953657636906546No Hit
ATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCAGATCGGAAGAGCA178310.11908909896283419No Hit
TGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGAGATCGGAAGAGC174980.11686506946619217No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCAGATCGGAAGAGCACA173120.1156228187563561No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG168320.11241701047290814No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAGATCGGAAGAGCACACG162450.1084965740929416No Hit
AGGGCTCCATAGCTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACG160410.10713410557247623No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG158370.10577163705201083No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)