FastQCFastQC Report
Fri 30 Jun 2023
SRR2052923.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052923.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3399547
Total Bases173.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC876742.5789906714041604No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG591811.7408495896659173No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG498471.4662835960202933No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA483081.4210128584779091No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACG417431.227898893587881No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG415551.222368745012203No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT328290.9656874871857927No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG317340.9334773133008604No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACAC315990.9295061959725811No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC268430.789605203281496No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC241320.7098592841928645No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG233080.685620760648404No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT232560.6840911450849186No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA207820.6113167430837109No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC205530.6045805514675926No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC203050.597285461857124No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC173910.5115681589341168No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC161260.4743573187839438No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACG157950.46462072740868127No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT150820.44364734477858375No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA149380.43941148629508575No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTAGATCGGAAGAGCACA148570.43702881589811826No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTA145650.4284394361954696TruSeq Adapter, Index 20 (97% over 44bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGAGATCGGAAGAGCACA126210.3712553466682473No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC125420.36893150763910604No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACACG118090.34736981133074496No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT110660.3255139581832521No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC106380.31292404546841096No Hit
ATATTAAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACG99390.292362482413098No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCTAGATCGGAAGAGCACAC93640.2754484641630194No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAGATCGGAAGAGCACAC91740.2698594842195151No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC89290.262652641660786No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCACA88900.26150542998817194No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC85440.2513276033542116No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTAGATCGGAAGAGCACAC84760.24932733684811534No Hit
TATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACAC83240.24485615289331197No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG82990.24412076079548245No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC80080.23556079677674704No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC74090.21794080211275207No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA74000.21767606095753345No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGCAGATCGGAAGAGCACACG69800.20532147371399778No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG68570.2017033445926766No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG66080.19437883929829475No Hit
TATATTAAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACAC63510.18681900853260744No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC62580.1840833499286817No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTCAGATCGGAAGAGCACAC61790.18175951089954046No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC61050.1795827502899651No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC58900.17325837824863136No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTAGATCGGAAGAGCACACG58150.17105220195514284No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACAC56270.16552205337946496No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACG52960.15578546200420232No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC52450.15428526212463012No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG52080.15319688181984248No Hit
TCCCTGTGGTCTAGTGGCTAGGATTCGGCGCTAGATCGGAAGAGCACACG50890.14969641543417403No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT50650.14899043902025771No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAAGATCGGAAGAGCACAC49480.1455488040024156No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCA48330.1421660003523999No Hit
ATATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACA48310.14210716898457354No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGAAGATCGGAAGAGCACACG47970.14110703573152542No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGT47310.1391656005932555No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG46480.13672409882846157No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACA45670.13434142843149396No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG44240.13013498563190917No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGT44040.12954667195364558No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC43940.1292525151145138No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAGATCGGAAGAGCACACG42450.12486957821144995No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTAGATCGGAAGAGCACA40760.11989832763012248No Hit
ATATTAAATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACAC40410.11886877869316118No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCCAGATCGGAAGAGCAC39710.11680968081923856No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT39640.11660377103184631No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCAGATCGGAAGAGCACA39570.11639786124445403No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG38790.11410343789922599No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT38480.11319155169791739No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTAGATCGGAAGAGCACA37830.11127953224356069No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCG37540.11042647741007845TruSeq Adapter, Index 20 (97% over 40bp)
TCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCAC37130.10922043436963806No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGTAGATCGGAAGAGCACACG36540.10748490901876044No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAGATCGGAAGAGCACA35720.10507282293787967No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC35290.10380794852961292No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACAGATCGGAAGAGCAC34900.10266073685699889No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC34040.10013098804046539No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)