FastQCFastQC Report
Fri 30 Jun 2023
SRR2052924.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052924.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6698199
Total Bases341.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC76041611.352544168962433No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA3408955.089353123130561No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC2443313.647711870011625No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG590640.8817892690258979No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA561900.8388822129650074No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA524500.7830463084181285No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA419920.6269147870942622No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC348930.5209310741588896No Hit
AGTGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGT277870.4148428555198196No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC243040.36284380323725823No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC236920.3537070188568599No Hit
ATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGAGATCGGAAGAGCACA170220.25412801261951157No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC168200.25111227659853047No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACAGATCGGAAGAGCACACGT153300.22886749109723376No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGT141630.21144489735225844No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA113920.17007556807434354No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT111340.16622378642378347Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA108720.16231228722825344No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCACA108000.16123737141879482No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAGATCGGAAGAGCACACG100730.15038370762051112No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCAC99860.14908485101741528No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT98750.14742768914449988No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACA97530.14560630402291722No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT83520.12469023389720132No Hit
TGGTAAGTGTACTGGAAAGTGCACTTGGACGAACAGATCGGAAGAGCACA76910.11482190959092138No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA72800.10868593184526168No Hit
TCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGAGATCGGAAGAGCACAC67850.1012958856552336No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)