FastQCFastQC Report
Fri 30 Jun 2023
SRR2052925.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052925.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751226
Total Bases38.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG127731.700287263752852No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC124441.656492187437602No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG107231.4274000101167958No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC94291.2551482509923777No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA72540.9656215306711963No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG69620.9267517364947433No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACG66500.8852196276486703No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC63520.8455511390713315No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG62610.8334376073245602No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT61110.8134702473024096No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACAC55060.7329352285464028No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACG54790.7293411037424157No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA45960.61179991107869No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA45440.6048778929376779No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC44700.5950273286600837No Hit
ATATTAAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACG42500.5657418672942629No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG40840.5436446555364165No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC39950.5317973552566072No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT36920.4914632880118633No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT36810.48999901494357223No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC34980.4656388357165486No Hit
TATATTAAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACAC27490.3659351513392774No Hit
TATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACAC27150.3614092164009233No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC25830.3438379395814309No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTA25120.3343867225042797TruSeq Adapter, Index 21 (97% over 40bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT23910.31827971875307826No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC22370.2977798957970038No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA21750.28952672032118165No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC21250.28287093364713145No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGAGATCGGAAGAGCACA21140.28140666057884045No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC20860.27767942004137236No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG20640.2747508739047903No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACAC20040.2667639298959301No Hit
ATATTAAATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACAC19880.26463407816023404No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGT19880.26463407816023404No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC19790.263436036558905No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG19440.25877698588706993No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTAGATCGGAAGAGCACA19360.2577120600192219No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG19180.25531597681656387No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG19020.2531861250808678No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTAGATCGGAAGAGCACACG18050.24027389893321052No Hit
ATATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACA17790.23681288986270443No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC16740.2228357378471991No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAGATCGGAAGAGCACAC16730.2227026221137181No Hit
ATATATTAAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACA16230.21604683543966796No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCTAGATCGGAAGAGCACAC14870.19794309568625154No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG14620.19461520234922647No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGAAGATCGGAAGAGCACACG14230.18942368874346735No Hit
TATATTAAATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACA13510.17983935593283512No Hit
AACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACACGT12610.16785893991954484No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT12290.16359923644815277No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC12080.1608038060450517No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTAGATCGGAAGAGCACA11950.15907330150979865No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACACG11700.15574540817277358No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA11280.15015454736657144No Hit
ATATTAAATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACG10920.1453623809612553No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT10470.1393721729546102No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC10360.13790789988631916No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC10150.1351124694832181No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTAGATCGGAAGAGCACAC10120.1347131222827751No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGCAGATCGGAAGAGCACACG9770.13005407161094No Hit
TATATTAAATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACA9600.12779110414176292No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC9590.12765798840828194No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACG9550.1271255254743579No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGT9200.1224664748025228No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT9000.11980416013290275No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCA8890.11833988706461171No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTAGATCGGAAGAGCACA8650.11514510946106765No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCACA8600.11447953079366262No Hit
TTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTAGATCGGAAGAGCAC8470.11274902625840959No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC8290.11035294305575152No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAGATCGGAAGAGCACA8250.10982048012182753No Hit
ATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCAGATCGGAAGAGCAC8190.10902178572094151No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACA8030.10689193398524545No Hit
ATTCCGATAACGAACGAGACTCTGGCATGCTAGATCGGAAGAGCACACGT7920.10542766091695442No Hit
AGACCCGCGGGTGCTTTCCAGCTCTTTTTTTAGATCGGAAGAGCACACGT7890.1050283137165114No Hit
TCCCTGTGGTCTAGTGGCTAGGATTCGGCGCTAGATCGGAAGAGCACACG7850.1044958507825874No Hit
TATATTAAATGGATTTTTGGAAATAGGAGATGAAGATCGGAAGAGCACAC7640.10170042037948633No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC7590.10103484171208132No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)