FastQCFastQC Report
Fri 30 Jun 2023
SRR2052927.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052927.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6813487
Total Bases347.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1759312.582099298053992No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC1505852.2101018171752584No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1177861.7287183493562106No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1076621.5801307025316111No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1009111.4810478100273765No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACAC973621.4289599437116411No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC942121.3827281097035924No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG913171.34023885273429No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACG844291.2391452423700229No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG830281.2185830838159668No Hit
ATATTAAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACG691431.0147960948630268No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT552800.8113319949095081No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA539780.7922228368528479No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACG537670.7891260378129437No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA505760.7422924561241548No Hit
AACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACACGT436530.6406851587153538No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC411440.6038611360086252No Hit
TATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACAC407490.598063810791743No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC395960.5811414918675268No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG393460.5774722986922849No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT378920.5561322711850775No Hit
TATATTAAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACAC377950.5547086242330835No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG307180.4508411038283334No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC305900.44896247692260954No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT294880.43278867340614285No Hit
ACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACACGTC282160.4141198185305116No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC280080.41106704980871034No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA278970.40943792803890283No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC257530.37797092736802756No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTA245730.3606523355808854TruSeq Adapter, Index 22 (97% over 40bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT242330.35566223286255627Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC240220.35256543382265204No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACAC239870.3520517467781182No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT226010.3317097398145766No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTAGATCGGAAGAGCACACG223870.32856891045656944No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGAAGATCGGAAGAGCACACG199700.29309515083832993No Hit
ATATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACA185210.27182850719462737No Hit
ATATTAAATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACAC182370.26766030374755245No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG164960.24210804247516726No Hit
ATATTAAATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACG159420.233977110398831No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAGATCGGAAGAGCACAC159340.2338596962172233No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC156810.2301464727238784No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGAGATCGGAAGAGCACA154040.22608100668571027No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC150810.22134040910329764No Hit
ATATATTAAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACA147840.21698140761111012No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC147480.2164530437938753No Hit
ATTCCGATAACGAACGAGACTCTGGCATGCTAGATCGGAAGAGCACACGT146020.214310234979534No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT143690.21089054694020842No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTC142650.2093641625793078No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACAGATCGGAAGAGCAC141440.20758827308249064No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG133530.19597894587602502No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACACG132340.19423240992460983No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG126990.18638033652959196No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGCAGATCGGAAGAGCACACG126240.18527957857701938No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACAC123090.18065639517621448No Hit
ATATTAAATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGT122380.17961434431444573No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCG121510.17833746508946155TruSeq Adapter, Index 22 (97% over 36bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTAGATCGGAAGAGCACA116840.1714834122381095No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTAGATCGGAAGAGCACAC116620.1711605232386882No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC116030.1702945936493311No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC115920.17013314914962047No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTAGATCGGAAGAGCACA113460.16652266306518235No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTCAGATCGGAAGAGCACAC109880.1612683784382358No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGT109020.16000617598595257No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT108720.15956587280492351No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT108330.1589934786695858No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT108220.15883203416987512No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGT107470.15773127621730254No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA106730.1566451950374309No Hit
TTAAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCT106230.15591135640238252Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACCCGCGGGTGCTTTCCAGCTCTTTTTTTAGATCGGAAGAGCACACGT102290.15012870795820113No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAAGATCGGAAGAGCACACGT100850.14801525268926177No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG98200.14412590792350524No Hit
TATATTAAATGGATTTTTGGAAATAGGAGATGAAGATCGGAAGAGCACAC98020.14386172601488784No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC96930.1422619577904823No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC95710.14047139152096424No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT94480.13866614847874517No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAGATCGGAAGAGCACACGTC93400.13708105702704063No Hit
TATATTAAATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACA92360.13555467266613996No Hit
AGACCCGCGGGTGCTTTCCAGCTCTTTTTTAGATCGGAAGAGCACACGTC91560.13438053085006252No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT90090.13222304526302023No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC89750.13172403499118732No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCTAGATCGGAAGAGCACAC89320.13109293376504572No Hit
TATATTAAATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACA88460.12983073131276246No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGATCGGAAGAGCACACG87640.1286272359512831No Hit
TATATTAAATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACAC87440.12833370049726373No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCT86850.12746777090790662Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC85760.12586800268350112No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTAGATCGGAAGAGCACACG85450.12541302272977112No Hit
ACTCCGGATGTGCTGACCCCTGCGATTTCCCCAAGATCGGAAGAGCACAC85270.12514884082115368No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGATCGGAAGAGCACACGT83140.12202268823584751No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACA82240.12070177869276039No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACAGATCGGAAGAGCACACGT80960.1188231517870365No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGT77890.11431738256783934No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC77120.1131872710698648No Hit
TATATTAAATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACG74160.1088429463503783No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC73850.1083879663966483No Hit
AGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACGT73670.10812378448803088No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC72640.10661207689983117No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGA72500.10640660208201763Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATATTAAATGGATTTTTGGAGCAGGGAGATAGATCGGAAGAGCACACGTC69870.10254661086166306No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTAGATCGGAAGAGCACA68170.1000515595024985No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)