FastQCFastQC Report
Fri 30 Jun 2023
SRR2052928.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052928.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6242440
Total Bases318.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC2586464.143347793491007No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1376732.2054356950166922No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA983931.5761945649457585No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC875891.4031212154221746No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC662391.0611075156509313No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA409270.6556250440532868No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA408670.654663881430979No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG351340.5628247928694549No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA294970.4725235645036236No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC277600.4446979065878086No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA274890.4403566554103844No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCACA257850.4130596369368388No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC253440.40599509166287545No Hit
AGTGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGT251080.4022145186817975No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT214620.34380786999955143No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACAGATCGGAAGAGCACACGT188910.3026220516336561No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT166700.26704301523122365Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA135700.21738294641197994TruSeq Adapter, Index 14 (97% over 44bp)
TGGTAAGTGTACTGGAAAGTGCACTTGGACGAACAGATCGGAAGAGCACA129600.20761112641851584No Hit
TGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGTCT120590.19317766770685824Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGT119270.19106310993778072No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACA117300.18790729266120298No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA112910.18087478614131652No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA110720.17736654256989254No Hit
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA104150.16684181185562055Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT95090.1523282562587706No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAG91660.14683360993457686No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAGATCGGAAGAGCACACG90340.14471905216549938No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAGATCGGAAGAGCAC86470.13851955325161316No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC86190.13807101069453612No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCAGATCGGAAGAGCACACGTCT85350.136725383023305Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCA80000.12815501630772583No Hit
TGCGGGCCGCCGGTGAAATACCACTACTCTGAGATCGGAAGAGCACACGT79280.12700162116095629No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCAGATCGGAA77470.12410211391699401No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA72820.11665310359410742No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA69800.11181525172849079Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTGCTTAGTTGAACAGGGCCCTGAAGCGCAAGATCGGAAGAGCACACGT68340.10947642268087478No Hit
ATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGAGATCGGAAGAGCACA63720.10207547048910362No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC62990.10090605596529562No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)