FastQCFastQC Report
Fri 30 Jun 2023
SRR2052929.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052929.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3488237
Total Bases177.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1640054.701658746237713No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1550394.444623458784481No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA833722.390089893547944No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG662681.8997562378932396No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA578211.6575995266376684No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACG348740.9997600507075637No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC294310.8437213411818061No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC252990.7252660871380012No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC251110.7198765450856693No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC235350.6746961287320787No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC229230.6571514492851259No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG228190.654170000490219No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAGATCGGAAGAGCACAC190900.5472678605266786No Hit
ATATTAAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACG186850.5356574108926658No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC181350.5198901336119077No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG177020.5074769862254199No Hit
TATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACAC176540.5061009329354628No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACAGATCGGAAGAGCAC176070.5047535474223799No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACG173950.49867597872506947No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA164060.47032354739657883No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG160900.4612645299043614No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG139860.40094752736124295No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACAC137830.39512796865579947No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA136340.3908564699015577No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT130360.3737131393308425No Hit
TATATTAAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACAC130000.3726810993633747No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC129880.3723370860408854No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG128870.3694416405766007No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC120340.3449880269029885No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG117180.33592900941077114No Hit
ATATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACA108210.31021401355469824No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA106390.30499647816361103No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT98860.2834096421774094No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT97180.2785934556625596No Hit
AACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACACGT96470.2765580435044981No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTAGATCGGAAGAGCACAC96170.27569801019827495No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGAAGATCGGAAGAGCACACG95940.2750386513301705No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGATAGATCGGAAGAGCACACGTC91170.2613641217612221No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTAGATCGGAAGAGCACACG88240.2529644631371091No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT88140.25267778536836805No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA87570.251043722086544No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT84640.24264406346243103No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG83400.23908925913004192No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTC76150.21830512089631524No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT74790.2144063032414369No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG74720.21420562880331814No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC74540.2136896088195842No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACAC72590.2080993923291336No Hit
ATATATTAAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACA72330.20735403013040685No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTAGATCGGAAGAGCACACG67210.1926761283708647No Hit
ACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACACGTC63320.1815243631668376No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC61280.17567613668452No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACAC58000.1662731058698133No Hit
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCA56720.1626036304299278No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT56640.16237428821493494No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTAGATCGGAAGAGCACA55250.15838946722943423No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC54360.15583803508763883No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT52720.1511365196802855No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT50330.14428492100737422No Hit
ATATTAAATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACG47950.13746199011133706No Hit
TATATTAAATGGATTTTTGGAAATAGGAGATGAAGATCGGAAGAGCACAC47680.1366879601357362No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG47250.13545524573014964No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT46040.13198644472838283Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT45940.13169976695964178Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC45520.13049572033092935No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGT44920.12877565371848299No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTA44830.12851764372661606TruSeq Adapter, Index 7 (97% over 36bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTAGATCGGAAGAGCACA44320.12705558710603668No Hit
TAGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACA43760.12545019160108672No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAGATCGGAAGAGCA43370.12433214830299662No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTAGATCGGAAGAGCACA42870.12289875945929132No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA42670.12232540392180921No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTAGATCGGAAGAGCACA40860.11713653630759607No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC40220.11530179858765331No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACTAGATCGGAAGAGCAC39700.11381107419019981No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCA38390.11005559541969197No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTCAGATCGGAAGAGCACAC38290.1097689176509509No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAAGATCGGAAGAGCACACGT38020.10899488767535004No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGAGATCGGAAGAGCACA37930.10873687768348309No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC37050.10621411331856179No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC35580.10199995011806824No Hit
ATATTAAATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGT34940.10016521239812548No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)