FastQCFastQC Report
Fri 30 Jun 2023
SRR2052930.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052930.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5860692
Total Bases298.8 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1475732.518013231202049No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1435932.4501031618791775No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1419522.422103055407109No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1069631.825091644467923No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC973161.6604865091016556No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA745701.272375344071997No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT463460.7907939881502047No Hit
AGTGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGT364410.6217866422599926No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA340900.5816719254313313No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA324440.553586504801822No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACAGATCGGAAGAGCACACGT316870.5406699413652859No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC288940.4930134530188585No Hit
CTGATGTTCGCCGACCGTTGACTATTCTCTACAGATCGGAAGAGCACACG274190.4678457765738244No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC245670.41918258116959567No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA236650.4037919071672765No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC234270.3997309532730947No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC226480.38643900754381905No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG167450.28571711326921806No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG166270.28370369915361526No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACAGATCGGAAGAGCAC165290.2820315416677758No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT138440.23621783912206956Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA110210.18804946583099744No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA109500.18683800479533816TruSeq Adapter, Index 15 (97% over 40bp)
AGTGCTTAGTTGAACAGGGCCCTGAAGCGCAAGATCGGAAGAGCACACGT107450.18334012434026561No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC107220.18294767921603797No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTAGATCGGAAGAGCA105230.17955217574989438No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC101770.17364843605499145No Hit
TGCGGGCCGCCGGTGAAATACCACTACTCTGAGATCGGAAGAGCACACGT101210.17269291749165458No Hit
ACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACACGTC98810.16859783793449648No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAGATCGGAAGAGCAC94450.16115844340565927No Hit
TGGTAAGTGTACTGGAAAGTGCACTTGGACGAACAGATCGGAAGAGCACA93980.1603564903257158No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTAGATCGGAAGAGCACAC93890.16020292484232238No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT93810.16006642219041708No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACAC93510.15955453724577234No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC90160.15383848869723915No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG89790.1532071639321773No Hit
AGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACAC88580.1511425613221101No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC87540.14936802684734157No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG86080.1468768534500704No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTAGATCGGAAGAGCACACG85600.1460578375386388No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC85210.1453923871106006No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT85010.1450511304808374No Hit
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA82530.14081954827177404Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGATGTTCGCCGACCGTTGACTATTCTCTAGATCGGAAGAGCACACGTC81370.13884025981914763No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACA80820.1379018040872989No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA78740.1343527351377619No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCACA75130.1281930529705366No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTCAGATCGGAAGAGCACACGT73330.125121743302668No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC72570.12382496810956795No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGC72110.12304007786111265No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA71750.12242581592753893No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA71580.12213574779224023No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTAGATCGGAAGAGCAC69660.11885968414651375No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCAGATCGGA68550.11696570985132815No Hit
TGTAGTATCTGTTCTTATCAGTTTAATATCTGATAGATCGGAAGAGCACA65990.11259762499035951No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC65990.11259762499035951No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC65770.11222224269762No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG65380.11155679226958182No Hit
TCGATGTTGGATCAGGACATCCCGATGGTGCAGATCGGAAGAGCACACGT64270.1096628179743962No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG61790.10543123576533284No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAG61270.10454396852794857No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC61020.1041173977407446No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)