FastQCFastQC Report
Fri 30 Jun 2023
SRR2052931.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052931.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4934701
Total Bases251.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1006432.039495402051715No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1006182.0389887857440603No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC990552.0073151341894877No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC614061.2443712395138025No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA378600.7672197363122912No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA336150.6811962872725217No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC311580.6314060365562169No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA274270.5557986188018281No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA252670.5120269698204613No Hit
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCA247850.5022594074088784No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACAC243140.4927147561726637No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC232740.47163951777422786No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTA216100.4379191363367304TruSeq Adapter, Index 27 (97% over 44bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC211260.42811104462053523No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC209360.42426076068235946No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG195570.3963158051521257No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGAGATCGGAAGAGCACA166570.3375483134641795No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG162720.329746422326297No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCA142260.2882849437078356No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC135470.27452524479193374No Hit
TTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCAGATCGGAAGAG132960.2694388170630804No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG128830.26106951566062464No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAG122800.24884993031999303No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCG105030.21283964317189633TruSeq Adapter, Index 27 (97% over 40bp)
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCA104190.21113741237817651No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAGATCGGAAGAGCA101270.20522013390476948No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTCAGATCGGAAGAGC99550.2017346137081051No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG91770.18596871421389058No Hit
TATATTAAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACAC91210.18483389368474404No Hit
TTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCAAGATCGGAAGAG89810.18199684236187766No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC87690.17770073607296571No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC82510.16720364617836014No Hit
TAGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACA80590.16331283293557197No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACAGATCGGAAGAGCAC76980.15599729345303798No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTAGATCGGAAGAGCACA75000.15198489229641268No Hit
AGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATAGATCGGAAGAGCA74830.15164039320720749No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAGATCGGAAGAGC74760.15149854064106416No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACA73990.14993816241348767No Hit
TTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCAGATCGGAAGAGC73680.14930995819199583No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG73510.14896545910279063No Hit
ATATATTAAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACA73240.14841831349052353No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCCAAGATCGGAAGAG72490.14689846456755942No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG71100.14408167789699924No Hit
ATATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACA69600.141041980051071No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA69190.14021112930651725No Hit
TATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACAC68270.1383467812943479No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTAGATCGGAAGAGCACA67700.1371916961128952No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAACCAAGATCGGAAGA67330.1364419039775662No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACAGATCGGAAGAGCAC65300.13232817955940998No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC65240.13220659164557286No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAACCAGATCGGAAGAG64320.13034224363340352No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGCGAGATCGGAAGAGCACA59960.1215068552279054No Hit
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGC59610.12079759239718882No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAGATCGGAAGAGCACA58670.11889271508040711No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG57680.11688651450209445No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAGATCGGAAGAGCACAC55630.11273226077932585No Hit
TTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGTAGATCGGAAGAG55450.11236749703781444No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA53760.10894277079806862No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCACA51790.10495063429374953No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)