FastQCFastQC Report
Fri 30 Jun 2023
SRR2052933.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2052933.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4141321
Total Bases211.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC1165262.813739867061742No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1060342.5603907545442626No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA708681.7112414130660243No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG696091.680840485439308No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACG687301.659615373935032No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT633571.5298741633406345No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG578541.3969938577569814No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACGT409950.9899015314195638No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC409740.9893944468443765No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC380950.9198755662746259No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC367590.8876153285388889No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG315880.7627517886201045No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACG313120.756087248489069No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC280090.6763300888774378No Hit
ATATTAAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACG271930.6566262310987243No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACAC269660.6511448883097929No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA269190.6500099847367543TruSeq Adapter, Index 19 (97% over 40bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA254980.6156972618157347No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC240240.5801047540144799No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTAGATCGGAAGAGCACA218930.5286477430752168No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA187210.4520538253373742No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA184970.4466449232020411No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC169420.4090965177536346No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACACG169270.4087343144856436No Hit
TATATTAAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACAC161320.3895375412821175No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC148490.35855708842661554No Hit
TATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACAC147210.3554662872064252No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG146100.35278598302329134No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC134840.32559659103942923No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTAGATCGGAAGAGCACAC130810.3158653965727361No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGAGATCGGAAGAGCACA130320.31468219923063195No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC126080.3044439201887514No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC115510.2789206632376481No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCG115250.2782928442397969TruSeq Adapter, Index 19 (97% over 36bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC105430.2545806036286489No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACG102890.24844729495733367No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAGATCGGAAGAGCACAC99730.2408168794449887No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT98420.23765363757120012No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCTAGATCGGAAGAGCACAC96520.23306572950998003No Hit
ATATATTAAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACA93030.22463846680805472No Hit
ATATATTAAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACA92390.22309306619795957No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG88060.21263746519528431No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG85190.20570730933438872No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG81580.1969902840180706No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG74440.17974940846169615No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGCAGATCGGAAGAGCACACG72960.17617566955085107No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAGATCGGAAGAGCACACG71060.17158776148963095No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGAAGATCGGAAGAGCACACG70470.17016309530219947No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCACA70330.16982503891874112No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC69270.16726546915827098No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG67270.16243609225172354No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGTAGATCGGAAGAGCACACG65510.1581862405739618No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAAGATCGGAAGAGCACAC64590.15596472719694995No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCA63280.15280148532316137No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTAGATCGGAAGAGCACACG62800.15164243486558998No Hit
ATATTAAATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACAC62160.1500970342554948No Hit
TCCCTGTGGTCTAGTGGCTAGGATTCGGCGCAGATCGGAAGAGCACACGT59850.14451910392843248No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC58780.1419353872834296No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC58010.14007607717440884No Hit
ATATTAAATGGATTTTTGGAGCAGGGAGATGAAGATCGGAAGAGCACACG57300.1383616483725845No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC56800.13715430414594762No Hit
TCCCTGTGGTCTAGTGGCTAGGATTCGGCGCTAGATCGGAAGAGCACACG55460.13391862161856086No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACAGATCGGAAGAGCAC54450.13147978628075438No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC54410.1313831987426234No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAGATCGGAAGAGCACA53710.12969291682533182No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACA53670.12959632928720088No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTCAGATCGGAAGAGCACAC53170.128388985060564No Hit
TCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCAC51140.12348716750041834No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTAGATCGGAAGAGCACA50830.1227386140799035No Hit
ACTCCGGATGTGCTGACCCCTGCGATTTCCCCAAGATCGGAAGAGCACAC50710.12244885146551064No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT49580.11972025351331132No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA49500.11952707843704943No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC48270.11655701163952276No Hit
ATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCAGATCGGAAGAGCAC47350.11433549826251092No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA46520.11233130684629372No Hit
ATATTAAATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACAC45110.10892659612717777No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC43330.10462845068035054No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAAGATCGGAAGAGCACACGT42520.10267255303319882No Hit
TATATTAAATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACA42290.10211717468894586No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT42120.10170667765188933No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGT41580.10040274588712153No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)